- Open Access
Adiponectin and adiponectin receptor 1 overexpression enhance inflammatory bowel disease
© The Author(s). 2018
- Received: 20 November 2017
- Accepted: 6 February 2018
- Published: 14 March 2018
Adiponectin (ADN) is an adipokine derived from adipocytes. It binds to adiponectin receptor 1 and 2 (AdipoR1 and R2) to exert its function in regulating whole-body energy homeostasis and inflammatory responses. However, the role of ADN-AdipoR1 signaling in intestinal inflammation is controversial, and its role in the regulation of neutrophils is still unclear. Our goal was to clarify the role of AdipoR1 signaling in colitis and the effects on neutrophils.
We generated porcine AdipoR1 transgenic mice (pAdipoR1 mice) and induced murine colitis using dextran sulfate sodium (DSS) to study the potential role of AdipoR1 in inflammatory bowel disease. We also treated a THP-1 macrophage and a HT-29 colon epithelial cell line with ADN recombinant protein to study the effects of ADN on inflammation.
After inducing murine colitis, pAdipoR1 mice developed more severe symptoms than wild-type (WT) mice. Treatment with ADN increased the expression of pro-inflammatory factors in THP-1 and HT-29 cells. Moreover, we also observed that the expression of cyclooxygenase2 (cox2), neutrophil chemokines (CXCL1, CXCL2 and CXCL5), and the infiltration of neutrophils were increased in the colon of pAdipoR1 mice.
Our study showed that ADN-AdipoR1 signaling exacerbated colonic inflammation through two possible mechanisms. First, ADN-AdipoR1 signaling increased pro-inflammatory factors. Second, AdipoR1 enhanced neutrophil chemokine expression and recruited neutrophils into the colonic tissue to increase inflammation.
- Adiponectin receptor 1
Inflammatory bowel disease (IBD) is a chronic, idiopathic gastrointestinal tract inflammation, including severe forms such as Crohn’s disease and ulcerative colitis [1, 2]. Due to the dramatically increasing incidence worldwide, IBD has become a noticeable health problem over the past decades [3, 4]. In the USA and Europe, there are over 1 million and 2.5 million people with IBD, respectively [1, 5, 6]. Although IBD is not a life-threatening disease, the IBD patients have poor life quality due to loss of body weight, diarrhea, rectal bleeding and abdominal pain . Moreover, robust evidences suggest that IBD patients may have a higher risk to develop colon cancer [8–10].
Adiponectin (ADN) is an adipokine secreted from adipocytes, and various forms of ADN have been found in plasma. The full-length ADN includes 4 regions (a signal peptide, a variable region, a collagen-like fibrous domain and a c1q-like globular domain) and exists in multiple forms (trimer, hexamer and oligomer) [11, 12]. Globular ADN is formed when the collagen-like domain of full-length ADN is cleaved by elastase . ADN can trigger the activation of AMP-activated protein kinase (AMPK), peroxisome proliferator activated receptor alpha (PPARα) and p38 MAP kinase (p38 MAPK) through its receptors, adiponectin receptor 1 (AdipoR1) and 2 (AdipoR2) .
It is well known that ADN improves several different diseases, therefore, enhancing ADN signaling might be a potential therapeutic strategy for the metabolism-related diseases . However, the role of ADN and AdipoR1 in chronic inflammatory and autoimmune diseases, especially in IBD, is still controversial . Previous studies support the protective role of ADN and AdipoR1 in chemically-induced murine colitis [17–21]. However, others implicate ADN in exacerbation of murine colitis [22, 23]. Most studies use a similar experimental model (induction of murine colitis by DSS or 2,4,6-trinitrobenzene sulfonic acid in ADN knock-out mice). Despite this, the results are discrepant. The controversial results let us speculate that ADN might not be the only factor related to inflammation in ADN Knock-out mice.
The innate immune system is the first line of defense against invading pathogens. Neutrophils are one of the most abundant and important immune cells in the innate immune system . Neutrophils, as the effector cells of acute and chronic inflammation, play a role in the maintenance of intestinal homeostasis and pathogenesis of IBD . At the early stages of inflammation in patients with IBD, neutrophils infiltrate into the inflamed intestine, clear pathogenic microbes, and resolve inflammation. However, excessive neutrophil infiltration and accumulation in the colonic epithelium causes damage by production of reactive oxygen species (ROS) [25, 26]. Previous studies show that neutrophils play a crucial and dual role in IBD, and either depletion or upregulation of neutrophils can ameliorate murine colitis [26–28]. Hence, the dual role of neutrophils might lead to the controversial results in previous studies [26, 29]. Furthermore, previous studies demonstrate that ADN inhibits neutrophil apoptosis and enhances the expression of neutrophil-attracting chemokines (CXCL1 and CXCL2 in colonic cells; IL-8 in fibroblasts and hepatocytes) [22, 30–32]. Regardless, the relationship between ADN-AdipoR1, neutrophils and IBD is still unclear.
Earlier studies regarding the effects of ADN on intestinal inflammation mostly use ADN knock-out mice or ADN recombinant protein treatment [17–20, 22, 23]. It should be noted that development of ADN as a pharmacological agent is difficult due to its multiple forms and high level (2–20 μg/mL) in blood . In a previous review, it is suggested that regulation of AdipoRs might be one potential target for therapy . However, studies about the role of AdipoRs in colitis are still rare; only the study by Sinderi shows that short-term (2 days) AdipoR1 knock-down exacerbates colitis in mice . The effects of long-term overexpression of AdipoR1 on colitis are still unknown.
To study the physiological role of AdipoR1 in intestinal inflammation, we employed DSS to induce murine colitis in porcine AdipoR1 transgenic mice (pAdipoR1 mice). We also used recombinant ADN protein to investigate the role of ADN-AdipoR1 signaling in colitis in the THP-1 macrophage cell line and the HT-29 human colon epithelial cell line.
pAdipoR1 mice were generated as previously reported . Both wild type (WT) and transgenic mice were maintained under the same breeding conditions, and with the same diet fed ad libitum to ensure similar intestinal microflora. We used eight-week old pAdipoR1 female mice constructed on a genetic background of FVB/N. Eight week old female FVB/N WT mice were used for the control group.
pAdipoR1 and WT mice (n = 3 for each group) were given different doses of dextran sodium sulfate (DSS) (0.5, 1, or 2%) (MW = 36,000–50,000, MP Biomedicals, Illkirch, France) in drinking water to induced colitis. We observed the mice for diarrhea and sluggish behavior and recorded body weight and food intake every day. The 2% DSS group mice were euthanized with CO2 on the ninth day and the 0.5 and 1% DSS group on the 15th day.
Establishment of acute colitis
WT and pAdipoR1 mice (n = 8 for each group) were given 2% DSS in drinking water for 7 consecutive days to induce acute colitis and were euthanized with CO2 at the end of experiment. The body weight and clinical phenotypes as diarrhea, sluggish behavior and food intake were recorded daily. All the animal experiments were approved by the Institutional Animal Care and Use Committee (IACUC) at National Taiwan University.
To evaluate the severity of colitis, the whole large intestine was harvested, fixed in 10% formalin for 15 h, then, embedded in paraffin. Paraffin sections (4 μm) were stained with hematoxylin and eosin. The sections were also reacted with various antibodies, AdipoR1 (5512–1; Epitomics, Burlingame, CA, United States), CD68 (ab125212; abcam, Cambridge, United Kingdom), cyclooxygenase/cox2 (SP21; Spring Bioscience, Inc., Pleasanton, CA, United states) and neutrophil elastase (bs-6982R; Bioss, Inc.). The endogenous peroxidase activity and non-specific binding were blocked by 0.3% H2O2/ methanol and 10% calf serum/PBS. The signal was detected by a horseradish peroxidase (HRP)/ diaminobenzidine (DAB) system according to the manufacturer’s instructions (Dako, Carpinteria, CA, United States).
Protein extraction and western blotting analysis
The colon of WT and pAdipoR1 mice was homogenized in liquid nitrogen and total protein was extracted using the RIPA buffer (Cell Signaling, Beverly, MA, United States). Proteins were mixed with SDS-PAGE sample buffer and boiled for 5 mins. Proteins were separated by 10% SDS-PAGE and transferred to PVDF membranes. Membranes were blocked with 5% nonfat milk for 1 h at room temperature and incubated with antibodies against ADN (EPR3218, 1:1000, Epitomics, Burlingame, CA, United State) or β-actin (sc-47,778, 1:5000, Santa Cruz Biotechnology, Dallas, TX, United State) overnight at 4 °C. After washing with Tris buffered saline with Tween20, membranes were incubated with HRP-conjugated secondary IgG antibody (1: 5000, Cell signaling, Beverly, MA, United State) and the signal was detected using the ECL Western blotting substrate (Thermo Scientific, Rockford, IL).
Colon tissue culture and enzyme-linked immunosorbent assay
After euthanasia of the mice, 0.2 g of the terminal colon was cut into pieces and soaked in 3 mL RPMI-1640 medium (SH300027; GE Healthcare Life Sciences, Marlborough, MA, United States) for 15 h, then centrifuged at 12,000 X g for 10 mins. The supernate was collected. The concentrations of IL-6 and TNF-α in the supernate were determined using mouse-specific enzyme-linked immunosorbent assay (ELISA) kits (eBioseience, San Diego, CA, United States). The serum ADN, IL-6 and TNF-α levels were quantified using mouse ADN, IL-6 and TNF-α ELISA kits (R&D System, Inc., Minneapolis, MN, United States).
The human colon epithelial cell line, HT-29 (Food Industry Research and Development Institute, Hsinchu, Taiwan), was cultured in DMEM medium (SH30022; GE Healthcare Life Sciences) with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Waltham, MA, United States) and 1% glutamine (GE Healthcare Life Sciences). The human monocyte cell line, THP-1 (Food Industry Research and Development Institute), was cultured in RPMI-1640 medium (SH300027; GE Healthcare Life Sciences) with 10% FBS, 1% glutamine and 0.05 mM 2-mercaptoethanol. 2 × 106 cells were plated on a 100 mm plate and cultured in humidified 95% air plus 5% CO2 at 37 °C. Cells were subcultured every three days.
Adiponectin recombinant protein treatment
Full-length human adiponectin recombinant protein (ADN; mimics serum adiponectin by forming high molecular weight and hexameric species) was purchased from ENZO Life Sciences (ALX-522-063; ENZO Life Science, Farmingdale, NY, United States). After 6 h of serum deprivation, 1 μg/mL lipopolysaccharide (LPS; Sigma-Aldrich, St. Louis, MO, United States) or 5 μg/mL ADN were added to 5 × 105 HT-29 cells for 18 h. 4 × 105 suspended THP-1 cells were differentiated into plated macrophages by treating with 100 nM phorbol myristate acetate (PMA; Sigma-Aldrich) for 72 h. We added 1 μg/mL LPS or 5 μg/mL ADN for 18 h after 6 h’ serum starvation. Cells were harvested for RNA extraction and the culture media (conditioned medium) was collected for ELISA. The prostaglandin E2/PGE2 (ENZO Life Sciences), IL-8, TNF-α, IL-1 and IL-6 (eBioscience) expressions in conditioned media were detected using ELISA kits, as indicated above.
RNA extraction, cDNA synthesis and reverse transcription-quantitative polymerase chain reaction (qPCR)
Total RNA was extracted with TRIzol reagent (Thermo Fisher Scientific, Inc.) and cDNA was synthesized using a high capacity cDNA reverse transcription kit (Applied Biosystems, Foster, CA, United States). qPCRs were performed with the DyNAmo Flash SYBR Green qPCR Kit (Thermo Fisher Scientific, Inc.) using a C1000™ Thermal Cycler (Bio-Rad, Hercules, CA, United States). qPCR was performed with an initial denaturation at 95 °C for 5 mins followed by 40 cycles at 95 °C for 30 s, then 60 °C for 60 s and 72 °C for 30 s; terminal extension was at 72 °C for 7 mins. The primers were listed in Additional file 1: Table S1.
The immunohistochemical signal (brown color) score was quantified by image J (https://imagej.nih.gov/ij/download.html).
Data were expressed as mean ± SEM. A paired t test or one-way ANOVA followed by Tukey’s multiple comparison test were used for comparisons among groups. Means indicated by different letters were different at P ≤ 0.05.
The expression of adiponectin and adiponectin receptor 1
To examine the macrophage infiltration in the colonic tissue of mice, we detected the expression of the macrophage marker, CD68 by immunohistochemistry. There was greater macrophage infiltration in pAdipoR1 than in WT colon tissue (Fig. 2d). After 7 days of DSS induced murine acute colitis, the expression of CD68 was greater in the colon of pAdipoR1-DSS mice than in the colon of WT-DSS mice (Fig. 2d).
IL-6 and TNF-α in mouse serum and colonic tissue culture medium
The effect of adiponectin on the cyclooxygenase/prostaglandin E2 pathway
Adiponectin enhanced the expression of IL-8
IL-8 is a chemokine inducing chemotaxis in target cells, primarily in neutrophils in humans . In HT-29 and THP-1 cells, 18 h treatment with LPS or ADN enhanced mRNA expression and protein secretion of IL-8. The LPS treatment was more effective than the ADN treatment in the THP-1 cells (Fig. 4c and d; Fig. 5c and d).
The effect of adiponectin on pro-inflammatory cytokines
TNF-α, IL-1 and IL-6 are important pro-inflammatory cytokines in acute inflammation. In our study, LPS or ADN did not affect the mRNA expression or protein secretion of TNF-α or IL-6 in HT-29 cells (data not indicated). In THP-1 cells, TNF-α, IL-1 and IL-6 were increased by the inflammatory inducer, LPS. Like LPS, ADN treatment enhanced the level of TNF-α and IL-1 but not IL-6 (Fig. 5e-j).
Adiponectin receptor 1 enhanced the expression of neutrophil chemokines, cox2 and elastase in mice colon tissue
Our study showed that ADN and AdipoR1 enhanced the neutrophil chemokines (IL-8, CXCL1, CXCL2 and CXCL5) in human cell lines or mice colon. To investigate whether the increasing chemokines affect neutrophil infiltration, we detected the expression of neutrophil elastase, a marker for neutrophils in mice colon. Neutrophil infiltration was rarely detected in WT mice, but could be detected in pAdipoR1 mice. In the colitis groups, there was considerably more elastase expressed in the WT-DSS than in control-WT mice. However, colitis did not further elevate the elastase levels in the pAdipoR1-DSS compared to pAdipoR1 mice (Fig. 7b).
In summary, AdipoR1 overexpression increased the expression of cox2 and neutrophil chemokines to promote neutrophil infiltration into the colon of pAdipoR1 mice. The excessive cox2 and neutrophil infiltration might worsen murine colitis.
Numerous studies demonstrate that ADN functions as an anti-inflammatory agent and participates in improvement of metabolic inflammatory diseases, such as type 2 diabetes, obesity, and cardiovascular diseases [36–38]. Low adiponectinemia has been observed in patients with metabolic disease [39–42]. Therefore, ADN is considered a potential therapeutic target for metabolic disease . However, due to the rather great plasma concentration (2 to 20 μg/mL) of ADN and its multiple forms, the utility of ADN as a pharmacological agent is problematical . There are only a few studies regarding the effects of AdipoR1 on metabolic disease, therefore, we examined the therapeutic potential of AdipoR1 in this study. Both ADN and AdipoR1 proteins are highly conserved between humans, pigs and mice. The pig ADN has 83 and 81% identity with the human and mouse, respectively. The pig AdipoR1 has about 97% identity with both human and mouse forms . Moreover, previous study determined the crystal structure of human AdipoR1 and revealed its zinc-binding site and putative ADN-binding surface . The binding of ADN to the C-terminal extracellular region/CTR (residues 365–375) of AdipoR1 and the zinc-binding site (residues 187–212, 333–347) of ADN may have a role in the ADN-stimulated phosphorylation of AMPK and the up-regulation of UCP2 . The sequence alignment of human, pig and mouse AdipoR1 showed the CTR and zinc-binding site of mouse and pig AdipoR1 are identical (Additional file 2: Figure S1). Furthermore, previous studies showed that ADN binds to AdipoR1 to induce the phosphorylation of AMPK . We isolated porcine adipocyte and used the ADN treated-porcine adipocyte to confirm whether the mouse ADN can activate porcine AdipoR1. The porcine adipocyte was treated with 10 μg/mL murine ADN recombination protein (ALX-522-059; ENZO Life Science, Farmingdale, NY, USA) and the phosphorylation levels of AMPK were detected at 0, 5 and 15 min. The result showed the mouse ADN increased the level of phosphorylated AMPK at 15 min in porcine adipocyte (Additional file 3: Figure S2). It implies that mouse ADN could activate mouse and pig AdipoR1.
Previously, we established the pAdipoR1 mice carrying the transgenic porcine AdipoR1 conjugated with flag-tag (Additional file 4: Figure S3A). To confirm whether the mouse ADN binds to porcine AdipoR1, we performed co-immunoprecipitation(IP) using anti-ADN antibody (ab22554; Abcam, Cambridge, MA, USA) and detected the porcine AdipoR1 using anti-flag antibody (F1804; Sigma-Aldrich, St. Louis, MO, USA) in the colon of WT and pAdipoR1 mice. Flag is detectable in the protein of pAdipoR1 mice performed ADN IP (Additional file 4: Figure S3B). It suggests that mouse ADN binds to porcine AdipoR1 in pAdipoR1 mice. Moreover, we reported that the overexpression of pAdipoR1 resists the weight gain, hepatosteatosis, insulin resistance and heart hypertrophy in mice fed a high-fat/sucrose diet [33, 46, 47]. These results suggest that up-regulation of AdipoR1 might be a potential strategy for therapy of metabolism-related diseases.
In earlier studies, the prevalence of IBD was not considered gender-related . However, an extensive clinical study recently showed IBD was more prevalent in females compared with males . To avoid the interference of the menstrual cycle, previous studies usually used male mice. In this study, we used a DSS-induced colitis in both male and female mice. Both genders of pAdipoR1 mice showed more severe colitis symptoms than WT mice (data not shown). However, the difference is more significant in female mice. Therefore, we are the first to use female mice to study AdipoR1 effects in colitis.
The role of ADN in clinical and animal studies of IBD is controversial. Some studies show that the level of ADN in serum and mesenteric adipose tissue is increased in IBD patients [49–51]. Others demonstrate that ADN is down-regulated in serum and adipose tissue in IBD patients . However, some studies indicate that there is no correlation between IBD and the level of ADN in serum [53, 54]. For example, knockout of ADN suppresses chemically-induced colonic inflammation in mice [22, 23]; in contrast, the study by Nishihara shows that ADN displays protective effects against murine colitis .
Aberrant immunoregulation is a portion of the pathogenesis in IBD. The disturbance of pro- and anti-inflammatory cytokines and the infiltration of activated immune cells (e.g., T cells, B cells, natural killer cells, macrophages and neutrophils) into intestinal mucosa are involved in the aggravation of mucosal inflammation in IBD . In clinical studies, the high level of IL-8 (an chemoattractant for neutrophils), CXCR1 (the IL-8 receptor), and the high amount of neutrophils in serum are positively correlated with inflammation in IBD patients [55–61]. ADN enhances the IL-8 signaling through AdipoR1, but not AdipoR2 in hepatocytes, . Discontinued proliferation of plasma neutrophils coupled with the short lifespan of neutrophils, cause neutrophils to be present in the blood only a few days. Delayed apoptosis in neutrophils in IBD patients is relevant to pathological intestinal inflammation [62, 63]. Due to the effects of neutrophil chemotaxis and the delayed apoptosis in IBD, depletion of neutrophils and blockade of neutrophil adhesion may be an effective strategy for IBD therapy [27, 64, 65].
Previous studies demonstrate that ADN inhibits neutrophil apoptosis and enhances the expression of neutrophil-attracting chemokines (CXCL1 and CXCL2 in colonic cells; IL-8 in fibroblasts and hepatocytes) [22, 30–32]. In this study, we showed a requirement of AdipoR1 signaling in murine colitis through regulating of neutrophil chemotaxis. We observed the accumulation of neutrophils and the increased of CXCL1, CXCL2, and CXCL5 in the colon of pAdipoR1 mice. Moreover, ADN enhanced the expression and secretion of IL-8 in a human colonic epithelial and macrophage cell lines. We speculated that the activation of neutrophils could be the critical reason for the severe colitis phenotype in pAdipoR1 mice.
It is well known that the cox2/PGE2 pathway plays an important role in inflammation. Our data showed that ADN and AdipoR1 enhanced the expression of colonic cox2, like to Arsenescu’s previous report . However, contrary to our results, the data by Arsenescu shows that although ADN induces the expression of cox2, it mediates a protective effect toward colitis and that cox2 knock-out mice are more susceptible to DSS-induced colitis [17, 66]. The serum levels of cox2 and PGE2 are increased in IBD patients, which may relate to the pro-inflammatory function of cox2/PGE2 pathway [67, 68]. Previous studies show that ADN is a biomarker for rheumatoid arthritis, and that ADN induces the expression of cox2 and PGE2 in rheumatoid arthritis synovial fibroblasts [69, 70]. Our results implied that the ADN-AdipoR1 signaling enhanced colonic inflammation like occurrences in rheumatoid arthritis.
Moreover, the long-term overexpression of AdipoR1 might result in an imbalance of the colonic immune system by increased neutrophil chemotaxis. Our findings strengthen the view of the multifaceted role of ADN-AdipoR1 signaling that must be considered carefully in disease therapy in the future. However, there are limitation to this study  The ADN-AdipoRs signaling is complicated, and ADN exists full-length and in globular forms in plasma and binds to AdipoR1 and AdipoR2. We only tested the effects of full-length recombinant ADN protein in cell models and AdipoR1 in a mouse model. We have not verified what kind of ADN induced colitis in mice and the role of AdipoR2 in IBD is still unclear.  We observed that ADN-AdipoR1 signaling increases neutrophil chemotaxis, but we do not know whether the inhibition of neutrophil infiltration can improve colitis.
This research was supported by Ministry of Science and Technology, Taiwan. We thank our colleagues who provided insight and expertise that greatly assisted the research.
We thank Professor Shin-Torng Ding for the idea and direction, Professor Tang-Long Shen for assistance with cell culture and immunohistochemistry, Dr. Bing-Hsien Liu provided the porcine AdipoR1 transgenic mice, Professor Harry John Mersmann for editing the manuscript and Mrs. Yu-Shan Chen for assistance of animal care and experiment.
Ministry of Science and Technology, Taiwan.
Availability of data and materials
Y-JP: Perform the experiment and write the manuscript. T-LS: assistance with cell culture and immunohistochemistry. B-HL: provided the porcine AdipoR1 transgenic mice. HJM: manuscript editing. Y-SC: assistance of animal care and experiment. S-TD: research idea formation and research monitoring. All authors read and approved the final manuscript.
Ethics approval and consent to participate
For this survival study, we used 9 pAdpoR1 mice and 9 WT mice (3 mice for one dose X 3 doses); and for this colitis study, we used 48 pAdpoR1 mice and 48 WT mice (8 mice for one group X 4 groups X 3 independent experiments).
For the all mouse experiments were approved by the Institutional Animal Care and Use Committee, National Taiwan University (Taipei, Taiwan). All experimental procedures were performed in accordance with the protocols and the ethical regulations approved by the Institutional Animal Care and Use Committees of National Taiwan University (Taipei, Taiwan).
Consent for publication
All authors approved the submission of the manuscript for publication.
The authors declare that they have no competing interests.
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- Loftus EV Jr. Clinical epidemiology of inflammatory bowel disease: incidence, prevalence, and environmental influences. Gastroenterology. 2004;126(6):1504–17.View ArticlePubMedGoogle Scholar
- Podolsky DK. Inflammatory bowel disease (2). N Engl J Med. 1991;325(14):1008–16.View ArticlePubMedGoogle Scholar
- Ghione S., Sarter H., Fumery M., Armengol-Debeir L., Savoye G., Ley D., Spyckerelle C., Pariente B., Peyrin-Biroulet L., Turck D., Gower-Rousseau C. and Epimad G. Dramatic increase in incidence of ulcerative colitis and Crohn's disease (1988–2011): a population-based study of French adolescents. Am J Gastroenterol, 2017.Google Scholar
- M'Koma AE. Inflammatory bowel disease: an expanding global health problem. Clin Med Insights Gastroenterol. 2013;6:33–47.View ArticlePubMedPubMed CentralGoogle Scholar
- Kaplan GG. The global burden of IBD: from 2015 to 2025. Nat Rev Gastroenterol Hepatol. 2015;12(12):720–7.View ArticlePubMedGoogle Scholar
- Kappelman MD, Rifas-Shiman SL, Kleinman K, Ollendorf D, Bousvaros A, Grand RJ, Finkelstein JA. The prevalence and geographic distribution of Crohn's disease and ulcerative colitis in the United States. Clin Gastroenterol Hepat. 2007;5(12):1424–9.View ArticleGoogle Scholar
- Podolsky DK. The current future understanding of inflammatory bowel disease. Best Pract Res Clin Gastroenterol. 2002;16(6):933–43.View ArticlePubMedGoogle Scholar
- Castano-Milla C, Chaparro M, Gisbert JP. Systematic review with meta-analysis: the declining risk of colorectal cancer in ulcerative colitis. Aliment Pharmacol Ther. 2014;39(7):645–59.View ArticlePubMedGoogle Scholar
- Eaden JA, Abrams KR, Mayberry JF. The risk of colorectal cancer in ulcerative colitis: a meta-analysis. Gut. 2001;48(4):526–35.View ArticlePubMedPubMed CentralGoogle Scholar
- Jess T, Rungoe C, Peyrin-Biroulet L. Risk of colorectal cancer in patients with ulcerative colitis: a meta-analysis of population-based cohort studies. Clin Gastroenterol Hepatol. 2012;10(6):639–45.View ArticlePubMedGoogle Scholar
- Ruan H, Dong LQ. Adiponectin signaling and function in insulin target tissues. J Mol Cell Biol. 2016;8(2):101–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Swarbrick MM, Havel PJ. Physiological, pharmacological, and nutritional regulation of circulating adiponectin concentrations in humans. Metab Syndr Relat Disord. 2008;6(2):87–102.View ArticlePubMedPubMed CentralGoogle Scholar
- Waki H, Yamauchi T, Kamon J, Kita S, Ito Y, Hada Y, Uchida S, Tsuchida A, Takekawa S, Kadowaki T. Generation of globular fragment of adiponectin by leukocyte elastase secreted by monocytic cell line THP-1. Endocrinology. 2005;146(2):790–6.View ArticlePubMedGoogle Scholar
- Yamauchi T, Kamon J, Ito Y, Tsuchida A, Yokomizo T, Kita S, Sugiyama T, Miyagishi M, Hara K, Tsunoda M, Murakami K, Ohteki T, Uchida S, Takekawa S, Waki H, Tsuno NH, Shibata Y, Terauchi Y, Froguel P, Tobe K, Koyasu S, Taira K, Kitamura T, Shimizu T, Nagai R, Kadowaki T. Cloning of adiponectin receptors that mediate antidiabetic metabolic effects. Nature. 2003;423(6941):762–9.View ArticlePubMedGoogle Scholar
- Fisman EZ, Tenenbaum A. Adiponectin: a manifold therapeutic target for metabolic syndrome, diabetes, and coronary disease? Cardiovasc Diabetol. 2014;13:103.View ArticlePubMedPubMed CentralGoogle Scholar
- Fantuzzi G. Adiponectin and inflammation: consensus and controversy. J Allergy Clin Immunol. 2008;121(2):326–30.View ArticlePubMedGoogle Scholar
- Arsenescu V, Narasimhan ML, Halide T, Bressan RA, Barisione C, Cohen DA, de Villiers WJ, Arsenescu R. Adiponectin and plant-derived mammalian adiponectin homolog exert a protective effect in murine colitis. Dig Dis Sci. 2011;56(10):2818–32.View ArticlePubMedGoogle Scholar
- Nishihara T, Matsuda M, Araki H, Oshima K, Kihara S, Funahashi T, Shimomura I. Effect of adiponectin on murine colitis induced by dextran sulfate sodium. Gastroenterology. 2006;131(3):853–61.View ArticlePubMedGoogle Scholar
- Obeid S, Wankell M, Charrez B, Sternberg J, Kreuter R, Esmaili S, Ramezani-Moghadam M, Devine C, Read S, Bhathal P, Lopata A, Ahlensteil G, Qiao L, George J, Hebbard L. Adiponectin confers protection from acute colitis and restricts a B cell immune response. J Biol Chem. 2017;292(16):6569–82.View ArticlePubMedGoogle Scholar
- Saxena A, Chumanevich A, Fletcher E, Larsen B, Lattwein K, Kaur K, Fayad R. Adiponectin deficiency: role in chronic inflammation induced colon cancer. Biochim Biophys Acta. 2012;1822(4):527–36.View ArticlePubMedGoogle Scholar
- Sideri A, Stavrakis D, Bowe C, Shih DQ, Fleshner P, Arsenescu V, Arsenescu R, Turner JR, Pothoulakis C, Karagiannides I. Effects of obesity on severity of colitis and cytokine expression in mouse mesenteric fat. Potential role of adiponectin receptor 1. Am J Physiol Gastrointest Liver Physiol. 2015;308(7):G591–604.View ArticlePubMedPubMed CentralGoogle Scholar
- Fayad R, Pini M, Sennello JA, Cabay RJ, Chan L, Xu A, Fantuzzi G. Adiponectin deficiency protects mice from chemically induced colonic inflammation. Gastroenterology. 2007;132(2):601–14.View ArticlePubMedGoogle Scholar
- Kaur K, Saxena A, Larsen B, Truman S, Biyani N, Fletcher E, Baliga MS, Ponemone V, Hegde S, Chanda A, Fayad R. Mucus mediated protection against acute colitis in adiponectin deficient mice. J Inflamm. 2015;12:35.View ArticleGoogle Scholar
- Kobayashi SD, DeLeo FR. Role of neutrophils in innate immunity: a systems biology-level approach. Wiley Interdiscip Rev Syst Biol Med. 2009;1(3):309–33.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhou GX, Liu Z. Potential roles of neutrophils in regulating intestinal mucosal inflammation of inflammatory bowel disease. J Dig Dis. 2017;18(9):495-503.Google Scholar
- Wera O, Lancellotti P, Oury C. The dual role of neutrophils in inflammatory bowel diseases. J Clin Med. 201617;5(12):E118. https://doi.org/10.3390/jcm5120118.
- Natsui M, Kawasaki K, Takizawa H, Hayashi SI, Matsuda Y, Sugimura K, Seki K, Narisawa R, Sendo F, Asakura H. Selective depletion of neutrophils by a monoclonal antibody, RP-3, suppresses dextran sulphate sodium-induced colitis in rats. J Gastroenterol Hepatol. 1997;12(12):801–8.View ArticlePubMedGoogle Scholar
- Zhang R, Ito S, Nishio N, Cheng Z, Suzuki H, Isobe K. Up-regulation of Gr1+CD11b+ population in spleen of dextran sulfate sodium administered mice works to repair colitis. Inflamm Allergy Drug Targets. 2011;10(1):39–46.View ArticlePubMedGoogle Scholar
- Fournier BM, Parkos CA. The role of neutrophils during intestinal inflammation. Mucosal Immunol. 2012;5(4):354–66.View ArticlePubMedGoogle Scholar
- Cai L, Xu S, Piao C, Qiu S, Li H, Du J. Adiponectin induces CXCL1 secretion from cancer cells and promotes tumor angiogenesis by inducing stromal fibroblast senescence. Mol Carcinog. 2016;55(11):1796–806.View ArticlePubMedGoogle Scholar
- Kitahara K, Kusunoki N, Kakiuchi T, Suguro T, Kawai S. Adiponectin stimulates IL-8 production by rheumatoid synovial fibroblasts. Biochem Biophys Res Commun. 2009;378(2):218–23.View ArticlePubMedGoogle Scholar
- Wanninger J, Neumeier M, Weigert J, Bauer S, Weiss TS, Schaffler A, Krempl C, Bleyl C, Aslanidis C, Scholmerich J, Buechler C. Adiponectin-stimulated CXCL8 release in primary human hepatocytes is regulated by ERK1/ERK2, p38 MAPK, NF-kappaB, and STAT3 signaling pathways. Am J Physiol Gastrointest Liver Physiol. 2009;297(3):G611–8.View ArticlePubMedGoogle Scholar
- Liu BH, Lin YY, Wang YC, Huang CW, Chen CC, Wu SC, Mersmann HJ, Cheng WT, Ding ST. Porcine adiponectin receptor 1 transgene resists high-fat/sucrose diet-induced weight gain, hepatosteatosis and insulin resistance in mice. Exp Anim. 2013;62(4):347–60.View ArticlePubMedPubMed CentralGoogle Scholar
- Ricciotti E, FitzGerald GA. Prostaglandins and inflammation. Arterioscler Thromb Vasc Biol. 2011;31(5):986–1000.View ArticlePubMedPubMed CentralGoogle Scholar
- Hammond ME, Lapointe GR, Feucht PH, Hilt S, Gallegos CA, Gordon CA, Giedlin MA, Mullenbach G, Tekamp-Olson P. IL-8 induces neutrophil chemotaxis predominantly via type I IL-8 receptors. J Immunol. 1995;155(3):1428–33.PubMedGoogle Scholar
- Yamauchi T, Kamon J, Waki H, Imai Y, Shimozawa N, Hioki K, Uchida S, Ito Y, Takakuwa K, Matsui J, Takata M, Eto K, Terauchi Y, Komeda K, Tsunoda M, Murakami K, Ohnishi Y, Naitoh T, Yamamura K, Ueyama Y, Froguel P, Kimura S, Nagai R, Kadowaki T. Globular adiponectin protected ob/ob mice from diabetes and ApoE-deficient mice from atherosclerosis. J Biol Chem. 2003;278(4):2461–8.View ArticlePubMedGoogle Scholar
- Yamauchi T, Kamon J, Waki H, Terauchi Y, Kubota N, Hara K, Mori Y, Ide T, Murakami K, Tsuboyama-Kasaoka N, Ezaki O, Akanuma Y, Gavrilova O, Vinson C, Reitman ML, Kagechika H, Shudo K, Yoda M, Nakano Y, Tobe K, Nagai R, Kimura S, Tomita M, Froguel P, Kadowaki T. The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity. Nat Med. 2001;7(8):941–6.View ArticlePubMedGoogle Scholar
- Yang WS, Lee WJ, Funahashi T, Tanaka S, Matsuzawa Y, Chao CL, Chen CL, Tai TY, Chuang LM. Weight reduction increases plasma levels of an adipose-derived anti-inflammatory protein, adiponectin. J Clin Endocrinol Metab. 2001;86(8):3815–9.View ArticlePubMedGoogle Scholar
- Comuzzie AG, Funahashi T, Sonnenberg G, Martin LJ, Jacob HJ, Black AE, Maas D, Takahashi M, Kihara S, Tanaka S, Matsuzawa Y, Blangero J, Cohen D, Kissebah A. The genetic basis of plasma variation in adiponectin, a global endophenotype for obesity and the metabolic syndrome. J Clin Endocrinol Metab. 2001;86(9):4321–5.View ArticlePubMedGoogle Scholar
- Hotta K, Funahashi T, Arita Y, Takahashi M, Matsuda M, Okamoto Y, Iwahashi H, Kuriyama H, Ouchi N, Maeda K, Nishida M, Kihara S, Sakai N, Nakajima T, Hasegawa K, Muraguchi M, Ohmoto Y, Nakamura T, Yamashita S, Hanafusa T, Matsuzawa Y. Plasma concentrations of a novel, adipose-specific protein, adiponectin, in type 2 diabetic patients. Arterioscler Thromb Vasc Biol. 2000;20(6):1595–9.View ArticlePubMedGoogle Scholar
- Hu E, Liang P, Spiegelman BM. AdipoQ is a novel adipose-specific gene dysregulated in obesity. J Biol Chem. 1996;271(18):10697–703.View ArticlePubMedGoogle Scholar
- Tsunekawa T, Hayashi T, Suzuki Y, Matsui-Hirai H, Kano H, Fukatsu A, Nomura N, Miyazaki A, Iguchi A. Plasma adiponectin plays an important role in improving insulin resistance with glimepiride in elderly type 2 diabetic subjects. Diabetes Care. 2003;26(2):285–9.View ArticlePubMedGoogle Scholar
- Ding ST, Liu BH, Ko YH. Cloning and expression of porcine adiponectin and adiponectin receptor 1 and 2 genes in pigs. J Anim Sci. 2004;82(11):3162–74.View ArticlePubMedGoogle Scholar
- Tanabe H, Fujii Y, Okada-Iwabu M, Iwabu M, Nakamura Y, Hosaka T, Motoyama K, Ikeda M, Wakiyama M, Terada T, Ohsawa N, Hato M, Ogasawara S, Hino T, Murata T, Iwata S, Hirata K, Kawano Y, Yamamoto M, Kimura-Someya T, Shirouzu M, Yamauchi T, Kadowaki T, Yokoyama S. Crystal structures of the human adiponectin receptors. Nature. 2015;520(7547):312–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Huypens P, Moens K, Heimberg H, Ling Z, Pipeleers D, Van de Casteele M. Adiponectin-mediated stimulation of AMP-activated protein kinase (AMPK) in pancreatic beta cells. Life Sci. 2005;77(11):1273–82.View ArticlePubMedGoogle Scholar
- Chou IP, Chiu YP, Ding ST, Liu BH, Lin YY, Chen CY. Adiponectin receptor 1 overexpression reduces lipid accumulation and hypertrophy in the heart of diet-induced obese mice--possible involvement of oxidative stress and autophagy. Endocr Res. 2014;39(4):173–9.View ArticlePubMedGoogle Scholar
- Lin YY, Chen CY, Lin Y, Chiu YP, Chen CC, Liu BH, Mersmann HJ, Wu SC, Ding ST. Modulation of glucose and lipid metabolism by porcine adiponectin receptor 1-transgenic mesenchymal stromal cells in diet-induced obese mice. Cytotherapy. 2013;15(8):971–8.View ArticlePubMedGoogle Scholar
- Betteridge JD, Armbruster SP, Maydonovitch C, Veerappan GR. Inflammatory bowel disease prevalence by age, gender, race, and geographic location in the U.S. military health care population. Inflamm Bowel Dis. 2013;19(7):1421–7.View ArticlePubMedGoogle Scholar
- Karmiris K, Koutroubakis IE, Xidakis C, Polychronaki M, Voudouri T, Kouroumalis EA. Circulating levels of leptin, adiponectin, resistin, and ghrelin in inflammatory bowel disease. Inflamm Bowel Dis. 2006;12(2):100–5.View ArticlePubMedGoogle Scholar
- Weigert J, Obermeier F, Neumeier M, Wanninger J, Filarsky M, Bauer S, Aslanidis C, Rogler G, Ott C, Schaffler A, Scholmerich J, Buechler C. Circulating levels of chemerin and adiponectin are higher in ulcerative colitis and chemerin is elevated in Crohn's disease. Inflamm Bowel Dis. 2010;16(4):630–7.View ArticlePubMedGoogle Scholar
- Yamamoto K, Kiyohara T, Murayama Y, Kihara S, Okamoto Y, Funahashi T, Ito T, Nezu R, Tsutsui S, Miyagawa JI, Tamura S, Matsuzawa Y, Shimomura I, Shinomura Y. Production of adiponectin, an anti-inflammatory protein, in mesenteric adipose tissue in Crohn's disease. Gut. 2005;54(6):789–96.View ArticlePubMedPubMed CentralGoogle Scholar
- Rodrigues VS, Milanski M, Fagundes JJ, Torsoni AS, Ayrizono ML, Nunez CE, Dias CB, Meirelles LR, Dalal S, Coy CS, Velloso LA, Leal RF. Serum levels and mesenteric fat tissue expression of adiponectin and leptin in patients with Crohn's disease. Clin Exp Immunol. 2012;170(3):358–64.View ArticlePubMedPubMed CentralGoogle Scholar
- Chouliaras G, Panayotou I, Margoni D, Mantzou E, Pervanidou P, Manios Y, Chrousos GP, Roma E. Circulating leptin and adiponectin and their relation to glucose metabolism in children with Crohn's disease and ulcerative colitis. Pediatr Res. 2013;74(4):420–6.View ArticlePubMedGoogle Scholar
- Waluga M, Hartleb M, Boryczka G, Kukla M, Zwirska-Korczala K. Serum adipokines in inflammatory bowel disease. World J Gastroenterol. 2014;20(22):6912–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Izzo RS, Witkon K, Chen AI, Hadjiyane C, Weinstein MI, Pellecchia C. Neutrophil-activating peptide (interleukin-8) in colonic mucosa from patients with Crohn's disease. Scand J Gastroenterol. 1993;28(4):296–300.View ArticlePubMedGoogle Scholar
- Mahida YR, Ceska M, Effenberger F, Kurlak L, Lindley I, Hawkey CJ. Enhanced synthesis of neutrophil-activating peptide-1/interleukin-8 in active ulcerative colitis. Clin Sci. 1992;82(3):273–5.View ArticlePubMedGoogle Scholar
- Mazzucchelli L, Hauser C, Zgraggen K, Wagner H, Hess M, Laissue JA, Mueller C. Expression of interleukin-8 gene in inflammatory bowel disease is related to the histological grade of active inflammation. Am J Pathol. 1994;144(5):997–1007.PubMedPubMed CentralGoogle Scholar
- Mitsuyama K, Toyonaga A, Sasaki E, Watanabe K, Tateishi H, Nishiyama T, Saiki T, Ikeda H, Tsuruta O, Tanikawa K. IL-8 as an important chemoattractant for neutrophils in ulcerative colitis and Crohn's disease. Clin Exp Immunol. 1994;96(3):432–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Puleston J, Cooper M, Murch S, Bid K, Makh S, Ashwood P, Bingham AH, Green H, Moss P, Dhillon A, Morris R, Strobel S, Gelinas R, Pounder RE, Platt A. A distinct subset of chemokines dominates the mucosal chemokine response in inflammatory bowel disease. Aliment Pharmacol Ther. 2005;21(2):109–20.View ArticlePubMedGoogle Scholar
- Raab Y, Gerdin B, Ahlstedt S, Hallgren R. Neutrophil mucosal involvement is accompanied by enhanced local production of interleukin-8 in ulcerative colitis. Gut. 1993;34(9):1203–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Saniabadi AR, Tanaka T, Ohmori T, Sawada K, Yamamoto T, Hanai H. Treating inflammatory bowel disease by adsorptive leucocytapheresis: a desire to treat without drugs. World J Gastroenterol. 2014;20(29):9699–715.View ArticlePubMedPubMed CentralGoogle Scholar
- Brannigan AE, O'Connell PR, Hurley H, O'Neill A, Brady HR, Fitzpatrick JM, Watson RW. Neutrophil apoptosis is delayed in patients with inflammatory bowel disease. Shock. 2000;13(5):361–6.View ArticlePubMedGoogle Scholar
- Ina K, Kusugami K, Hosokawa T, Imada A, Shimizu T, Yamaguchi T, Ohsuga M, Kyokane K, Sakai T, Nishio Y, Yokoyama Y, Ando T. Increased mucosal production of granulocyte colony-stimulating factor is related to a delay in neutrophil apoptosis in inflammatory bowel disease. J Gastroenterol Hepatol. 1999;14(1):46–53.Google Scholar
- Palmen MJ, Dijkstra CD, van der Ende MB, Pena AS, van Rees EP. Anti-CD11b/CD18 antibodies reduce inflammation in acute colitis in rats. Clin Exp Immunol. 1995;101(2):351–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Targan SR, Feagan BG, Fedorak RN, Lashner BA, Panaccione R, Present DH, Spehlmann ME, Rutgeerts PJ, Tulassay Z, Volfova M, Wolf DC, Hernandez C, Bornstein J, Sandborn WJ, International Efficacy of Natalizumab in Crohn's Disease R. and Remission Trial G. Natalizumab for the treatment of active Crohn's disease: results of the ENCORE trial. Gastroenterology. 2007;132(5):1672–83.View ArticlePubMedGoogle Scholar
- Morteau O, Morham SG, Sellon R, Dieleman LA, Langenbach R, Smithies O, Sartor RB. Impaired mucosal defense to acute colonic injury in mice lacking cyclooxygenase-1 or cyclooxygenase-2. J Clin Invest. 2000;105(4):469–78.View ArticlePubMedPubMed CentralGoogle Scholar
- Singer II, Kawka DW, Schloemann S, Tessner T, Riehl T, Stenson WF. Cyclooxygenase 2 is induced in colonic epithelial cells in inflammatory bowel disease. Gastroenterology. 1998;115(2):297–306.View ArticlePubMedGoogle Scholar
- Sturm EM, Radnai B, Jandl K, Stancic A, Parzmair GP, Hogenauer C, Kump P, Wenzl H, Petritsch W, Pieber TR, Schuligoi R, Marsche G, Ferreiros N, Heinemann A, Schicho R. Opposing roles of prostaglandin D2 receptors in ulcerative colitis. J Immunol. 2014;193(2):827–39.View ArticlePubMedPubMed CentralGoogle Scholar
- Chen X, Lu J, Bao J, Guo J, Shi J, Wang Y. Adiponectin: a biomarker for rheumatoid arthritis? Cytokine Growth Factor Rev. 2013;24(1):83–9.View ArticlePubMedGoogle Scholar
- Kusunoki N, Kitahara K, Kojima F, Tanaka N, Kaneko K, Endo H, Suguro T, Kawai S. Adiponectin stimulates prostaglandin E(2) production in rheumatoid arthritis synovial fibroblasts. Arthritis Rheum. 2010;62(6):1641–49.Google Scholar