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Table 7 Overview of CRISPR/Cas9 in virus genome modification

From: CRISPR/Cas9: the Jedi against the dark empire of diseases

Virus Method of delivery of CRISPR/Cas9 Conclusion/outcome Reference
HSV-1 Transfection into HEK293 cells Modification of ICP0 gene in different locations of genome [106]
EBV Nucleofaction into Burkhitt’s lymphoma cell line Complete virus clearance in 25% cells, partial in 50% [87]
EBV Transfection into HEK 293-BX1 and C666–1 cells Loss of BART Micro RNA expression [88]
HPV Lentiviral transduction into HELA and SiHA cell lines Indel mutations in the E6 and E7 genes [90]
HBV Transfection in to Huh cells Cleavage of the HBV genome-expressing template [93]
HBV Hydrodynamic injection into C57BL/6 mice Cleavage of the HBV genome-expressing template [107]
HBV Transfection into HepG2 cell line Fragmentation of viral genome [94]
HBV Lentiviral transduction into HepAD cell line(Chronic HBV infection) Inhibition of viral DNA production [95]
HIV Lentiviral transduction into SupT1 CD4+ T cell line Inactivation of virus and acceleration of virus escape [100]
HIV Lentiviral transduction into T-cells Inhibition of early phase viral infection, but anti-HIV potency was not consistent in multiple rounds [104]
HIV Retro-orbital injection into transgenic mice Decrease of viral gene expression in T-cells [105]
Polyomavirus (JCV) Transfection into TC 620 cell line Inactivation of T-antigen gene [108]