CRISPR/Cas9: the Jedi against the dark empire of diseases

Table 7 Overview of CRISPR/Cas9 in virus genome modification

Virus	Method of delivery of CRISPR/Cas9	Conclusion/outcome	Reference
HSV-1	Transfection into HEK293 cells	Modification of ICP0 gene in different locations of genome	[106]
EBV	Nucleofaction into Burkhitt’s lymphoma cell line	Complete virus clearance in 25% cells, partial in 50%	[87]
EBV	Transfection into HEK 293-BX1 and C666–1 cells	Loss of BART Micro RNA expression	[88]
HPV	Lentiviral transduction into HELA and SiHA cell lines	Indel mutations in the E6 and E7 genes	[90]
HBV	Transfection in to Huh cells	Cleavage of the HBV genome-expressing template	[93]
HBV	Hydrodynamic injection into C57BL/6 mice	Cleavage of the HBV genome-expressing template	[107]
HBV	Transfection into HepG2 cell line	Fragmentation of viral genome	[94]
HBV	Lentiviral transduction into HepAD cell line(Chronic HBV infection)	Inhibition of viral DNA production	[95]
HIV	Lentiviral transduction into SupT1 CD4+ T cell line	Inactivation of virus and acceleration of virus escape	[100]
HIV	Lentiviral transduction into T-cells	Inhibition of early phase viral infection, but anti-HIV potency was not consistent in multiple rounds	[104]
HIV	Retro-orbital injection into transgenic mice	Decrease of viral gene expression in T-cells	[105]
Polyomavirus (JCV)	Transfection into TC 620 cell line	Inactivation of T-antigen gene	[108]

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