Fig. 6

The impact of zTM-b knockdown in neural development. Wild-type embryos, subjected to with/without injecting TM-b MO and rescuing with hTM, were observed and collected at the indicated stages for evaluating the neural tissues development. a, b Embryos at 1 dpf were imaged dorsally under a light dissecting microscope. Those embryos displaying absence or aberrant formation of brain ventricle (arrow) and brain structure (such as mid-hind brain boundary indicated by arrowhead) were categorized as abnormal and quantified. c-e Embryos at 1 dpf were subjected to WISH for the distribution of pax2.1 (a marker of central nervous system) and sox10 (a marker of neural crest cells). Embryos were imaged both laterally (l) and dorsally (d) with anterior to the left. e Embryos displaying signal with altered intensity or distribution pattern for sox10, in comparison to wild-type, were categorized as abnormal and quantified. f, g Larval spontaneous movement was examined by video-recording the embryos at 1 dpf for 5 min and counting for the numbers of moving episode for each embryo. Presented here is a representative image of a series of video stills showing the arrangement of examined embryos. h, i The neuromasts of lateral line (arrows) in embryos of 2 dpf were visualized by staining with 4-Di-2-Asp and imaged laterally with anterior to the left. Those embryos displaying absent or aberrantly distributed neuromasts were categorized as abnormal and quantified. Data were collected from at least 3 independent experiments with the total sample numbers of 18–127 for each group in different experiments. WT, wild-type embryos; MO, zTM-b morphants; hTM, human thrombomodulin; OV, Otic vesicles