Fig. 4

The deposition of serum IgG and purified C1q proteins on Cjr⁺-RS218 and Cjr⁻-RS218. (a) Levels of IgG deposition on the indicated strains after incubation in 40% NHS. (b) Flow cytometry histogram of IgG deposition on the bacteria after 3 h of incubation in 40% NHS. (c) Levels of purified C1q protein on the indicated strains after incubation with a solution containing 32 μg/ml C1q protein for 3 h. (d) Flow cytometry histogram of C1q deposition on the bacteria after incubation with a solution containing 32 μg/ml C1q protein for 3 h. For (a) and (c), the data are derived from flow cytometry analyses and presented as GMFI. The IgG depleted-NHS control group was bacteria incubated in IgG depleted-NHS, while the unstaining control groups were the bacteria without fluorescence staining. *, P value < 0.05