Fig. 5

The survival of Cjr⁻-RS218 and Cjr⁺-RS218 under a similar level of MAC-mediated attack in human serum. (a) Cjr⁻-RS218 and Cjr⁺-RS218 showed a similar level of MAC binding to their surfaces after incubation with 80 and 30% NHS, respectively, for 3 h. The data are derived from flow cytometry analyses and presented as GMFI. The unstaining control group was the bacteria without fluorescence staining. (b) Flow cytometry histogram of MAC deposition on Cjr⁻-RS218 and Cjr⁺-RS218 after 3 h of incubation in 80 and 30% NHS, respectively. (c) The relative survival rates of Cjr⁻-RS218 and Cjr⁺-RS218 after incubation in 80% serum (NHS or HI-NHS) and 30% serum (NHS or HI-NHS), respectively, for 3 h. The data are shown as the relative survival rates compared with those of Cjr⁻-RS218. The results are shown as the means ± standard deviations, and the data are representative of three independent experiments performed in triplicate. *, P value < 0.05