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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: The fciTABC and feoABI systems contribute to ferric citrate acquisition in Stenotrophomonas maltophilia

Fig. 3

Protein–protein interaction assessed by bacterial adenylate cyclase two-hybrid (BACTH) system. Beta-galactosidase activity was determined in E. coli DHM1 strain coexpressing pUT18- and pKT25-derived plasmids. The pUT18- and pKT25-derived plasmids as indicated were co-transformed into E. coli DHM1. The transformants were grown in LB broth with ampicillin, kanamycin and IPTG for 16 h. Beta-galactosidase activity was determined and expressed as Miller units. Black dots represent the results of three independent experiments. Each bar represents the mean value of three independent experiments. *, P < 0.01, significance calculated by Student’s t test. White bars: Void, pUT18 & pKT25; FeoB control, pKT25-FeoB & pUT18; FeoI control, pKT25 & pUT18-FeoI; FeoBt control, pKT25-FeoBt & pUT18; FeoBc control, pKT25-FeoBc & pUT18. Black bars: FeoB-FeoI, pKT25-FeoB & pUT18-FeoI; FeoBt-FeoI, pKT25-FeoBt & pUT18-FeoI; FeoBc-FeoI, pKT25-FeoBc & pUT18-FeoI. Gray bar: RseA-RpoE, pKT25-RseA & pUT18-RpoE (as a positive control)

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Journal of Biomedical Science

ISSN: 1423-0127