Fig. 5

Perturbations of purine and pyrimidine nucleotides. The polymyxin B/rifampicin combination induced significant increases in the levels of a large number of nucleotides at i 0.25 h, ii 1 h and iii 4 h. There were no significant changes in nucleotide levels at 24 h following treatment with either monotherapy or the combination. No treatment (CTRL); PB polymyxin B, RIF rifampicin, COMBO polymyxin B/rifampicin combination. Significantly changed metabolites were identified with > 1.0 and < − 1.0 log₂FC and FDR ≤ 0.05