- Open Access
Identification of 11-amino acid peptides that disrupt Notch-mediated processes in Drosophila
- Haiwei Pi†1Email author,
- Yi-Chun Huang†1,
- I-Chun Chen1,
- Chung-De Lin1,
- Hsiao-Fong Yeh1 and
- Li-Mei Pai2
© Pi et al; licensee BioMed Central Ltd. 2011
Received: 11 March 2011
Accepted: 17 June 2011
Published: 17 June 2011
The conserved Notch signaling pathway regulates cell fate decisions and maintains stem cells in multicellular organisms. Up-regulation of Notch signaling is observed in several types of cancer and is causally involved in proliferation and survival of cancer cells. Thus, it is of great interest to look for anti-Notch reagents for therapeutic purposes. In model animal Drosophila, Notch signaling restricts selection of sensory organ precursors (SOPs) during external sensory (ES) organ development. To look for novel genes that can suppress Notch signaling, we performed a gain-of-function modifier screen to look for genes that enhance the phenotype of ectopic ES organs induced by overexpression of phyllopod, a gene required for SOP specification.
From the gain-of-function screen, we discovered that overexpression of polished rice/tarsal-less (pri/tal) increases the numbers of ES organs as well as SOPs. pri/tal is a polycistronic gene that contains four short open reading frames encoding three 11-amino acid and one 32-amino acid peptides. Ectopic expression of the 11 amino-acid peptides recapitulates the pri/tal misexpression phenotype in ectopic ES organ formation. In situ hybridization experiment reveals that pri/tal mRNA is expressed in the SOPs of the chemosensory organs and the stretch-sensing chordotonal organs.
In Drosophila wing development, the Notch signaling pathway mediates the formation of the dorsal-ventral (DV) compartmental boundary and the restriction of the vein width from the primordial veins, the proveins. We also found that pri/tal mRNA is expressed in the DV boundary and the longitudinal proveins, and overexpression of Pri/Tal peptides disrupts the DV boundary formation and helps to expand the width of the wing vein. Genetic analyses further show that a Notch loss-of-function allele strongly enhances these two phenotypes. Cut and E(spl)mβ are target genes of the Notch pathway in DV boundary formation and vein specification, respectively. We also found that overexpression of Pri/Tal peptides abolishes Cut expression and co-expression of Pri/Tal peptides with phyl strongly reduces E(spl)mβ expression.
We show for the first time that the overexpression of Pri/Tal 11-amino acid peptides disrupts multiple Notch-mediated processes and reduces Notch target gene expression in Drosophila, suggesting that these peptides have novel antagonistic activity to the Notch pathway. Thus, our discovery might provide insights into designing new therapeutic reagents for Notch-related diseases.
The Notch pathway is an evolutionally conserved signaling system required in a wide range of developmental processes and the maintenance of stem cells [1–3]. Malignancies including T-cell acute lymphoblastic leukemia , breast cancer , pancreatic cancer , lung cancer  and ovarian cancer  are associated with up-regulation of the Notch signaling activity. Inhibition of Notch signaling pathway has been shown to deplete stem-like cells and suppress the tumor-forming activity in brain tumors , and suppress proliferation and induce apoptosis of ovarian and lung cancer cells [7, 8].
One excellent model to study the Notch signaling pathway is the development of the fruit fly Drosophila melanogaster. During Drosophila development, the Notch pathway is involved in developmental processes such as the selection of neural precursors and the specification of wing veins and wing margins [2, 10, 11]. The Drosophila wing veins are formed with a prominent and invariant pattern in adult wings. During larval development, formation of longitudinal vein is initiated by the specification of proveins in the wing imaginal discs. Further restriction of the provein width from eight or nine-cells to two or three-cells requires the activation of the Notch pathway during the pupal stage. In lateral provein cells, the activation of receptor Notch (N) by its ligand Delta expressed in the central region leads to the suppression of vein cell differentiation [12, 13]. In N loss-of-function mutants, lateral provein cells differentiate into vein fate, causing the widening of wing veins .
The Notch pathway is also required to define the dorsal-ventral (DV) compartmental boundary of the wings. Transduction of the Notch pathway at the DV boundary activates downstream targets such as genes encoding the signal molecule Wingless (Wg) and the homeodomain transcription factor Cut [15–17]. When N, wg or cut activity at the DV boundary is disrupted, notched adult wings are detected along the margin.
One classical model to study the role of the Notch pathway in neurogenesis is the development of Drosophila sensory organs. Sensory organ development is initiated by basic-helix-loop-helix (bHLH) proneural proteins that are first expressed in neural-competent proneural clusters of cells, in which each cell in the cluster is endowed with the potential to become the sensory organ precursor (SOP) [18, 19]. The expression of bHLH proneural proteins in proneural clusters is further restricted to and refined in single cells, the future SOPs, through the Notch pathway [11, 20]. In mutants with reduced Notch signaling activity, ectopic SOPs are specified within the proneural clusters or the proneural stripes, leading to the generation of ectopic SOPs and an increase in the ES organ density [21–24].
In order to identify novel genes regulating SOP specification and potentially the Notch pathway, we performed a gain-of-function screen to identify genes that modify the phenotype of ectopic external sensory (ES) organs induced by overexpression of phyllopod (phyl). The phyl gene is a direct downstream target of proneural proteins and is required for SOP specification and ES organ formation [25, 26]. Among the six modifiers identified in the screen, we focused on gene polished rice/tarsal-less (pri/tal). pri/tal enhances the phyl- overexpression phenotype in the formation of ectopic ES organs. It encodes a polycistronic mRNA that contains four short open reading frams (ORFs) of 36 and 99 base pairs in length (Additional File 1, Figure S1). These four short ORFs express four peptides (Pep1-4). Pep1, Pep2, and Pep3 have only 11 amino acids with Pep1 and Pep2 being identical. Pep4 has 32 amino acids. All four peptides share the core motif of LDPTGQ(T)Y that is present twice in Pep4 [27, 28]. These four peptides function redundantly in embryogenesis. pri/tal regulates the formation of dentical belts and tracheal system in embryos [27, 29], and is required for developmental patterning of the legs [28, 30]. In this study, we found that overexpression of Pri/Tal peptides causes ectopic ES organ formation, wing vein expansion, and defects in formation of the compartmental boundary of wing imaginal discs. These phenotypes are similar to those seen in Notch loss-of-function mutants [14, 24]. We further demonstrated that overexpression of Pri/Tal peptides abolishes or helps to reduces the expression of Cut and E(spl)mβ, Notch target genes in mediating DV boundary formation and suppression of vein specification, respectively. Thus, our gain-of-function screen in model animal Drosophila reveals the potential activity of Pri/Tal peptides as peptide antagonists of Notch signaling.
All flies were incubated at 25°C unless indicated otherwise. The following flies were used: UAS-myc-phyl, UAS-pri/tal (this study), UAS-pep1, UAS-pep3, and UAS-pri/tal 1-4FS , N 55e11 , UAS-N, E(spl)mβ-lacZ, Eq-Gal4, dpp-Gal4, ato 1 and Df(3R)p 13 , sc 10-1 , and cbl F165 . The Pep1-misexpression mitotic clones were generated in hs-Flp/+; actin > Y+ > Gal4 UAS-GFP/UAS-pep1 larvae. For the EP gain-of-function screen, crosses were set up with males from 1075 independent EP lines and females of Eq-Gal4 UAS-phyl/TM6B. Among them, 273 lines were obtained from the Berkeley Drosophila genome projects. The other 802 newly generated lines were provided by Dr. Cheng-Ting Chien.
In situ RNA hybridization
The same protocal described by Tautz and Pfeifle was used for in situ hybridization . The full-length cDNA (from transcription start +1 to +1532) from EST clone LD11162 was used as the template to generate the pri/tal anti-sense probe.
Immunohistology and X-gal staining
For immunohistology, dissected discs or pupal nota were fixed in 4% formaldehyde or 4% freshly prepared paraformaldehyde for 15 minutes. After washing with 1X PBT (phosphate buffered saline with 0.1% TX-100), discs were incubated with anti-Cut (1:1000) or anti-Hnt (1:25) antibodies (Hybridoma Bank) followed by a Cy3-conjugated secondary antibody. For X-gal staining, dissected discs were fixed in 0.3% glutaraldehyde for 10 minutes. After washing in 1XPBS for 3 minutes, the discs were incubated in an X-gal buffer for 30 minutes at 37°C.
The protein levels of Cut were quantified by measuring the immunofluorescence intensity of anti-Cut antibody staining. All samples were imaged in a single plane and images were analyzed using ImageJ software. To determine the mean fluorescence intensity (FI) of ectopically expressed Cut (FIectopic) within the misexpression clones, 15 Cut- and GFP-positive cells that are adjacent or close to the DV boundary were randomly selected for measurement of the Cy3 intensity. In the same disc, the Cy3 intensity of 15 Cut-positive and GFP-negative cells at the DV boundary and 15 Cut- and GFP-negative cells flanking the DV boundary was measured as the mean endogenous (FIendo) and background (FIback) intensity, respectively. The relative FI was calculated as [(FIectopic- FIback)/( FIendo- FIback)]×100%. For three co-expression clones of Pep1 and N in which the ectopic Cut expression was barely detected, 15 GFP-positive cells flanking the DV boundary were randomly selected for fluorescence measurement.
Identifying candidate genes in ES organ development using an EP overexpression screen
EP screen to look for genes involved in ES organ development.
Gene located downstream of EP insertion
ES organ missing
ES organ missing
ES organ missing
ES organ missing
ES organ missing
ES organ missing
ES organ density increased
ES organ density increased
disorganized ES organ pattern
disorganized ES organ pattern
ES organ density increased
The overexpression of Pri/Tal peptides induces ES organ formation
This study focused on EPC05-660 as it exhibited a strong synergistic interaction with phyl in ES organ formation. While the expression of EPC05-660 alone by Eq-Gal4 induced almost no ectopic ES organs (Figure 1C), coexpression of UAS-phyl and EPC05-660 induced more ES organs than the expression of UAS-phyl alone (Figure 1D). EPC05-660 was inserted 191 base pairs upstream of the transcription start site of pri/tal. To examine whether the ectopic ES organ formation resulted from the expression of pri/tal, a pri/tal cDNA transgene under the control of UAS was generated, which, presumably, produce all four small Pri/Tal peptides. Coexpression of UAS-pri/tal and UAS-phyl by Eq-Gal4 also induced many ectopic ES organs on the notum (Figure 1E), a phenotype similar to the coexpression of EPC05-660 and phyl.
To quantify the numbers of ectopic ES organs, dpp-Gal4 was used to drive pri/tal and phyl expression at the scutellum where only four ES organs are present in wild-type (Figure 1F). The quantification results are shown in Figure 1Q. The transgene UAS-pri/tal 1-4FS , which carries frame-shift mutations in the coding sequences of all four small peptides, was used as a control . While the expression of pri/tal or phyl alone induced a few ectopic ES organs (2.3 ± 0.7 and 1 ± 1.3, respectively, Figure 1G and 1J), coexpression of pri/tal and phyl by dpp-Gal4 induced 7.4 ± 1.5 ectopic ES organs (Figure 1K). The expression of UAS-pri/tal 1-4FS by dpp-Gal4 produced no ectopic ES organs (0 ± 0, Figure 1H) and failed to enhance the phyl-overexpression phenotype (1.5 ± 0.8, Figure 1L).
We next investigated whether the expression of single Pri/Tal peptides is sufficient to synergize with phyl to induce ES organs. Pep1 or Pep3, when coexpressed with Phyl, induced as many as 7.2 ± 1.4 ectopic ES organs with Pep1 and 9.6 ± 2.5 organs with Pep3 (Figure 1M and data not shown). Only a few ectopic ES organs were detected in flies expressing either Pep1 (2.6 ± 0.7) or Pep3 (3.3 ± 1.3) alone (Figure 1I and data not shown). While the analysis using dpp-Gal4 suggested a synergistic interaction between single Pri/Tal peptides and Phyl, it also indicated that Pri/Tal peptides alone are sufficient to promote ES organ formation in the absence of Phyl coexpression.
Each ES organ is composed of four cells (neuron, sheath cell, shaft cell and socket cell) which are derived from asymmetric cell division of a single SOP. The ability of Pep1 and Pep3 to induce ectopic ES organs suggests that Pri/Tal peptides are sufficient to promote formation of the SOPs. By using antibody recognizing Hindsight (Hnt) protein , a marker for SOPs and the SOP progenies, four Hnt-positive clusters were observed at the wild-type pupal scutellum at 12-16 hr APF (after puparium formation) (arrows in Figure 1N). These four clusters of cells correspond to the SOP progenies of the four endogenous scutellar ES organs. In pupae expressing pri/tal through dpp-Gal4, more than four Hnt-positive clusters were observed (arrows in Figure 1P), indicating the formation of ectopic SOPs. No ectopic Hnt-positive cluster was found at the scutellum of dpp-Gal4 > UAS-pri/tal 1-4FS pupae (arrows in Figure 1O). Thus, ectopic SOP formation can be induced by overexpression of Pri/Tal peptides.
Expression patterns of pri/tal in imaginal discs
In larval leg discs, pri/tal is expressed in the tarsal region, and is required for specification of tarsal segments . At 4-8 hr APF, pri/tal mRNA was concentrated at the presumptive joints between tarsal segments (Figure 2E). In wing discs of third instar larvae, pri/tal mRNA was present at the DV compartmental boundary (arrowheads in Figure 2F) and in two stripes of cells straddling the anterior part of the DV boundary (arrows). The latter expression pattern suggests that pri/tal is expressed in the precursors for chemosensory organs. The formation of chemosensory organs along the anterior wing margin depends on the activities of the proneural genes ac and sc. In the sc 10-1 mutant that abolishes both ac and sc activities, pri/tal mRNA expression in these two anterior stripes was abolished (white arrows in Figure 2G). From the late third instar larval to the early pupal stages, pri/tal mRNA was also detected in the provein cells that develop into longitudinal wing veins L2-L5 (Figure 2H and data not shown).
Overexpression of Pri/Tai peptides promotes vein cell fate
During wing vein development, Notch signaling, that refines provein to vein regions, is activated at both the anterior and posterior boundaries of proveins. This is seen by the expression of the Notch pathway target reporter E(spl)mβ-lacZ (arrows in Figure 3I). The expression of phyl or pri/tal alone by dpp-Gal4 did not alter this E(spl)mβ-lacZ pattern (Figure 3J and data not shown). In the coexpression of pri/tal and phyl, however, the lacZ expression at the posterior L3 provein boundary was severely disrupted (white arrow for posterior boundary in Figure 3K). Similar results were observed in the coexpression of Phyl and Pep1 (data not shown). In the control wing discs in which phyl was coexpressed with pri/tal 1-4FS , the lacZ patterns and levels remained indistinguishable from the wild-type discs (Figure 3L).
To test whether expression of the Pri/Tal peptides also affects formation of the other veins, ectopic Pep1-expressing GFP-marked clones under the actin promoter were generated (see Methods). In ten clones that overlapped wing veins, as visualized by GFP expression (Figure 3N'), three displayed vein expansion phenotype (Figure 3N). In many more clones that did not overlap wing veins, no ectopic vein was observed, suggesting that Pep1 alone is sufficient to promote vein fate from the provein cells.
Overexpression of Pri/Tal peptides disrupts DV boundary formation
As shown in Figure 2F, pri/tal was also expressed at the DV boundary of the wing discs, the site of the future wing margin. Expression of pri/tal or Pep1, but not pri/tal 1-4FS , strongly enhanced the wing-notching phenotype of N 55e11 /+ wing (Figure 4A-D). The homeodomain protein Cut, required for the formation of the wing margin , is expressed at the DV boundary in response to activation of the Notch pathway. Thus, overexpression clones of Pep1 were generated to examine whether Cut expression was affected by Pep1. In all Pep1 clones overlapping the DV boundary, Cut expression was strongly inhibited (100%, N = 11) (Figure 4F and 4F'). Similar results were also found in overexpression clones of pri/tal (data not shown). In contrast, no reduction of Cut levels was observed in clones expressing pri/tal 1-4FS (100%, N = 20) (Figure 4E and 4E'). In addition to suppression of Cut levels by the Pep1 within the clones, we found that Cut level was also significantly reduced in non-clonal cells near the clone (white arrow in Figure 4F'). By analyzing Cut levels in twenty-two non-clonal areas that are flanking the clones, we found that Cut expression was strongly suppressed in thirteen of them (59%): ten showed Cut reduction within ten cells from the GFP-positive clones, and three showed Cut reduction in cells that were as far as 10 to 12 cells away from the clone. Thus, Pri/Tal peptides can repress Cut expression at the DV boundary both autonomously and non-autonomously.
Pep1 was further investigated to determine if it could indeed suppress Cut expression induced by the Notch pathway. Overexpression of receptor N together with the non-functional pri/tal 1-4FS autonomously induced ectopic Cut expression in cells adjacent to the DV boundary (yellow arrow in Figure 4G'), similar to that of misexpression of N alone . However, both ectopic and endogenous Cut expressions in N overexpression clones were repressed by the coexpressed Pep1 (Figure 4H and H'). By quantifying the intensity of the anti-Cut antibody staining (see Methods), we found that the average protein level of ectopically expressed Cut induced by the coexpression of N and pri/tal 1-4FS was 85% of the endogenous Cut at the DV boundary (N = 10). In the coexpression clones of N and Pep1, the average protein level of ectopically expressed Cut was reduced to 23% of the endogenous level (N = 11). At times, it was below 5% (N = 3), which meant that it was barely detectable by immunofluorescent staining (yellow arrow within the clone in Figure 4H'). In the coexpression clones of N and Pep1, the non-autonomous suppression of Cut expression at the DV boundary was also observed in eight out of the seventeen non-clonal areas that flanking the GFP-positive clones (47%) (white arrow in Figure 4H'). As the co-expression result shown in Figure 4H, the Pri/Tal peptide is able to repress Cut expression induced by the activation of the Notch pathway.
The Notch pathway is a highly conserved signaling system that regulates cell proliferation, differentiation and death, and aberrant activation of Notch signaling is associated with several types of tumor . Although γ-secretase inhibitors have been shown to successfully suppress tumor growth in several types of Notch-related cancers [48–51], the cytotoxicity in the GI tract complicates its potential in cancer therapy . Thus, it is important to look for more anti-Notch reagents for therapeutic purposes.
During Drosophila development, the Notch pathway is involved in the developmental processes such as selection of neural precursors and specification of wing veins and wing margins [2, 10, 11]. Overexpression of Pri/Tal peptides leads to phenotypes such as the formation of ectopic SOPs, expansion of the wing veins and disruption of the DV boundary. These are reminiscent of the N loss-of-function phenotypes. Overexpression of pri/tal or Pep1 eliminates or helps to reduce the expression of Notch target genes Cut and E(spl)mβ. Furthermore, expression of Pep1 represses Cut expression induced by the activation of the Notch pathway. Pri/Tal peptides mediate F-actin assembly in the embryonic tracheal development . The effect of Pri/Tal peptides on cytoskeletal dynamics and how it might contribute to the suppression of the Notch-mediated expression of Cut and E(spl)mβ needs further study.
During wing vein development, EGFR and Notch signaling pathways act antagonistically in specifying longitudinal veins . The E3 ligase encoded by cbl negatively regulates EGFR signaling by downregulating the level of active EGF receptors . Although pri/tal acts synergistically with N 55e11 to cause wing vein expansion, vein width was not further modified when one copy of cbl null allele (cbl F165 ) was introduced into dpp-Gal4 > UAS-pri/tal flies (Additional File 3, Figure S3). Thus, the genetic interaction results suggest a more specific effect of pri/tal to the Notch signaling than to the EGFR pathway in vein fate specification.
A previous report showed that Pri/Tal peptides could function non-autonomously to regulate formation of the larval ventral denticle and adult tarsal segment [27, 30]. In developing legs, analyses of tal mutant clones show that Tal/Pri-dependent signal is local with a range of 2-3 cells . Our analysis found that Pri/Tal peptides, when overexpressed, can function as far as 12 cells away from the clones to suppress Cut expression at the DV boundary. The difference in the range of non-autonomy might be due to the different expression levels. Alternatively, the extracellular distribution of Pri/Tal peptides might be differentially regulated in different developmental processes, or different peptide concentrations are required for eliciting distinct signaling responses, such as development of leg tarsal segments and suppression of Cut expression at the DV boundary. We also found that the reduction of Cut levels in cells within the clones is stronger than that in cells outside the clone and the severity of the reduction is conversely correlated with the distance from the clones. Therefore, the non-autonomy of Pri/Tal peptides is similar to that of morphogenes, raising the possibility that these extremely small peptides might pass through membranes to exert their non-autonomous effects on neighboring cells.
In the coexpression clones of Pep1 and N, Pep1 also non-autonomously suppressed Cut expression at the developing wing margin in 47% of the non-clonal areas flanking the clones. The non-autonomous suppression effect can be as far as 9 cells from the clones (data not shown). Thus, the presence of N appears not to significantly interfere with the non-autonomous effect of Pep1 to suppress Cut expression.
Although we have shown that overexpression of Pri/Tal peptides, in combination with phyl-coexpression or in N 55e11 /+ heterozygous background, strongly inhibit Notch-mediated developmental processes in SOP selection and wing development, expression of Pri/Tal peptides alone in wild-type flies only have mild effects on these processes. Thus, decoding the molecular mechanisms of Pri/Tal peptides to the Notch pathway is critically important in the future in order to improve its efficiency as potential anti-Notch reagent.
By using Drosophila development as a model system, we identify and demonstrate for the first time that overexpression of Pri/Tal 11-amino acid peptides promotes SOP and wing vein specification, and suppresses DV boundary formation of the developing wing. These are reminiscent of Notch loss-of-function phenotypes. We also found that overexpression of Pri/Tal peptides abolishes or help to reduce the expression of Notch target genes, suggesting that these small peptides have novel activity to negatively modulate the Notch signaling pathway. Thus, our discovery might provide insights into designing new therapeutic reagents for treating Notch-related malignancies and diseases.
We thank Dr. C.-T Chien and Dr. BC Tan for the critical reading of this manuscript, and Dr. Y. Kageyama, Bloomington Stock Center and Developmental Studies Hybridoma Bank for providing fly stocks and reagents. We are grateful for P.-Y. Wang and all the members of Pi's laboratory for discussion and technical support.
- Bray SJ: Notch signalling: a simple pathway becomes complex. Nat Rev Mol Cell Biol. 2006, 7: 678-689. 10.1038/nrm2009.View ArticlePubMedGoogle Scholar
- Artavanis-Tsakonas S, Matsuno K, Fortini ME: Notch signaling. Science. 1995, 268: 225-232. 10.1126/science.7716513.View ArticlePubMedGoogle Scholar
- Chiba S: Notch signaling in stem cell systems. Stem Cells. 2006, 24: 2437-2447. 10.1634/stemcells.2005-0661.View ArticlePubMedGoogle Scholar
- Weng AP, Ferrando AA, Lee W, Morris JPt, Silverman LB, Sanchez-Irizarry C, Blacklow SC, Look AT, Aster JC: Activating mutations of NOTCH1 in human T cell acute lymphoblastic leukemia. Science. 2004, 306: 269-271. 10.1126/science.1102160.View ArticlePubMedGoogle Scholar
- Uyttendaele H, Marazzi G, Wu G, Yan Q, Sassoon D, Kitajewski J: Notch4/int-3, a mammary proto-oncogene, is an endothelial cell-specific mammalian Notch gene. Development. 1996, 122: 2251-2259.PubMedGoogle Scholar
- Miyamoto Y, Maitra A, Ghosh B, Zechner U, Argani P, Iacobuzio-Donahue CA, Sriuranpong V, Iso T, Meszoely IM, Wolfe MS: Notch mediates TGF alpha-induced changes in epithelial differentiation during pancreatic tumorigenesis. Cancer Cell. 2003, 3: 565-576. 10.1016/S1535-6108(03)00140-5.View ArticlePubMedGoogle Scholar
- Konishi J, Kawaguchi KS, Vo H, Haruki N, Gonzalez A, Carbone DP, Dang TP: Gamma-secretase inhibitor prevents Notch3 activation and reduces proliferation in human lung cancers. Cancer Res. 2007, 67: 8051-8057. 10.1158/0008-5472.CAN-07-1022.View ArticlePubMedGoogle Scholar
- Park JT, Li M, Nakayama K, Mao TL, Davidson B, Zhang Z, Kurman RJ, Eberhart CG, Shih Ie M, Wang TL: Notch3 gene amplification in ovarian cancer. Cancer Res. 2006, 66: 6312-6318. 10.1158/0008-5472.CAN-05-3610.View ArticlePubMedGoogle Scholar
- Fan X, Matsui W, Khaki L, Stearns D, Chun J, Li YM, Eberhart CG: Notch pathway inhibition depletes stem-like cells and blocks engraftment in embryonal brain tumors. Cancer Res. 2006, 66: 7445-7452. 10.1158/0008-5472.CAN-06-0858.View ArticlePubMedGoogle Scholar
- Bray S: Notch signalling in Drosophila: three ways to use a pathway. Semin Cell Dev Biol. 1998, 9: 591-597. 10.1006/scdb.1998.0262.View ArticlePubMedGoogle Scholar
- Artavanis-Tsakonas S, Rand MD, Lake RJ: Notch signaling: cell fate control and signal integration in development. Science. 1999, 284: 770-776. 10.1126/science.284.5415.770.View ArticlePubMedGoogle Scholar
- de Celis JF, Bray S, Garcia-Bellido A: Notch signalling regulates veinlet expression and establishes boundaries between veins and interveins in the Drosophila wing. Development. 1997, 124: 1919-1928.PubMedGoogle Scholar
- Huppert SS, Jacobsen TL, Muskavitch MA: Feedback regulation is central to Delta-Notch signalling required for Drosophila wing vein morphogenesis. Development. 1997, 124: 3283-3291.PubMedGoogle Scholar
- de Celis JF, Garcia-Bellido A: Roles of the Notch gene in Drosophila wing morphogenesis. Mech Dev. 1994, 46: 109-122. 10.1016/0925-4773(94)90080-9.View ArticlePubMedGoogle Scholar
- de Celis JF, Garcia-Bellido A, Bray SJ: Activation and function of Notch at the dorsal-ventral boundary of the wing imaginal disc. Development. 1996, 122: 359-369.PubMedGoogle Scholar
- Neumann CJ, Cohen SM: A hierarchy of cross-regulation involving Notch, wingless, vestigial and cut organizes the dorsal/ventral axis of the Drosophila wing. Development. 1996, 122: 3477-3485.PubMedGoogle Scholar
- Micchelli CA, Rulifson EJ, Blair SS: The function and regulation of cut expression on the wing margin of Drosophila: Notch, Wingless and a dominant negative role for Delta and Serrate. Development. 1997, 124: 1485-1495.PubMedGoogle Scholar
- Jan YN, Jan LY: Genetic control of cell fate specification in Drosophila peripheral nervous system. Annu Rev Genet. 1994, 28: 373-393. 10.1146/annurev.ge.28.120194.002105.View ArticlePubMedGoogle Scholar
- Campuzano S, Modolell J: Patterning of the Drosophila nervous system: the achaete-scute gene complex. Trends Genet. 1992, 8: 202-208.View ArticlePubMedGoogle Scholar
- Greenwald I: LIN-12/Notch signaling: lessons from worms and flies. Genes Dev. 1998, 12: 1751-1762. 10.1101/gad.12.12.1751.View ArticlePubMedGoogle Scholar
- Skeath JB, Carroll SB: Regulation of achaete-scute gene expression and sensory organ pattern formation in the Drosophila wing. Genes Dev. 1991, 5: 984-995. 10.1101/gad.5.6.984.View ArticlePubMedGoogle Scholar
- Pi H, Chien CT: Getting the edge: neural precursor selection. J Biomed Sci. 2007, 14: 467-473. 10.1007/s11373-007-9156-4.View ArticlePubMedGoogle Scholar
- Heitzler P, Bourouis M, Ruel L, Carteret C, Simpson P: Genes of the Enhancer of split and achaete-scute complexes are required for a regulatory loop between Notch and Delta during lateral signalling in Drosophila. Development. 1996, 122: 161-171.PubMedGoogle Scholar
- Hartenstein V, Posakony JW: A dual function of the Notch gene in Drosophila sensillum development. Dev Biol. 1990, 142: 13-30. 10.1016/0012-1606(90)90147-B.View ArticlePubMedGoogle Scholar
- Pi H, Wu HJ, Chien CT: A dual function of phyllopod in Drosophila external sensory organ development: cell fate specification of sensory organ precursor and its progeny. Development. 2001, 128: 2699-2710.PubMedGoogle Scholar
- Pi H, Huang SK, Tang CY, Sun YH, Chien CT: phyllopod is a target gene of proneural proteins in Drosophila external sensory organ development. Proc Natl Acad Sci USA. 2004, 101: 8378-8383. 10.1073/pnas.0306010101.PubMed CentralView ArticlePubMedGoogle Scholar
- Kondo T, Hashimoto Y, Kato K, Inagaki S, Hayashi S, Kageyama Y: Small peptide regulators of actin-based cell morphogenesis encoded by a polycistronic mRNA. Nat Cell Biol. 2007, 9: 660-665. 10.1038/ncb1595.View ArticlePubMedGoogle Scholar
- Galindo MI, Pueyo JI, Fouix S, Bishop SA, Couso JP: Peptides encoded by short ORFs control development and define a new eukaryotic gene family. PLoS Biol. 2007, 5: e106-10.1371/journal.pbio.0050106.PubMed CentralView ArticlePubMedGoogle Scholar
- Kondo T, Plaza S, Zanet J, Benrabah E, Valenti P, Hashimoto Y, Kobayashi S, Payre F, Kageyama Y: Small peptides switch the transcriptional activity of Shavenbaby during Drosophila embryogenesis. Science. 2010, 329: 336-339. 10.1126/science.1188158.View ArticlePubMedGoogle Scholar
- Pueyo JI, Couso JP: The 11-aminoacid long Tarsal-less peptides trigger a cell signal in Drosophila leg development. Dev Biol. 2008, 324: 192-201. 10.1016/j.ydbio.2008.08.025.View ArticlePubMedGoogle Scholar
- Sasamura T, Sasaki N, Miyashita F, Nakao S, Ishikawa HO, Ito M, Kitagawa M, Harigaya K, Spana E, Bilder D: neurotic, a novel maternal neurogenic gene, encodes an O-fucosyltransferase that is essential for Notch-Delta interactions. Development. 2003, 130: 4785-4795. 10.1242/dev.00679.View ArticlePubMedGoogle Scholar
- Masucci JD, Miltenberger RJ, Hoffmann FM: Pattern-specific expression of the Drosophila decapentaplegic gene in imaginal disks is regulated by 3' cis-regulatory elements. Genes Dev. 1990, 4: 2011-2023. 10.1101/gad.4.11.2011.View ArticlePubMedGoogle Scholar
- Jarman AP, Grell EH, Ackerman L, Jan LY, Jan YN: Atonal is the proneural gene for Drosophila photoreceptors. Nature. 1994, 369: 398-400. 10.1038/369398a0.View ArticlePubMedGoogle Scholar
- Villares R, Cabrera CV: The achaete-scute gene complex of D. melanogaster: conserved domains in a subset of genes required for neurogenesis and their homology to myc. Cell. 1987, 50: 415-424. 10.1016/0092-8674(87)90495-8.View ArticlePubMedGoogle Scholar
- Pai LM, Barcelo G, Schupbach T: D-cbl, a negative regulator of the Egfr pathway, is required for dorsoventral patterning in Drosophila oogenesis. Cell. 2000, 103: 51-61. 10.1016/S0092-8674(00)00104-5.View ArticlePubMedGoogle Scholar
- Tautz D, Pfeifle C: A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback. Chromosoma. 1989, 98: 81-85. 10.1007/BF00291041.View ArticlePubMedGoogle Scholar
- Rorth P, Szabo K, Bailey A, Laverty T, Rehm J, Rubin GM, Weigmann K, Milan M, Benes V, Ansorge W, Cohen SM: Systematic gain-of-function genetics in Drosophila. Development. 1998, 125: 1049-1057.PubMedGoogle Scholar
- Escudero LM, Caminero E, Schulze KL, Bellen HJ, Modolell J: Charlatan, a Zn-finger transcription factor, establishes a novel level of regulation of the proneural achaete/scute genes of Drosophila. Development. 2005, 132: 1211-1222. 10.1242/dev.01691.View ArticlePubMedGoogle Scholar
- Bailey AM, Posakony JW: Suppressor of hairless directly activates transcription of enhancer of split complex genes in response to Notch receptor activity. Genes Dev. 1995, 9: 2609-2622. 10.1101/gad.9.21.2609.View ArticlePubMedGoogle Scholar
- Lecourtois M, Schweisguth F: The neurogenic suppressor of hairless DNA-binding protein mediates the transcriptional activation of the enhancer of split complex genes triggered by Notch signaling. Genes Dev. 1995, 9: 2598-2608. 10.1101/gad.9.21.2598.View ArticlePubMedGoogle Scholar
- Pickup AT, Lamka ML, Sun Q, Yip ML, Lipshitz HD: Control of photoreceptor cell morphology, planar polarity and epithelial integrity during Drosophila eye development. Development. 2002, 129: 2247-2258.PubMedGoogle Scholar
- Jarman AP, Grau Y, Jan LY, Jan YN: atonal is a proneural gene that directs chordotonal organ formation in the Drosophila peripheral nervous system. Cell. 1993, 73: 1307-1321. 10.1016/0092-8674(93)90358-W.View ArticlePubMedGoogle Scholar
- Milan M, Weihe U, Tiong S, Bender W, Cohen SM: msh specifies dorsal cell fate in the Drosophila wing. Development. 2001, 128: 3263-3268.PubMedGoogle Scholar
- Duffy JB: GAL4 system in Drosophila: a fly geneticist's Swiss army knife. Genesis. 2002, 34: 1-15. 10.1002/gene.10150.View ArticlePubMedGoogle Scholar
- Nagaraj R, Banerjee U: Regulation of Notch and Wingless signalling by phyllopod, a transcriptional target of the EGFR pathway. EMBO J. 2009, 28: 337-346. 10.1038/emboj.2008.286.PubMed CentralView ArticlePubMedGoogle Scholar
- de Celis JF, Bray S: Feed-back mechanisms affecting Notch activation at the dorsoventral boundary in the Drosophila wing. Development. 1997, 124: 3241-3251.PubMedGoogle Scholar
- Roy M, Pear WS, Aster JC: The multifaceted role of Notch in cancer. Curr Opin Genet Dev. 2007, 17: 52-59. 10.1016/j.gde.2006.12.001.View ArticlePubMedGoogle Scholar
- Hallahan AR, Pritchard JI, Hansen S, Benson M, Stoeck J, Hatton BA, Russell TL, Ellenbogen RG, Bernstein ID, Beachy PA, Olson JM: The SmoA1 mouse model reveals that notch signaling is critical for the growth and survival of sonic hedgehog-induced medulloblastomas. Cancer Res. 2004, 64: 7794-7800. 10.1158/0008-5472.CAN-04-1813.View ArticlePubMedGoogle Scholar
- Curry CL, Reed LL, Golde TE, Miele L, Nickoloff BJ, Foreman KE: Gamma secretase inhibitor blocks Notch activation and induces apoptosis in Kaposi's sarcoma tumor cells. Oncogene. 2005, 24: 6333-6344.PubMedGoogle Scholar
- van Es JH, van Gijn ME, Riccio O, van den Born M, Vooijs M, Begthel H, Cozijnsen M, Robine S, Winton DJ, Radtke F, Clevers H: Notch/gamma-secretase inhibition turns proliferative cells in intestinal crypts and adenomas into goblet cells. Nature. 2005, 435: 959-963. 10.1038/nature03659.View ArticlePubMedGoogle Scholar
- O'Neil J, Calvo J, McKenna K, Krishnamoorthy V, Aster JC, Bassing CH, Alt FW, Kelliher M, Look AT: Activating Notch1 mutations in mouse models of T-ALL. Blood. 2006, 107: 781-785. 10.1182/blood-2005-06-2553.PubMed CentralView ArticlePubMedGoogle Scholar
- Barten DM, Meredith JE, Zaczek R, Houston JG, Albright CF: Gamma-secretase inhibitors for Alzheimer's disease: balancing efficacy and toxicity. Drugs R D. 2006, 7: 87-97. 10.2165/00126839-200607020-00003.View ArticlePubMedGoogle Scholar
- Hasson P, Egoz N, Winkler C, Volohonsky G, Jia S, Dinur T, Volk T, Courey AJ, Paroush Z: EGFR signaling attenuates Groucho-dependent repression to antagonize Notch transcriptional output. Nat Genet. 2005, 37: 101-105.PubMedGoogle Scholar
- Soubeyran P, Kowanetz K, Szymkiewicz I, Langdon WY, Dikic I: Cbl-CIN85-endophilin complex mediates ligand-induced downregulation of EGF receptors. Nature. 2002, 416: 183-187. 10.1038/416183a.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.