- Open Access
Pathobiont release from dysbiotic gut microbiota biofilms in intestinal inflammatory diseases: a role for iron?
© The Author(s). 2019
- Received: 29 August 2018
- Accepted: 21 December 2018
- Published: 3 January 2019
Gut microbiota interacting with an intact mucosal surface are key to the maintenance of homeostasis and health. This review discusses the current state of knowledge of the biofilm mode of growth of these microbiota communities, and how in turn their disruptions may cause disease. Beyond alterations of relative microbial abundance and diversity, the aim of the review is to focus on the disruptions of the microbiota biofilm structure and function, the dispersion of commensal bacteria, and the mechanisms whereby these dispersed commensals may become pathobionts. Recent findings have linked iron acquisition to the expression of virulence factors in gut commensals that have become pathobionts. Causal studies are emerging, and mechanisms common to enteropathogen-induced disruptions, as well as those reported for Inflammatory Bowel Disease and colo-rectal cancer are used as examples to illustrate the great translational potential of such research. These new observations shed new light on our attempts to develop new therapies that are able to protect and restore gut microbiota homeostasis in the many disease conditions that have been linked to microbiota dysbiosis.
- Microbiota biofilm
- Post-infectious IBS
- Inflammatory bowel disease
- Crohn’s disease
- Colo-rectal cancer
The gut microbiome is made of the aggregate collection of genomes and genes contained in its commensal viruses, bacteria, fungi, and Eukarya. Together, these microorganisms are known as the gut microbiota, although at times, the term microbiota is used to specifically refer to the bacterial communities within this complex consortium. The most recent estimates suggest that the ratio of gut bacterial cells to human body cells is approximately 1 to 1 . Gut microbiota live in close association with themselves and with the outer layer of the host mucus, as intricate biofilm communities and free-swimming bacteria that disperse from them. The symbiotic relationship between the host and its gut microbiota begins at birth, and is critical to the overall evolutionary fitness and health of the host [2–4]. Both exogenous as well as endogenous environmental factors are able to modify gut microbiota, and these alterations may in turn lead to detrimental health effects. After elaborating on the biofilm mode of growth of gut microbiota, the present review will focus on how disruptions (“dysbiosis”) within these microbial communities may contribute to enteropathogen-induced disorders, to Inflammatory Bowel Diseases, or to colo-rectal cancer. Microbiota dysbiosis has indeed been linked to a variety of diseases in the gastrointestinal tract as well as in other organs, including the joints, the skin, the eyes, the vasculature, the lungs, and even the central nervous system [4–7]. Much needed causal studies on these relationships are found at increasing rates in the scientific literature. Together, these observations indicate that gut commensal microorganisms may become pathobionts. In humans, animals and plants, pathobionts and opportunistic pathogens are known as temporarily benign microbes or commensals that under environmental or host pressure may cause disease . As much as one third of a host’s metabolome circulating in its blood comes from a bacterial origin, which sheds light on the great potential for gut microbial commensals and pathobionts to shape homeostatic functions throughout the body . While the mechanisms remain largely undefined, this review will present current research findings that are starting to uncover some of these processes.
The well accepted plasticity of the microbiota begs the question of whether the identities of present-day human gut microbial communities originate through inheritance or from environmental pressures. Ground-breaking comparative studies in wild apes, and in humans from Africa or from the Northern hemisphere clearly demonstrate that some of the major families of the gut microbiota have been evolving from common ancestors for more than 15 million years . Since the different species of apes evolved separately from their ancestors, their gut microbiota also diverged and coevolved in parallel, adapting to various environmental factors including diet, gastrointestinal disorders, and habitat, which may in turn have contributed to the significant phyla shifts observed between human gut microbiota of the industrialized World versus those in low income countries [10, 11]. The plasticity of the gut microbiota is reflected in the significant microbiota dysbiosis observed during a variety of disorders. Hence, environmental factors dominate over genetics in determining gut microbiota, which suggests that similar therapeutic approaches aimed at shaping gut microbiota may be applied across different genetic backgrounds. The explosive growth of data on the assembly and stability of gut microbiota, and on how in turn these may control health and disease is both captivating and daunting. In an attempt to reveal common pathways, this review will focus on how disruptions of microbiota biofilms may lead to post-infectious enteritis disorders, to Inflammatory Bowel Diseases (IBD), or to colo-rectal cancer. It has become evident that these emerging views have the potential to revolutionize the development of future therapeutics.
Gut microbiota biofilms
Microbial Biofilms: Aggregates of microbial communities (best characterized for bacteria) that adhere to a surface and to each other, embedded within an extra-cellular matrix made of polysaccharides, proteins, and extracellular DNA. This represents the most common mode of growth of bacteria in nature.
Planktonic bacteria: Bacteria living in single form, swimming or floating in their environment
Pathobionts: Temporarily benign microbes, or commensals, that under environmental or host pressure may cause disease.
Gut microbiome: The aggregate collection of genomes and genes found in the gut microbiota.
Gut microbiota: The polymicrobial communities of viruses, bacteria, Archea, and Eukarya living as commensals in the gut.
Dysbiosis: Structural and /or functional imbalance of the gut microbiota.
Enteropathogen-induced disruptions of microbiota biofilms
In addition to shaping host immunity and gut homeostasis at birth, and to the digestion of undigested nutrients, one of the main functions of the gut microbiota is to protect the host against invading pathogens and against the overgrowth of pathobionts [2, 4]. While these effects are best characterized for bacterial pathogens, they also operate against parasitic and viral pathogens [44–51]. The mechanisms include selective colonization sites, competitive niche exclusion via metabolic interactions and steric hindrance, production of antimicrobials by commensals (eg. bacteriocin), control of pathogen replication, modulation of the mucus barrier, as well as the induction and/or inhibition of specific host immune responses [26, 28, 36, 44, 46, 49–51]. Conversely, much less is known of how enteropathogens are able to affect commensal microbiota. Based on the observations that enteric infections are often followed by post-infectious bouts of Irritable Bowel syndrome, causing flares in patients with IBD, and leading to a variety extra-intestinal complications, recent studies have investigated whether a common pathway whereby these effects may arise is through pathogen-induced disruptions of the gut microbiota, which in turn may drive pathology even when the instigating micro-organism has been cleared [5, 16, 27, 52, 53].
Populations in industrialized countries, with their characteristic high protein and high fat diets, harbour different microbiota than those living in rural areas of developing countries, where a polysaccharide-rich diet is the norm [54, 55]. In rural areas of low income countries, there is an increased representation of mostly gram-negative Bacteroidetes, which can hydrolyze undigestable xylose fibers, whereas gram-positive Firmicutes are the predominant bacterial phylum in high income countries [54, 55]. Both phyla account for more than 95% of the bacteria present in the human gut [4, 5, 7, 11]. The relative sensitivity of these microbiota constituents to enteropathogens remains poorly understood. Recent research from our laboratory and others have now clearly demonstrated that exposure to acute enteropathogens represents yet another important environmental factor able to shape the gut microbiota. The pathogens found to modify human and animal microbiota include bacteria, parasites (Protozoa and Helminths), and viruses (the potential role of Archea is not discussed in this review) [18, 45–51, 56–61]. The mechanisms implicate the release of pathogenic products that may affect both microbiota and host components, modifications of the mucus barrier, redistribution of epithelial Toll-Like Receptors, as well as modulation of host immune responses as least in part by promoting regulatory Tcells that suppress protective responses to inflammatory stimuli [18, 36, 50, 57, 59, 62]. Enteropathogen-modified microbiota directly affect host immunity, and indeed these dysbiotic microbiota are able to cause or exacerbate gut inflammation [18, 19, 36, 45, 59, 62]. Intriguingly, even remote infections, such as respiratory infections with influenza virus, are able to cause gut microbiota dysbiosis . Recent reports also started to shed light on how enteropathogens may modify gut microbiota biofilm phenotype and function . The findings indicate that the Protozoan parasite G. duodenalis directly alters relative bacterial abundance and beta diversity of human microbiota biofilms, most strikingly by increasing the representation of Clostridiales bacteria (belonging to the Firmicutes phylm) . The parasite also disrupts the biofilm exopolysaccharide and promotes the release of pathobionts; these in turn are able to translocate through human epithelia, as well as in germ-free mice, where they induce the production of pro-inflammatory mediators like CXCL-8 and IL-1 . These microbiota alterations do not occur upon exposure to the commensal bacterium Escherichia coli . In contrast, the enteropathogen Campylobacter jejuni is able to modify gut microbiota , and to promote the expression of latent virulent genes in non-invasive E. coli, including fimbrial genes (fimA, sfmF), flagellar genes (fliD), and genes regulating Hemolysin E (hlyE); these effects are associated with disruptions of TLR4 gene expression, and promote the release of pro-inflammatory CXCL-8 in human intestinal epithelial cells . Studies also found that exposure to C. jejuni promotes E. coli adherence to, and subsequent translocation through, intestinal epithelial cells [19, 62]. Translocation is facilitated by hijacking the host lipid raft pathway as well as via epithelial M-cells [62–64]. Other studies demonstrated that infection with C. jejuni indeed exacerbates post-infectious murine colitis upon a mild Dextran Sulfate Sodium (DSS) challenge . Consistent with an enteropathogen-induced dispersion of pathobionts, the transient infection promoted the translocation of commensal bacteria to the spleen and liver, depolarized epithelial TLR9, and worsened post-infectious DSS colitis . These observations shed light on how infections with C. jejuni, and other enteropathogens, including parasites and viruses, may exacerbate inflammation in patients with IBD, lead to post-infectious Irritable Bowel Syndrome, and perhaps contribute to extra-intestinal complications, such as haemolytic-uremic syndrome, endocarditis, and a variety of others [52, 66–71]. Recent studies found that human microbiota rendered dysbiotic by exposure to enteropathogens, of when obtained from patients with IBD, in association with their elevated content of activated virulence genes, cause lethal toxicity in the nematode Caenorhabditis elegans [26, 72]. In situ examination of biopsies from patients with IBD revealed the increased uptake of non-invasive, commensal, E. coli via the follicle-associated epithelial M cells , the same phenomenon found to be facilitated by C. jejuni . In IBD, invading commensal E. coli were shown to co-localize with dendritic cells, which correlated with increased levels of the pro-inflammatory cytokine TNF-α . Finally, noteworthy to this discussion, enteric murine norovirus can add to the function of commensal bacteria and act as a beneficial commensal, in a type I interferon signaling-dependent fashion . While the mechanisms require further investigation, these findings indicate that eukaryotic viruses of the microbiota are capable to support intestinal homeostasis, as do bacterial commensals. This effect goes well beyond the known effects of bacteriophages on commensal microbiota. Viral participation in the microbiota biofilm mode of growth remains obscure. Finally, in addition to direct effects on microbiota through secreted products, enteropathogens may also modulate colonization by infectious or commensal bacteria in the gut by inducing the production of anti-microbial peptides from the host epithelium, and/or by modulating the host inflammatory response; in turn, these effects may attenuate or exacerbate disease symptoms during enteric infection [74–79]. These observations further underscore the significance of microbial-microbial interactions to gut homeostasis.
In summary, exposure to enteropathogens shapes the gut microbiota, throughout life. Importantly in the context of this review, beyond modifying relative bacterial abundance and diversity and modulating host innate immunity, enteropathogens directly modify the phenotype of the gut microbiota biofilm, induce its adherence to the epithelium by allowing it to bypass the mucus barrier, activate latent virulence genes in commensal bacteria, and promote the release of pathobionts which are able to induce and exacerbate intestinal inflammation, in the absence of the instigating pathogen (Figs. 1, 2 and 3). These may contribute to the common occurrence of intestinal and extra-intestinal complications following enteric infections.
Gut microbiota biofilm disruptions in inflammatory bowel diseases and Colo-rectal cancer
Gut microbiota dysbiosis plays a key role in the pathogenesis of Inflammatory Bowel Diseases as well as in the development of colo-rectal cancer and patients’ responses to cancer immunotherapy, but the mechanisms remain incompletely understood. Mice with experimental colitis as well as human patients with colo-rectal cancer contain more bacteria with carcinogenic capabilities. These include colibactin-producing pathobiont strains derived from commensal E. coli as well as other pathobionts, as discussed below . Colibactin acts as a genotoxin that establishes DNA interstrand cross-links in epithelial cells . These studies and others have established a link between inflammatory diseases of the gut and the development of colo-rectal cancer via disruptions of the gut microbiota, adding to the commonly accepted role of inflammatory mediators as carcinogenic DNA damage inducers in gut epithelial cells [82–84]. Alterations in bacterial abundance and diversity in IBD and colo-rectal cancer have been well characterized, and are reviewed elsewhere [4, 5, 82, 85, 86]. In an attempt to identify new therapeutic targets based on shared mechanisms, this discussion will focus instead on the recently proposed role of phenotypic and functional microbiota biofilm alterations in disease pathogenesis [18, 26, 27, 33, 40, 87].
Mucin depletion and microbiota adherence
Degradation of mucosal extracellular matrix components by bacterial-derived metalloproteinases has been suggested to contribute to the pathogenesis of IBD . Whether this bacterial proteolytic activity is also able to alter the exopolysaccharide coat of microbiota biofilms is unclear. We recently demonstrated that the disruptions of microbiota biofilm exopolysaccharides induced by Giardia were triggered at least in part by the pathogen’s cathepsin proteases . These same microbial proteases also exhibit mucinase activity . Mucin depletion is a well-established characteristic of colitis , and represents a preneoplastic lesion in colo-rectal cancer . In patients with IBD, as well as in experimental models of colitis (Fig. 2), gut microbiota biofilm fragments have been observed adhering tightly to the epithelial surface, obviously having bypassed the mucus barrier [33, 35]. Similarly, adherent microbiota biofilm fragments stick to the epithelial surface in polyposis and colorectal cancer . In all instances, this modified biofilm phenotype has been linked to disease development.
Healthy microbiota and pathobionts in IBD and Colo-rectal cancer
Patients with IBD have been found to have twice the concentration of mucosal Bacteroides fragilis biofilm compared with controls; this abnormality can in turn be corrected by antimicrobial therapy . Other pathobionts implicated in the etiology of IBD include E. coli (eg. Adhering Invasive E. coli; AIEC), and Enterococcus faecalis [91–94]. All of these bacterial strains can produce extracellular proteases, lending further support to the hypothesis that these pathobionts may contribute to the pathogenesis of IBD via a proteolytic disruption of gut microbiota biofilms, in addition to their known detrimental effects on host tissue . Indeed some E. coli associated with IBD contain serine protease autotransporter proteins , enterotoxigenic B. fragilis makes a zinc-containing metalloprotease enterotoxin found in IBD , and E. faecalis can produce two extracellular proteases, gelatinase and serine protease, that can induce colitis [95, 96]. The Crohn’s disease pathobiont AIEC, when given to mice as a resident microorganism, exacerbates post-infectious disease upon acute gastroenteritis caused by Salmonella typhymurium or Citrobacter rodentium . Recent findings have demonstrated that microbiota biofilms from patients with Crohn’s disease or with ulcerative colitis are disrupted, and disperse pathobionts that have the ability to invade intestinal epithelia and trigger inflammation . Pathobionts that have been causally implicated in the development of colo-rectal cancer also include Bacteroides fragilis, E. coli, as well as others such as Fusobacterium nucleatum, further linking the inflammatory processes seen in IBD with potential carcinogenesis possibly through strikingly similar microbiota-dependent pathways [80, 83, 98–101].
Healthy gut microbiota also play a key role in anti-cancer immune defense. Recent studies reported that the use of broad-spectrum antibiotics lead to unfavourable clinical outcomes in patients with various types of cancer including colo-rectal cancer . Gut bacteria such as Akkermansia muciniphila, Bacteroides vulgatus, Bifidobacterium spp. and Faecalibacterium spp. (bacterial commensals also linked to protection against Inflammatory Bowel Disease ) have been found to play a key immuno-potentiating role in the cancer-immune dialogue, and contribute to anticancer immunity . Indeed, cause-to-effect relationships between antibiotic-induced microbiota disruptions and failure of immunotherapy have been established in various cancer models treated with programmed cell death protein 1 and anti-CDLA-4 antibodies [102, 105, 106].
Microbiota biofilms in IBD and Colo-rectal cancer
Recent reports suggest that “biofilm formation” is linked to IBD, and may confer a pro-carcinogenic state [35, 40, 41]. Based on the discussion offered above, we propose that it is not the biofilm phenotype of the microbiota per se that may drive disease -the microbiota biofilm mode of growth is homeostatic-, but rather, it is its adhesion to the epithelial surface, beyond the mucus barrier, and its release of pathobionts that should be targeted for therapy. This distinction of course has significant bearing on designing avenues for future therapeutic developments.
The role of iron and metabolomes of pathobionts released from microbiota biofilms
Pathogens have evolved elegant strategies to escape from commensal-induced resistance and host immunity, strategies which in turn confer the microorganisms with effective virulence factors. Production and usage of local luminal metabolites represent key regulators of pathogen-commensal-pathobiont interactions, and are critical for niche selection and for controlling infection and disease [7, 49, 112–115]. As microbiota dysbiosis is associated with changes in the levels of microbial and host metabolites, these in turn offer promise in our quest to discover novel biomarkers and therapeutic targets for microbiota dysbiosis-induced disorders.
The role of iron
Several species of Proteobacteria (Enterobacteriaceae; eg. E. coli, Salmonella, Vibrio) and Firmicutes (Bacillus) need to properly synthesize or incorporate metabolites, including those related to the iron-related purine and pyrimidine metabolism, from their environment to efficiently colonize and persist in the intestine, or to proliferate in the human bloodstream [116–118]. One environmental element that plays a central role in niche selection and virulence is iron. In view of recent findings on the role of iron in pathobiont release from microbiota biofilms in patients with IBD  this section will focus on the role of iron in these processes. Recent findings indicate that genes encoding for propanediol utilization (pdu operon) and iron acquisition (yersiniabactin, chu operon) are overexpressed in AIEC . Furthermore, production of cellulose by AIEC contributes to an iron-dependent promotion of bacterial aggregation, which suggests that iron may have direct and indirect effects on biofilm formation for some species . In vitro , in animal models , and in humans , Proteobacteria can thrive at the expense of other gut bacteria in an iron-rich environment. Thus, iron-acquisition represents a critical factor of bacterial virulence. Furthermore, pathogenic bacteria (including those from the Enterobacteriaceae family) are known to exhibit elevated iron uptake capacity , but data on human mucosal microbiota biofilms were lacking. Recent findings have now established that iron uptake is a key mechanism in conferring virulence to pathobionts dispersed by microbiota biofilms in patients with IBD . Anemia is one of the most common extra-intestinal complication of IBD . Intriguingly, dietary iron supplementation leads to disease exacerbation and a higher risk of infection, perhaps through alterations of commensal microbiota [126–128]. Whether intestinal bleeding associated with IBD could elevate iron concentrations in the intestinal lumen to favour populations of iron-acquiring pathobionts requires further research.
Host lipocalin 2 (Lcn2; also known as siderocalin) impairs iron acquisition by successfully competing with the iron-enterobactin uptake system in bacteria [129, 130]. This protects the host against iron-dependent bacterial pathogenesis and inflammation, including in IBD [131–133]. Lcn2, which is critical for intestinal homeostasis, is increased in the inflamed tissues of patients with IBD for unknown reasons, and is used as a biomarker of inflammation in the gut . Conversely, lower Lcn2 levels in some patients with CD may reflect impaired Th17 immunity in association with the carriage of IBD-risk-increasing IL23R variants, via unclear mechanisms . Knowing that iron can generate powerful radicals and that iron chelators are strong antioxidants  makes the clinical potential of drugs that may chelate iron in IBD even more intriguing. Whether elevated Lcn2 levels in patients with IBD is triggered by the dispersion of pathobionts with high intracellular iron has yet to be established. The significant therapeutic potential of this avenue was recently highlighted in studies that demonstrated that a new drug with potent iron-chelating properties (ATB 429; Antibe Therapeutics, Toronto, Canada) was indeed able to suppress the pathogenic effects of pathobionts that were dispersed by dysbiotic microbiota biofilms from IBD patients .
The microbiota metabolome
A number of studies have established the importance of the microbiota metabolome in health and disease. This rapidly expanding microbiota metabolome linked to human disease includes short chain fatty acids, amines, polysaccharides, primary and secondary bile acids, and various xenobiotic metabolites . Particular attention has been given to bacterial short chain fatty acids, including butyrate, propionate, and acetate. These have been implicated in the modulation of inflammation, in the gut and beyond, and have been linked to the pathogenesis of IBD and cancer [136–140]. Butyrate, which is produced by metabolism of dietary fiber or unabsorbed carbohydrates in the colon, particularly by commensal bacteria belonging to the genus Clostridia, Faecalibacterium, and Roseburia, has been used as a prime example of how short chain fatty acids from the microbiota may regulate key physiological functions in the intestine and other organs [136–138, 140–142]. Mechanisms include the modulation of Wnt signaling, STATS-6-dependent M2 macrophage polarization, induction of the Foxp3 gene to induce Treg cells, epigenetic regulation of gene expression via miRNA, and enhancement of mitochondrial function [137, 141, 143–147]. Moreover, bacterial short chain fatty acids, including butyrate, and amino acids, may inhibit the growth of a variety of gut bacteria . However, recent studies have demonstrated that early intervention with oral sodium-butyrate in neonatal piglets modulates inflammatory cytokines in the ileum, with little impact on intestinal microbiota composition , further highlighting the therapeutic potential of microbiota short chain fatty acids. This potential is being looked at not only for IBD and cancer, but also in a broad range of other diseases in which microbiota dysbiosis has been implicated, including autism spectrum disorders, obesity, kidney disease, and cardio-vascular diseases [140, 147, 149, 150].
In an attempt to assess microbiota metabolomics beyond the microbial secretome in fecal samples, recent analyses of microbiota biofilms grown ex vivo from biopsies of patients with IBD measured and compared the uptake (ie values lower than those of media alone) and release (ie values higher than those of media alone) of microbiota metabolites . The findings indicate that iron chelating compounds increase urate release and reduce guanosine and hypoxanthine uptake in IBD biofilms, in association with the ability of these compounds to block the invasiveness and pro-inflammatoyr phenotype of the dysbiotic microbiota. These may carry physiological significance as elevated serum levels of urate have been associated with chronic inflammatory and metabolic diseases (hypertension atherosclerosis, diabetes), possibly through urate’s pro- and anti-oxidative properties and its interactions with iron . Hypoxanthine, a purine derivative, is used for DNA synthesis as a nutrient by various bacteria, including E. coli and Enterobacteria cloacae [152, 153]. Whether elevated uptake of hypoxanthine by dysbiotic microbiota may contribute to the proliferation of pathogenic Enterobacteriaceae in IBD and in other diseases is unknown.
More research is needed to establish cause-to-effect relationships between microbiota phenotype/function, the microbiota exometabolome, and disease.
A plethora of conditions have been linked to disruption of the gut microbiome. Mechanisms remain incompletely understood, but hold the promise to reveal a path towards new disease markers and therapies. The gut microbiota live in a biofilm mode of growth. The processes promoting this biofilm phenotype in the gut have only begun to emerge. Surface adhesins such as Serine-Rich Repeat Proteins are used by commensal bacteria to form biofilms and for niche selection along the gut. Bacteria from the gut microbiota are enriched with genes linked to biofilm formation, encoding for type IV pili, motility, flagella, intracellular trafficking, adhesins, and biofilm-associated proteins. Biochemical communications between bacteria within the biofilm as well as between planktonic cells, a process known as quorum sensing, modulates the social behaviour of biofilm bacteria via signaling molecules such as cyclic di-GMP, which coordinates some of the steps in the transition between the planktonic and biofilm modes of growth. This review highlights recently discovered pathogenic mechanisms that appear to be shared when induced by enteropathogens, Inflammatory Bowel Disease, and colo-rectal cancer. Beyond the classical disruptions of microbial abundance and diversity, these shared processes involve the disturbance of the gut microbiota biofilm phenotyoe and function, and the dispersion of planktonic bacteria from these commensal communities. These dispersed microorganisms may become pathobionts, and have been causally implicated in disease development, at least in part by invading the mucus barrier, adhering the intestinal epithelium, translocating through enterocytes, and activating various pro-inflammatory pathways, including a recently uncovered mechanism that promotes regulatory T cells which suppress protective responses to inflammatory stimuli. In IBD and colo-rectal cancer, the best characterized bacterial commensals escaping from a disturbed microbiota biofilm and turned pathobionts include Adhering Invasive E. coli (AIEC), Bacteroides fragilis, Enterococcus faecalis, and Fusobacterium nucleatum. Iron-acquisition is a critical factor for bacterial pathogenicity, and recent discoveries have linked this process to the expression of virulence factors in gut commensals that have become pathobionts. More research in the metagenomic, metatranscriptomic, and metabolomic (both for the uptake and release of metabolites) profiles of pathobionts dispersed from dysbiotic gut microbiota biofilms will pave the way towards the developments of new therapies that may restore homeostasis. In view of the numerous clinical conditions that result from microbiota dysbiosis, this field of microbiome research carries enormous translational potential.
Some of the research findings discussed in this review were funded by grants from the Natural Scences and Engineering Research Council of Canada (Discovery grant and NSERC-CREATE grant), the Canadian Institutes of Health Research, Crohn’s and Colitis Canada, the Alberta Ingenuity Fund, the University of Calgary (Eyes High postdoctoral initiative, and the Colon Cancer Screening unit), and AgreenSkillsPlus (PCOFUND-GA-2013-609398).
Some of the research findings discussed in this review were funded by grants from the Natural Scences and Engineering Research Council of Canada (Discovery grant and NSERC-CREATE grant), the Canadian Institutes of Health Research, Crohn’s and Colitis Canada, the Alberta Ingenuity Fund, the University of Calgary (Eyes High postdoctoral initiative, and the Colon Cancer Screening unit), and AgreenSkillsPlus (PCOFUND-GA-2013-609398).
Availability of data and materials
AGB wrote the article. JPM, TA, JF, and JLW provided editorial input for final publication. JPM and TA helped prepare the figures. All authors read and approved the final manuscript.
Ethics approval and consent to participate
This sreview article did not require experimentation.
Consent for publication
All authors have read and approved this article for publication.
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Sender R, Fuchs S, Milo R. Revised estimates for the number of human and bacteria cells in the body. PLoS Boil. 2016;14(18):e1002533.View ArticleGoogle Scholar
- Gordon JI. Honor thy gut symbionts redux. Science. 2012;336:1251–3.PubMedView ArticlePubMed CentralGoogle Scholar
- Sprockett D, Fukami T, Relman DA. Role of priority effects in the early-life assembly of the gut microbiota. Nature Rev Gastroenterol Hepatol. 2018;15:197–205.View ArticleGoogle Scholar
- Backhed F, Fraser CM, Ringel Y, Sanders ME, Sartor RB, Sherman PM, Versalovic J, Yoiung V, Finlay BB. Defining a healthy human gut microbiome: current concepts, future directions, and clinical applications. Cell Host Micr. 2012;12:611–22.View ArticleGoogle Scholar
- Sartor RB, Wu GD. Roles for intestinal bacteria, viruses, and fungi in pathogenesis of inflammatory bowel diseases and therapeutic approaches. Gastroenterology. 2017;152:327–9.PubMedView ArticlePubMed CentralGoogle Scholar
- Sherwin E, Dinan TG, Cryan JF. Recent developments in understanding the role of the gut microbiota in brain health and disease. Ann N Y Acad Sci. 2018;1420:5–25.PubMedView ArticlePubMed CentralGoogle Scholar
- Sekirov I, Russell SL, Antunes CM, Finlay BB. Gut microbiota in health and disease. Physiol Rev. 2010;90:859–904.PubMedView ArticlePubMed CentralGoogle Scholar
- Pitlik SD, Koren O. How holobionts get sick-toward a unifying scheme of disease. Microbiome. 2017;5(64):1–4.Google Scholar
- Nicholson JK, Holmes E, Kinross J, Burcellin R, Gibson G, Jia W, Pettersson S. Host-gut microbiota metabolic interactions. Science. 2012;336(6086):1262–7.PubMedView ArticlePubMed CentralGoogle Scholar
- Moeller AH, Caro-Quintero A, Mjungu D, Georgiev AV, Lonsdorf EV, Muller MN, Pusey AE, Peeters M, Hahn BH, Ochman H. Cospeciation of gut microbiota with hominids. Science 2016. 2018;352(6297):380–2.Google Scholar
- Davenport ER, Sanders JG, Song SJ, Amato KR, Clark AG, Knight R. The human microbiome in evolution. BMC Biol. 2017;15:127–39.PubMedPubMed CentralView ArticleGoogle Scholar
- Round JL, Mazmanian SK. The gut microbiota shapes intestinal immune responses during health and disease. Nat Rev Immunol. 2009;9:313–23.PubMedPubMed CentralView ArticleGoogle Scholar
- Carding S, Verbeke K, Vipond DT, Corfe BM, Owen LJ. Dysbiosis of the gut microbiota in disease. Microbial Ecol Health Dis. 2015;26:26191.Google Scholar
- Costerton JW, Lewandowski Z, Caldwell DE, Korber DR, Lappin-Scott HM. Microbial biofilms. Annu Rev Microbiol. 1995;49:711–45.PubMedView ArticlePubMed CentralGoogle Scholar
- Dunne WM. Bacterial adhesion: seen any good biofilms lately? Clin Microbiol Rev. 2002;15(2):155–66.PubMedPubMed CentralView ArticleGoogle Scholar
- Buret AG. Good bugs, bad bugs in the gut: the role of microbiota dysbiosis in chronic gastrointestinal consequences of infection. Am J Gastroenterol (Suppl). 2016;3:23–31.Google Scholar
- Engel P, Martinson VG, Moran NA. Functional diversity within the simple gut microbiota of the honey bee. PNAS. 2012;109:11002–7.PubMedView ArticlePubMed CentralGoogle Scholar
- Beatty JK, Akierman SV, Motta JP, et al. Giardia duodenalis-induced dysbiosis of human intestinal microbiota biofilms. Int J Parasitol. 2017;47(6):311–26.PubMedView ArticlePubMed CentralGoogle Scholar
- Reti K, Tymensen LD, Davis SP, Amrein MW, Buret AG. Campylobacter jejuni increases flagellar expression and adhesion of non-invasive Escherichia coli: effects on enterocytic TLR-4 and CXCL-8 expression. Infect Immun. 2015;83(12):4571–81.PubMedPubMed CentralView ArticleGoogle Scholar
- Motta JP, Flannigan K, Agbor T, Beatty JK, Blackler RW, Workentine ML, Da Silva GJ, Wang R, Buret AG, Wallace JL. Hydrogen sulfide protects from colitis and restores intestinal microbiota biofilm and mucus production. Infl Bowel Dis. 2015;21(5):1006–17.View ArticleGoogle Scholar
- Clemente JC, Ursell LK, Parfrey LW, Knight R. The impact of the gut microbiota on human health: an integrative review. Cell. 2012;148:1258–70.PubMedPubMed CentralView ArticleGoogle Scholar
- Hopkins MJ, Sharp R, MacFarlane GT. Variation in human intestinal microbiota with age. Dig. Liv. Dis. 2002;34(Suppl):S12–8.Google Scholar
- Von Rosenvinge EC, O’May GA, MacFarlane S, MacFarlane GT, Shirtliff ME. Microbial biofilms and gastrointestinal disease. Pathog Dis. 2013;67:25–38.View ArticleGoogle Scholar
- DeVos WM. Microbial biofilms and the human intestinal microbiome. Npj Biofilms Microbiomes. 2015;1:15005.View ArticleGoogle Scholar
- Probert HM, Gibson GR. Bacterial biofilms in the human gastrointestinal tract. Curr Issues Intest Microbiol. 2002;3:23–7.PubMedPubMed CentralGoogle Scholar
- Motta JP, Allain T, Green-Harrison LE, Groves RA, Feener TD, Ramay H, Beck PL, Lewis IA, Wallace JL, Buret AG. Iron sequestration in microbiota biofilms as a novel strategy for treating inflammatory bowel disease. Inflamm Bowel Dis. 2018;24(7):1493–502.PubMedView ArticlePubMed CentralGoogle Scholar
- Buret AG. Enteropathogen-induced microbiota biofilm disruptions and post-infectious intestinal inflammatory disorders. Curr Trop Med Rep. 2016. https://doi.org/10.1007/s40475-016-0079-x.
- Xu M, Pokrovskii M, Ding Y, Yi R, Au C, Harrison OJ, Galan C, Belkaid Y, Bonneau R, Littman DR. C-MAF -dependent regulatory T cells mediate immunological tolerance to a gut pathobiont. Nature. 2018;554(7692):373–7.PubMedPubMed CentralView ArticleGoogle Scholar
- Gomes-Neto JC, Kittana H, Mantz S, Segura Munoz RR, Schmaltz RJ, Bindels LB, Clarke J, Hostetter JM, Benson AK, Wlater J, Ramer-Tait AE. A gut pathobiont synergizes with the microbiota to instigate inflammatory disease marked by immunoreactivity against other symbionts but not itself. Sci Rep. 2017;7:17707.PubMedPubMed CentralView ArticleGoogle Scholar
- Macfarlane S, Woodmansey EJ, Macfarlane GT. Colonization of mucin by human intestinal bacteria and establishment of biofilm communities in a two-stage continuous culture system. Appl Environ Microbiol. 2005;71:7483–92.PubMedPubMed CentralView ArticleGoogle Scholar
- Eckburg PB, Bik EM, Bernstein CN, Purdom E, Dethlefsen L, Sargent M, Gill SR, Nelson KE, Relman DA. Diversity of the human intestinal microbial flora. Science. 2005;308:1635–8.PubMedPubMed CentralView ArticleGoogle Scholar
- Jones RB, Zhu X, Moan E, Murff HJ, Ness RM, Seidner DL, Sun S, Yu C, Dai Q, Fodor AA, Azcarte-Peril MA, Shrubsole J. Inter-niche and inter-individual variation in gut microbial community assessment using stool, rectal swab, and mucosal samples. Sci Rep. 2018;8:4139.PubMedPubMed CentralView ArticleGoogle Scholar
- Swidsinsky A, Weber J, Loening-Bauke V, Hale LP, Lochs H. Spatial organization and composition of the mucosal flora in patients with IBD. J Clin Microbiol. 2005;43:33803389.Google Scholar
- Lavelle A, Lennon G, O’Sullivan O, Docherty N, Balfe A, Maguire A, Mulcahy HE, Doherty G, O’Donoghue D, Hyland J, Ross RP, Coffey JC, Sheahan K, Cotter PD, Shanahan F, Winter DC, O’Connell PR. Spatial variation of the colonic microbiota in patients with ulcerative colitis and control volunteers. Gut. 2015;64:1553–61.PubMedPubMed CentralView ArticleGoogle Scholar
- Srivastava A, Gupta J, Kumar S, Kumar A. Gut biofilm forming bacteria in IBD. Microb Pathog. 2017;112:5–14.PubMedView ArticleGoogle Scholar
- Amat CB, Motta JP, Fekete E, Moreau F, Chadee K, Buret AG. Cysteine protease-dependent mucus disruptions and differential mucin gene expression in Giardia duodenalis infection. Am J Pathol. 2017;187(11):2486–98.PubMedView ArticleGoogle Scholar
- Chen Y, Peng Y. Fu X. microbial biofilms, colorectal inflammation, and cancer. Austin. J Gastroenterol. 2016;3(1):1059.Google Scholar
- Li S, Konstantinov SR, Smits R, Peppelenbosch MP. Bacterial biofilms in colorectal cancer initiation and progression. Trends Mol Med. 2017;23(1):18–30.PubMedView ArticleGoogle Scholar
- Lane ER, Zisman TL, Suskind DL. The microbiota in inflammatory bowel disease: current and therapeutic insights. J Inflamm Res. 2017;10:63–73.PubMedPubMed CentralView ArticleGoogle Scholar
- Dejea CM, Fahti P, Craig JM, Boleij A, Taddese R, Geis AL, WU X, DeStefano Shields CE, Hechenbleikner EM, Huso DL, Anders RA, Giardello FM, Wick EC, Wang H, Wu S, Pardoll DM, Housseau F, Sears CL. Patients with familial adenomatous polyposis harbor colonic biofilms containing tumorigenic bacteria. Science. 2018;359:592–7.PubMedPubMed CentralView ArticleGoogle Scholar
- Dejea C, Sears CL. Do biofilms confer a pro-carcinogenic state? Gut Microbes. 2016;7(1):54–7.PubMedPubMed CentralView ArticleGoogle Scholar
- Ni J, Wu GD, Albenberg L, Tomov VT. Gut microbitoa and IBD: causation or correlation? Nat Rev Gastroenterol Hepatol. 2017. https://doi.org/10.1038/nrgastro.2017.88.
- Sun M, Wei W, Liu Z, Cong Y. Microbiota metabolite short chain fatty acids, GPCR, and inflammatory bowel disease. J Gastroenterol. 2017;52:1–8.PubMedView ArticleGoogle Scholar
- Ubeda C, Djukovic A, Isaac S. Roles of the intestinal microbiota in pathogen protection. Clin Transl Immunol. 2017;6(2):e128.View ArticleGoogle Scholar
- Garcia-Gutierrez E, Mayer MJ, Cotter PD, Narbad A. Gut microbiota as a source of novel antimicrobials. Gut Microbes. 2018:1–21.Google Scholar
- Wilks J, Beilinson H, Golovkina T. Dual role of commensal bacteria in viral infections. Immunol Rev. 2013;255(1). https://doi.org/10.1111/imr.12097.
- Allain T, Amat CB, Motta JP, Manko A, Buret AG. Interactions of Giardia sp with the intestinal barrier: epithelium, mucus, and microbiota. Tissue Barriers. 2017;5(1):e1274354.PubMedPubMed CentralView ArticleGoogle Scholar
- Singer SM, Nash TE. The role of normal flora in Giardia lamblia infections in mice. J Infect Dis. 2000;181(4):1510–2.PubMedView ArticleGoogle Scholar
- Kamada N, Chen GY, Inohara N, Nunez G. Control of pathogens and pathobionts by the gut microbiota. Nat Immunol. 2013;14(7):685–90.PubMedPubMed CentralView ArticleGoogle Scholar
- Rolhion N, Chassaing B. When pathogenic bacteria meet the intestinal microbiota. Philos Trans R Soc Lond Ser B Biol Sci. 2016;37(1707):20150504.View ArticleGoogle Scholar
- Baumler AJ, Sperandio V. Interactions between the microbiota and pathogenic bacteria in the gut. Nature. 2016;535(7610):85–93.PubMedPubMed CentralView ArticleGoogle Scholar
- Halliez MC, Buret AG. Extra-intestinal and long term consequences of Giardia duodenalis infections. World J Gastroenterol. 2013;19(47):8974–85.PubMedPubMed CentralView ArticleGoogle Scholar
- Gupta S. Something in the water. Nature. 2016;533:S114–5.PubMedView ArticlePubMed CentralGoogle Scholar
- De Filippo C, Cavalieri D, Di Paola M, Ramazzotti M, Poullet JB, Massart S, Collini S, Pieraccini G, Lionetti P. Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa. Proc Natl Acad Sci U S A. 2010;107:14691–6.PubMedPubMed CentralView ArticleGoogle Scholar
- Das B, Ghosh TS, Kedia S, Rampal R, Saxena S, Bag S, Mitra R, Dayal M, Mehta O, Surendranath A, Travis SPL, Tripathi P, Nair GB, Ahuja V. Analysis of the gut microbiome of rural and urban healthy Indians living in sea level and high altitude areas. Sci Rep. 2018;8:10104.PubMedPubMed CentralView ArticleGoogle Scholar
- Su C, Su L, Li Y, Chang J, Zhang W, Walker WA, Xavier R, Cherayil BJ, Shi HN. Helminth-induced alterations of the gut microbiota exacerbate bacterial colitis. Muc Immunol. 2018;11(1):144–57.View ArticleGoogle Scholar
- Kembauer E, Ding Y, Cadwell K. An enteric virus can replace the beneficial function of commensal bacteria. Nature. 2014;516(7529):94–8.Google Scholar
- Yildiz S, Mazel-Sanchez B, Kandasamy M, Manicassamy B, Schmolke M. Influenza a virus infection impacts systemic microbiota dynamics and causes quantitative enteric dysbiosis. Microbiome. 2018;6:9.PubMedPubMed CentralView ArticleGoogle Scholar
- Hoffmann C, Hill DA, Minkah N, Kirn T, Troy A, Artis D, Bushman F. Community-wide response of the gut microbiota to enteropathogenic Citrobacter rodentium infection revealed by deep sequencing. Infect Immun. 2009;77(10):4668–78.PubMedPubMed CentralView ArticleGoogle Scholar
- Arguello H, Estelle J, Zaldivar-Lopez S, Jimenez-Marin A, Carvajal A, Lopez-Bascon MA, Cripie F, O’Sullivan O, Cotter PD, Priego-Capote F, Mmorera L, Garrido JJ. Early Salmonella typhimurium infection in pigs disrupts microbiome composition and functionality at the ileum mucosa. Sci Rep. 2018;8:7788.PubMedPubMed CentralView ArticleGoogle Scholar
- Lone AG, Selinger B, Uwiera RRE, Xu Y, Inglis D. Campylobacter jejuni colonization is associated with a dysbiosis in the cecal microbiota of mice in the absence of prominent inflammation. PLoS One. 2013;8(9):e75325.PubMedPubMed CentralView ArticleGoogle Scholar
- Lamb-Rosteski JM, Kalischuk LD, Inglis GD, Buret AG. Epidermal growth factor inhibits Campylobacter jejuni-induced claudin-4 disruption, loss of epithelial barrier function, and Escherichia coli translocation. Infect Immun. 2008;76(8):3390–8.PubMedPubMed CentralView ArticleGoogle Scholar
- Kalischuk LD, Inglis GD, Buret AG. Campylobacter jejuni induces transcellular translocation of commensal bacteria via lipid rafts. Gut Pathog. 2009;1:2.PubMedPubMed CentralView ArticleGoogle Scholar
- Kalischuk LD, Leggett F, Inglis GD. Campylobacter jejuni induces transcytosis of commensal bacteria across the intestinal epithelium through M-cells. Gut Pathog. 2010;2:14.PubMedPubMed CentralView ArticleGoogle Scholar
- O’Hara JR, Feener TD, Fischer CD, Buret AG. Campylobacter jejuni disrupts protective toll-like receptor 9 signaling in colonic epithelial cells and increases the severity of dextran sulfate sodium-induced colitis in mice. Infect Immun. 2012;80(4):1563–71.PubMedPubMed CentralView ArticleGoogle Scholar
- Kalischuk LD, Buret AG. A role for Campylobacter jejuni-induced enteritis in inflammatory bowel disease? Am J Phys. 2010;298(1):G1–9.View ArticleGoogle Scholar
- Gradel KO, Nielsen HL, Shonheyder HC, Ejlertsen T, Kristensen B, Nielsen H. Increased short- and long-term risk of inflammatory bowel disease after salmonella or campylobacter gastroenteritis. Gastroenterology. 2009;137(2):495–501.PubMedView ArticlePubMed CentralGoogle Scholar
- Rodriguez LAG, Ruigomez A, Panes J. Acute gastroenteritis is followed by an increased risk of inflammatory bowel disease. Gastroenterology. 2006;130(6):1588–94.View ArticleGoogle Scholar
- Porter CK, Faix DJ, Shiau D, Espiritu J, Espinosa BJ, Riddle MS. Postinfectious gastrointestinal disorders following norovirus outbreaks. Clin Infect Dis. 2012;55(7):915–22.PubMedView ArticlePubMed CentralGoogle Scholar
- Delans RJ, Biuso JD, Saba SR, Ramirez G. Hemolytic uremic syndrome after campylobacter-induced diarrhea in an adult. Arch Intern Med. 1984;144(5):1074–6.PubMedView ArticlePubMed CentralGoogle Scholar
- Dinant S, Schurink CAM, Deckers JW, Severin JA. Aortic homograft endocarditis caused by Campylobacter jejuni. J Clin Microbiol. 2011;49(11):4016–7.PubMedPubMed CentralView ArticleGoogle Scholar
- Gerbaba TK, Gupta P, Rioux K, Hansen D, Buret AG. Giardia-duodenalis -induced alterations of commensal bacteria kill Caenorhabditis elegans: a new model to study microbial-microbial interactions in the gut. Am J Physiol (Gastro Liv Physiol). 2015;308(6):G550–61.Google Scholar
- Roberts CL, Keita AV, Duncan SH, O’Kennedy N, Soderholm JD, Campbell BJ. Translocation of Crohn’s disease Escherichia coli across M-cells: contrasting effects of soluble plant fibers and emulsifiers. Gut. 2010;59(10):1331–9.PubMedPubMed CentralView ArticleGoogle Scholar
- Manko A, Motta JP, Cotton JA, Feener TD, Oyeyemi A, Vallance BA, Wallace JL, Buret AG. Giardia co-infection promotes the secretion of antimicrobial peptide Beta-defensin 2 and trefoil factor 3 and attenuates attaching and effacing Bacteria-induces intestinal disease. PLoS One. 2017;12(6):e0178647.PubMedPubMed CentralView ArticleGoogle Scholar
- Cotton JA, Amat CB, Buret AG. Disruptions of host immunity and inflammation by Giardia duodenalis: potential consequences for co-infections in the gastro-intestinal tract. Pathogens. 2015;4(4):764–92.PubMedPubMed CentralView ArticleGoogle Scholar
- Cotton JA, Motta JP, Schenk LP, Hirota SA, Beck PL, Buret AG. Giardia duodenalis infection reduces granulocyte infiltration in an in vivo model of bacterial toxin-induced colitis and attenuates inflammation in human intestinal tissue. PLoS One. 2014;9(10):e109087.PubMedPubMed CentralView ArticleGoogle Scholar
- Cotton JA, Bhargava A, Ferraz J, Yates R, Beck PL, Buret AG. Giardia duodenalis cathepsin B proteases degrade intestinal epithelial interleukin-8 and attenuate interleukin-8-induced neutrophil chemotaxis. Infect Immun. 2014;82(7):2772–87.PubMedPubMed CentralView ArticleGoogle Scholar
- McKay DM. Not all parasites are protective. Parasite Immunol. 2015;37:324–32.PubMedView ArticlePubMed CentralGoogle Scholar
- Burgess SL, Gilchrist CA, Tucker CL, Petri WA. Parasitic Protozoa and interactions with host intestinal microbiota. Infect Immun. 2017;85(8):e00101–17.PubMedPubMed CentralView ArticleGoogle Scholar
- Arthur JC, Perez-Chanona E, Muhlbauer M, Tomkovich S, Uronis JM, Fan TJ, Campbell BJ, Abujamel T, Dogan B, Rogers AB, Rhodes JM, Stintzi A, Simpson KW, Hansen JJ, Keku TO, Fodor AA, Jobin C. Intestinal inflammation targets cancer-inducing activity of the microbiota. Science. 2012;338(6103):120–3.PubMedPubMed CentralView ArticleGoogle Scholar
- Bossuet-Greif N, Vignard J, Taieb F, Mirey G, Dubois D, Petit C, Oswald E, Nougayrede J-P. The colibactin genotoxin generates DNA interstrand cross-links in infected cells. mBio. 2018;9:e02393–17.PubMedPubMed CentralView ArticleGoogle Scholar
- Gopalakrishnan V, Helmink BA, Spencer CN, Reuben A, Wargo JA. The influence of the gut microbiome on cancer, immunity, and cancer immunotherapy. Cancer Cell. 2018;33:570–80.PubMedView ArticlePubMed CentralGoogle Scholar
- Jobin C. Human intestinal microbiota and colorectal cancer: moving beyond associative studies. Gastroenterology. 2017;153(6):1475–8.PubMedView ArticlePubMed CentralGoogle Scholar
- Greten FR, Eckmann L, Greten TF, Park GM, Li ZW, Egan LJ, Kagnoff MF, Karin M. IKKβ links inflammation and tumorigenesis in a mouse model of colitis-associated cancer. Cell. 2004;118(3):285–96.PubMedView ArticlePubMed CentralGoogle Scholar
- Goodman B, Gardner H. The microbiome and cancer. J Pathol. 2018;244(5):667–76.PubMedView ArticlePubMed CentralGoogle Scholar
- Nishida A, Inoue R, Inatomi O, Bamba S, Naito Y, Andoh A. Gut microbiota in the pathogenesis of inflammatory bowel disease. Clin J Gastroenterol. 2018;11(1):1–10.PubMedView ArticlePubMed CentralGoogle Scholar
- Drewes JL, White JR, Dejea CM, Fathi P, Iyadorai T, Vadivelu J, Roslani AC, Wick EC, Mongodin EF, Loke MF, Thulasi K, Gan HM, Goh KL, Chong HY, Kumar S, Wanyiri JW, Sears CL. High-resolution bacterial 16S rRNA gene profile meta-analysis and biofilm status reveal common colorectal cancer consortia. Npj Biofilms and Microbiomes. 2017;3:34.PubMedPubMed CentralView ArticleGoogle Scholar
- Pruteanu M, Hyland NP, Clarke DJ. Degradation of the extra-cellular matrix components by bacterial-derived metalloproteinases: implications for inflammatory bowel diseases. Inflamm Bow Dis. 2010;17:1189–200.View ArticleGoogle Scholar
- Dwarakanath AD, Campbell BJ, Tsai HH. Faecal mucinase activity assessed in inflammatory bowel disease using 14C threonine labelled mucin substrate. Gut. 1995;37:58–62.PubMedPubMed CentralView ArticleGoogle Scholar
- Sakai E, Morioka T, Yamada E, Ohkubo H, Higurashi T, Hosono K, Endo H, Takahashi R, Cui C, Shiozawa M, Akaike M, Samura H, Nishimaki T, Nakajima A, Yoshimi N. Identification of preneoplastic lesions as mucin-depleted foci in patients with sporadic colorectal cancer. Cancer Sci. 2012;103(1):114–49.View ArticleGoogle Scholar
- Kotlowsky R, Bernstein CN, Sepehri S. High prevalence of Escherichia coli belonging to the B2+D phylogenetic group in inflammatory bowel disease. Gut. 2007;56:669–75.View ArticleGoogle Scholar
- Prindiville TP, Sheikh RA, Cohen SH. Bacteroides fragilis enterotoxin gene sequences in patients with inflammatory bowel disease. Emerg Infect Dis. 2000;6:171–4.PubMedPubMed CentralView ArticleGoogle Scholar
- Darfeuille-Michaud A, Boudeau J, Bulois P, Neut C, Glasser AL, Barnich N. High prevalence of adherent-invasive Escherichia coli associated with ileal mucosa in Crohn’s disease. Gastroenterology. 2004;127:412–21.PubMedView ArticlePubMed CentralGoogle Scholar
- Butto LF, Schaubeck M, Haller D. Mechanisms of microbe-host interaction in Crohn’s disease: Dysbiosis vs. pathobiont selection. Front Immunol. 2015. https://doi.org/10.3389/fimmu.2015.00555.
- Qin X, Singh KV, Weinstick GM. Effects of enterococcus faecalis fsr genes on production of gelatinase and a serine protease and virulence. Infect Immun. 2000;68:2579–86.PubMedPubMed CentralView ArticleGoogle Scholar
- Balish E, Warner T. Enterococcus faecalis induces inflammatory bowel disease in interleukin-10 knockout mice. Am J Pathol. 2002;160:2253–7.PubMedPubMed CentralView ArticleGoogle Scholar
- Small CL, Xing L, McPhee JB, Law TL, Coombes BK. Acute infectious gastroenteritis potentiates a Crohn’s disease pathobiont to fuel ongoing inflammation in the post-infectious period. PLoS Pathog. 2016;12(10):e1005907.PubMedPubMed CentralView ArticleGoogle Scholar
- Sears CL, Geis AL, Housseau F. Bacteroides fragilis subverts mucosal biology: from symbiont to colon carcinogenesis. J Clin Invest. 2014;124:4166–72.PubMedPubMed CentralView ArticleGoogle Scholar
- Schwabe RF, Jobin C. The microbiome and cancer. Nat Rev. 2013;13:800–12.View ArticleGoogle Scholar
- Wong SH, Zhao L, Zhang X. Gavage of fecal samples from patients with colorectal cancer promotes intestinal carcinogenesis in germ-free and conventional mice. Gastroenterology. 2017;153:1621–33.PubMedView ArticlePubMed CentralGoogle Scholar
- Chen W, Liu F, Ling Z, Tong X, Xiang C. Human intestinal lumen and mucosa-associated microbiota in patients with colorectal cancer. PLoS One. 2012;7(6):e39743.PubMedPubMed CentralView ArticleGoogle Scholar
- Routy B, et al. Gut microbiome influences efficacy of PD-1-based immunotherapy against epithelial tumors. Science. 2018;359:91–7.PubMedView ArticlePubMed CentralGoogle Scholar
- Sokol H, Pigneur B, Watterlot L, Lakhdari O, Bermudez-Humaran LG, et al. Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients. PNAS. 2008;105(43):16731–6.PubMedView ArticlePubMed CentralGoogle Scholar
- Routy B, Gopalakrishnan V, Daillere R, Zitvogel L, Wargo JA, Kroemer G. The gut microbiota influences anticancer immunosurveillance and general health. Nat Rev Clin Oncol. 2018;15:382–96.PubMedView ArticlePubMed CentralGoogle Scholar
- Gopalakrishnan V, Spencer CN, Nezi L, Reuben A, Andrews MC, Karpinets TV, Prieto PA, Vicente D, et al. Gut microbiome modulates response to anti-PD-1 immunotherapy in melanoma patients. Science. 2018;359(6371):97–103.PubMedView ArticlePubMed CentralGoogle Scholar
- Matson V, Fessler J, Bao R, Chgonsuwat T, Zha Y, Alegre ML, Luke J, Gajewski TF. The commensal microbiome is associated with anti -PD-1 efficacy in metastatic melanoma patients. Science. 2018;359(6371):104–8.PubMedView ArticlePubMed CentralGoogle Scholar
- Frese SA, MacKenzie DA, Peterson DA, Schmaltz R, Fangman T, Zhou Y, Zhang C, Benson AK, Cody LA, Mulholland F, Juge N, Walter J. Molecular characterization of host-specific biofilm fomrmation in a vertebrate gut symbiont. PLoS Genet. 2013;9(12):e1004057.PubMedPubMed CentralView ArticleGoogle Scholar
- Sequeira S, Kananaugh D, MacKenzie DA, Suligoj T, Walpole S, Leclaire C, Gunning AP, Latousakis D, Willats WGT, Angulo J, Dong C, Juge N. Structural basis for the role of serne-rich repeat proteins from lactobacillus reuteri in gut microbe-host interactions. PNAS. 2018;115(12):E2706–15.PubMedView ArticlePubMed CentralGoogle Scholar
- Mukherjee S, Moustafa D, Smith CD, Goldberg JB, Bassler BL. The RhIR quorum-sensing receptor controls Pseudomonas aeruginosa pathogenesis and biofilm development independently of its canonical homoserine lactone autoinducer. PLoS Pathog. 2017;13:e1006504.PubMedPubMed CentralView ArticleGoogle Scholar
- Sharma IM, Petchiappan A, Chatterji D. Quorum sensing and biofilm formation in mycobacteria: role of c-di-GMP and methos to study this second messenger. IUBMB Life. 2014;66:823–34.PubMedView ArticlePubMed CentralGoogle Scholar
- Valentini M, Filloux A. Biofilms and cyclic di-GMP signlaing: lessons from Pseudomonas aeruginosa and other bacteria. J Biol Chem. 2016;291:12547–55.PubMedPubMed CentralView ArticleGoogle Scholar
- Zierer J, Jackson MA, KAstenmueller G, Mangino M, Long T, Telenti A, Mohney RP, Small KS, Bell JT, Steves CJ, Valdes AM, Spector TD, Menni C. The fecal metabolome as a functional readout of the gut microbiome. Nat Genet. 2018;50:790–5.PubMedPubMed CentralView ArticleGoogle Scholar
- Martens EC, Neumann M, Desai MS. Interactions of commensal and pathogenic microorganisms with the intestinal mucosal barrier. Nat Rev Microbiol. 2018;16:457–70.View ArticleGoogle Scholar
- Gilbert JA, Blaser MJ, Caporaso JG, Jansson JK, Lynch SV, Knight R. Current understanding of the human microbiome. Nature Med. 2018;24:392–400.PubMedView ArticlePubMed CentralGoogle Scholar
- Nutritional preferences of human gut bacteria reveal their metabolic idiosyncrasies. Nat Microbiol. 2018;3:514–22.View ArticleGoogle Scholar
- Samant S, Lee H, Ghassemi M, et al. Nucleotide biosynthesis is critical for growth of bacteria in human blood. PLoS Pathog. 2008;4:e37.PubMedPubMed CentralView ArticleGoogle Scholar
- Vogel-Scheel J, Alpert C, Engst W, Loh G, Blaut M. Requirement of purine and pyrimidine synthesis for colonization of the mouse intestine by Escherichia coli. Appl Environ Microbiol. 2010;76:5181–7.PubMedPubMed CentralView ArticleGoogle Scholar
- Chiang SL, Mekalanos JJ. Use of signature-tagged transposon mutagenesis to identify Vibrio cholerae genes critical for colonization. Mol Microbiol. 1998;27:797–805.PubMedView ArticlePubMed CentralGoogle Scholar
- Dogan B, Suzuki H, Herlekar D, et al. Inflammation-associated adherent-invasive E. coli are enriched in pathways for use of propanediol and iron and M-cell translocation. Inflamm Bowel Dis. 2014;20(11):1919–32.PubMedView ArticlePubMed CentralGoogle Scholar
- Ellerman M, Huh EY, Carroll IM, et al. Adherent-invasive E.coli porduction of cellulose influences iron-induced bacterial aggregation, phagocytosis, and induction of colitis. Infect Immun. 2015;83(10):4068–80.View ArticleGoogle Scholar
- Kortman GA, Dutilh BE, Maathuis AJ, et al. Microbial metabolism shifts towards an adverse profile with supplementary iron in the tim-2 in vitro model of the human colon. Front Microbiol. 2015;6:1481.PubMedPubMed CentralGoogle Scholar
- Werner T, Wagner SJ, Martinez I, et al. Depletion of luminal iron alters the gut microbiota and prevents Crohn's disease-like ileitis. Gut. 2011;60:325–33.PubMedView ArticlePubMed CentralGoogle Scholar
- Jaeggi T, Kortman GA, Moretti D, Chassard C, Holding P, Dostal A, Boekhorst J, Timmerman HM, Swinkels DW, Tjalsma H, Njenga J, Mwangi A, Kvalsvig J, Lacroix C, Zimmermann MB. Iron fortification adversely affects the gut microbiome, increases pathogen abundance and induces intestinal inflammation in Kenyan infants. Gut. 2015;64:731–42.PubMedView ArticlePubMed CentralGoogle Scholar
- Skaar EP. The battle for iron between bacterial pathogens and their vertebrate hosts. PLoS Pathog. 2010;6:e1000949.PubMedPubMed CentralView ArticleGoogle Scholar
- Stein J, Hartmann F, Dignass AU. Diagnosis and management of iron deficiency anemia in patients with IBD. Nat Rev Gastroenterol Hepatol. 2010;7:599–610.PubMedView ArticlePubMed CentralGoogle Scholar
- Lee T, Clavel K, Smirnov A, et al. Oral versus intravenous iron replacement therapy distinctly alters the gut microbiota and metabolome in patients with IBD. Gut. 2017;66:863–71.PubMedView ArticlePubMed CentralGoogle Scholar
- Uritski R, Barshack I, Bilkis I, et al. Dietary iron affects inflammatory status in a rat model of colitis. J Nutr. 2004;134:2251–5.PubMedView ArticlePubMed CentralGoogle Scholar
- Kortman GA, Raffatellu M, Swinkels DW, et al. Nutritional iron turned inside out: intestinal stress from a gut microbial perspective. FEMS Microbiol Rev. 2014;38:1202–34.PubMedView ArticlePubMed CentralGoogle Scholar
- Fishbach MA, Lin H, Zhou L, et al. The pathogen-associated iroA gene cluster mediates bacterial evasion of lipocalin 2. Proc Natl Acad Sci U S A. 2006;103:16502–7.View ArticleGoogle Scholar
- Goetz DH, Holmes N, Borregaard N, et al. The neutrophil lipocalin NGAL is a bacteriostatic agent that interferes with siderophore-mediated iron acquisition. Mol Cell. 2002;10:1033–43.PubMedView ArticlePubMed CentralGoogle Scholar
- Singh V, Yeoh BS, Chassaing B, et al. Microbiota-inducible innate immune siderophore binding protein lipocalin 2 is critical for intestinal homeostasis. Cell Mol Gastroenterol Hepatol. 2016;2(4):482–488.e6.PubMedPubMed CentralView ArticleGoogle Scholar
- Toyonaga T, Matsuura M, Mori K, et al. Lipocalin 2 prevents intestinal inflammation by enhancing phagocytic bacterial clearance in macrophages. Sci Rep. 2016;6:35014.PubMedPubMed CentralView ArticleGoogle Scholar
- Stallhofer J, Friedrich M, Konrad-Zerna A, et al. Lipocalin 2 is a disease activity marker in inflammatory bowel disease regulated by IL-17A, IL-22, and TNFalpha, and modulated by IL23R genotype status. Inflamma. Bowel Dis. 2015;21(10):2327–40.Google Scholar
- Jomova K, Valko M. Importance of iron chelation in free radical-induced oxidative stress and human disease. Curr Pharm Des. 2011;17:3460–73.PubMedView ArticlePubMed CentralGoogle Scholar
- Brown JM, Hazen SL. Targeting of microbe-derived metabolites to improve human health: the next frontier for drug discovery. J Biol Chem. 2017;292:8560–8.PubMedPubMed CentralView ArticleGoogle Scholar
- Thio CL, Chi PY, Lai AC, Chang YJ. Regulation of type 2 innate lymphoid cell-dependent airway hyperreactivity by butyrate. J Allergy Clin Immunol. 2018:S0091–6749.Google Scholar
- Uchiyama K, Sakiyama T, Hasebe T, Musch MW, Miyoshi H, Nakagawa Y, He TC, Lichtenstein L, Naito Y, Itoh Y, Yoshikawa T, Jabri B, Stappenbeck T, Chang EB. Butyrate and bioactive proteolytic form of Wnt-5a regulate colonic epithelial proliferation and spatial development. Sci Rep. 2016;6:32094.PubMedPubMed CentralView ArticleGoogle Scholar
- Cuff MA, Shirazi-Beechey SP. The importance of butyrate transport to the regulation of gene expression in the colonic epithelium. Biochem Soc Transact. 2004;32:1100–2.View ArticleGoogle Scholar
- Comalda M. The effects of short chain fatty acids on colon epithelial proliferation and survival depend on the cellular phenotype. J Cancer Res Clin Oncol. 2006;132:487–97.View ArticleGoogle Scholar
- Brown JM, Hazen SL. Microbial modulation of cardiovascular disease. Nat Rev Microbiol. 2018;16:171–81.PubMedPubMed CentralView ArticleGoogle Scholar
- Smith PM. The microbial metabolites, short-chain fatty acids, regulate colonic Treg cell homeostasis. Science. 2013;341:569–73.PubMedView ArticleGoogle Scholar
- Koh A, De Vadder F, Kavatcheva-Datchary P, Backhed F. From dietary fiber to host physiology: short-chain fatty acids as key bacterial metabolites. Cell. 2016;165:1332–45.PubMedView ArticlePubMed CentralGoogle Scholar
- Ji J, Shu D, Zheng M, Wang J, Luo C, Wang Y, Guo F, Zou X, Lv X, Li Y, Liu T, Qu H. Microbial metabolite butyrate facilitates M2 macrophage polarization and function. Sci Rep. 2016;6:24838.PubMedPubMed CentralView ArticleGoogle Scholar
- Atarashi K. Treg induction by a rationally selected mixture of clostridia strains from the human microbiota. Nature. 2013;500:232–6.PubMedView ArticlePubMed CentralGoogle Scholar
- Furusawa Y. Commensal microbe-derived butyrate induces the differentiation of colonic regulatory T cells. Nature. 2013;504:446–50.PubMedView ArticlePubMed CentralGoogle Scholar
- Hu S, Dong TS, Delai SR, Wu F, Bissonnette M, Kwon JH, Chang EB. The microbe-derived short chain fatty acid butyrate targets miRNA -dependent p21 gene expression in human colon cancer. PLoS One. 2011;6(1):e16221.PubMedPubMed CentralView ArticleGoogle Scholar
- Rose S, Bennuri SC, Davis JE, Wynne R, Slattery JC, Tippett M, Delhey MS, Kahler SG, MacFabe DF, Frye RE. Butyrate enhances mitochondrial function during oxidative stress in cell lines from boys with autism. Transl Psych. 2018;8:42.View ArticleGoogle Scholar
- Xu J, Chen X, Yu S, Su Y, Zhu W. Effects of early intervention with sodium butyrate on gut microbiota and the expression of inflammatory cytokines in neonatal piglets. PLoS One. 2016;11(9):e0162461.PubMedPubMed CentralView ArticleGoogle Scholar
- Ramezani A. Role of the gut microbiome in uremia: a potential therapeutic target. Am J Kidney Dis. 2016;67:483–98.View ArticleGoogle Scholar
- Canfora EE, Jocken JW, Blaak EE. Short-chain fatty acids in control of body weight and insulin sensitivity. Nat Rev Endocrinol. 2015;11:577–91.PubMedView ArticlePubMed CentralGoogle Scholar
- So A, Thorens B. Uric acid transport and disease. J Clin Invest. 2010;120:1791–9.PubMedPubMed CentralView ArticleGoogle Scholar
- Machida Y, Nakanishi T. Aerobic degradation of purines by Enterobacter cloacae. Ag Biol Chem. 1982;46(8):2135–6.Google Scholar
- Shivashankar K, Subbayya IN, Balarma H. Developmnet of a bacterial screen for novel hypoxanthine-guanine phosphoribosyltransferase substrates. J Mol Microbiol Biotechnol. 2001;3(4):557–62.PubMedGoogle Scholar