3D brain Organoids derived from pluripotent stem cells: promising experimental models for brain development and neurodegenerative disorders
© The Author(s). 2017
Received: 23 June 2017
Accepted: 9 August 2017
Published: 20 August 2017
Three-dimensional (3D) brain organoids derived from human pluripotent stem cells (hPSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), appear to recapitulate the brain’s 3D cytoarchitectural arrangement and provide new opportunities to explore disease pathogenesis in the human brain. Human iPSC (hiPSC) reprogramming methods, combined with 3D brain organoid tools, may allow patient-derived organoids to serve as a preclinical platform to bridge the translational gap between animal models and human clinical trials. Studies using patient-derived brain organoids have already revealed novel insights into molecular and genetic mechanisms of certain complex human neurological disorders such as microcephaly, autism, and Alzheimer’s disease. Furthermore, the combination of hiPSC technology and small-molecule high-throughput screening (HTS) facilitates the development of novel pharmacotherapeutic strategies, while transcriptome sequencing enables the transcriptional profiling of patient-derived brain organoids. Finally, the addition of CRISPR/Cas9 genome editing provides incredible potential for personalized cell replacement therapy with genetically corrected hiPSCs. This review describes the history and current state of 3D brain organoid differentiation strategies, a survey of applications of organoids towards studies of neurodevelopmental and neurodegenerative disorders, and the challenges associated with their use as in vitro models of neurological disorders.
Keywords3D brain organoids Induced pluripotent stem cells Brain development Neocortex Neurodevelopmental disorder Neurodegenerative disorder Microcephaly Drugs Autism Alzheimer’s disease
In recent years, genomic studies of individuals with developmental or neurodegenerative diseases have tremendously advanced our understanding of the genetics of these disorders [1–5]. Many of them, including autism spectrum disorder (ASD), schizophrenia, Alzheimer’s disease (AD), and Parkinson’s disease (PD), are caused by a heterogeneous combination of variant alleles and have therefore proven difficult to recapitulate in animal models, which are better suited for studying single-gene mutations [6–9]. Although the human brain is the ideal model to study human neuropathology, the limited availability of healthy and diseased brain tissue as well as the difficulties with culturing or genetically manipulating the tissue makes this an inopportune model with which to easily elucidate molecular mechanisms underlying these disorders. Recent progress in iPSC technology has provided a remarkable alternative for the study of human brain diseases through the scalable, manipulatable production of human neural cells derived directly from patients with diverse neurological diseases [10–15]. However, the potential of hiPSCs to recapitulate complex human brain disorders remains incompletely exploited. In this review, we summarize the current state of using hPSC-derived in vitro 3D models of brain development and neurodegenerative disorders and discuss the advantages and challenges of employing 3D human brain models for drug screening and investigating mechanisms behind these neurological disorders.
2D hPSC neural differentiation models
2D neural tube-like rosettes, made up of neural progenitors organized radially around ventricle-like cavities, have previously been generated from hPSCs to study neuronal development [16–22]. Through specialized differentiation protocols, these 2D culture models have the ability to produce specified and highly homogeneous cell populations, such as excitatory and inhibitory cortical neurons [16, 17, 20], midbrain dopaminergic neurons [18, 19], and motor neurons [19, 21, 22]. Recently, Kindberg et al. were able to mimic neocortical development in 2D culture conditions to generate both glutamatergic and GABAergic cortical neurons on radial glial scaffolding by employing neocortical trophic factors FGF18, BDNF, and NT3 . Notably, the glutamatergic projection neurons were generated in a temporally sensitive manner mimicking in vivo neocortical development, with deep-layer CTIP2+ neurons appearing first, followed by generation of upper-layer SATB2+ neurons. Moreover, Lee et al. have developed a 2D neural culture system for producing connections between neurons from two distinct brain regions, the neocortex and midbrain . However, the inability of these 2D models to recapitulate in vivo-like cytoarchitectural organization and synaptic connections of the brain has impeded their use in precise disease modeling or drug screening applications.
hPSC-derived 3D neural cell aggregates
A variety of protocols are available for differentiating hPSCs into 3D neural cell aggregates. The 3D human neural model developed by Hogberg et al.  employs similar approaches to those established for 2D rosette culture . 3D neural cell aggregates can be generated from either single human neural progenitors or neural rosettes cultured in a 3D suspension, although more efficient neural induction has been observed in the single cell-derived aggregated cultures than in rosette-derived cultures. Kim et al. generated 3D neural cell aggregates instead by embedding immortalized human neural precursors in Matrigel containing high levels of neural-based extracellular matrix proteins [26, 27]. Furthermore, using genetically modified human neural precursors overexpressing mutant APP and PSEN1, their 3D model successfully mimicked β-amyloid (Aβ) accumulation, previously suggested to be associated with AD pathogenesis [28, 29]. Both Aβ deposition and Aβ-driven neurofibrillary tangles formation were observed in 3D aggregates but not in 2D cultures, possibly due to the more limited diffusion of Aβ in tightly-packed 3D aggregates than in 2D cultures [26, 27]. Although 3D neural aggregates provide the potential for neurodegenerative disease modeling, the lack of cytoarchitectural organization or cellular diversity in these 3D models will impede the recapitulation of complex brain development processes that are dependent on cell-cell interactions across different brain regions.
hPSC-derived 3D brain organoids
Human PSC-derived 3D brain organoid models
Brain region in organoid
Type of PSCs
# of VZ-like regions in organoid
Days of differentiation
Rostral and caudal cortices, hem and choroid plexus
Initial stage: Dkk-1, Lefty-1
Rostral cortices: Fgf8
Caudal cortices: Fgf inhibitor FGFR3-Fc
Cortical hem and choroid: Wnt3a and BMP4
Dorsal cortex, ventral forebrain, retina, hippocampus, choroid plexus, midbrain-hindbrain boundary
Forebrain, midbrain, hypothalamus
Forebrain organoids: dorsomorphine, A83–01, WNT3A, CHIR99021, SB-431542
Midbrain organoids: LDN-193189, SB-431542, SHH, purmorphamine, FGF-8, CHIR99021
Hypothalamus organoids: LDN-193189, SB-431542, 1-Thioglycerol, WNT3A, SHH, purmorphamine
Dorsomorphin, SB431542, bFGF, EGF
SB431542, LDN193189, PD0325901, bFGF, FGF18
Lancaster et al. used a modified SFEBq method to yield a novel hPSC-based 3D brain model called cerebral organoids . Also known as “mini-brains”, these cerebral organoids contain discrete brain regions including dorsal cortex, ventral forebrain, retina, hippocampus, choroid plexus, and midbrain-hindbrain boundary. To accomplish this, Lancaster et al. generated EBs from dissociated single hPSCs and once EBs had been neutrally induced to a neuroectodermal fate, they were embedded in Matrigel droplets and subsequently transferred to a spinning bioreactor for enhanced nutritional absorption, enabling the organoids to grow up to a few millimeters in diameter. Unlike the Eiraku et al. method , the Lancaster approach does not employ region-specific patterning factors to guide progenitors to particular regional identities. Instead, the use of an extracellular scaffolding matrix enables the growth and extension of neuroepithelium buds that will eventually self-organize and develop into various brain regions. Importantly, the use of Matrigel also introduces undefined animal factors into the culture that often leads to variations in organoid size and morphology and to inconsistent generation of brain regions in each organoid, thus limiting their potential to accurately model many aspects of neurodevelopment and degenerative diseases. Another source of inconsistency comes from the hPSC lines themselves, due to donor or manufacturing-induced variability that could impact reproducibility of cerebral organoids. Nevertheless, a subsequent study compared cell fate and regional specificities in cerebral organoids using single-cell RNA sequencing and showed that the organoid cortical cells followed a gene expression program that closely mimicked that of the human fetal neocortex . Thus, the 3D cerebral organoid model may be useful to explore the genetic underpinnings of human corticogenesis.
To reduce cost and space requirements of the Lancaster et al. spinning reactor approach, Qian et al. developed a miniature spinning bioreactor, named SpinΩ, that fits in a 12-well tissue culture plate and allows for larger scale generation of cerebral organoids . Furthermore, unlike the Eiraku and Lancaster approaches [32, 34], Qian et al. pre-patterned EBs formed from intact hPSC colonies using brain region-specific patterning factors for 7 days. The EBs were then embedded in Matrigel for another 7 days, removed from the Matrigel, and further cultured in SpinΩ. This approach enhanced organoid culture reproducibility and successfully generated different brain region-specific organoids, including forebrain, midbrain and hypothalamic organoids.
Paşca et al., on the other hand, successfully demonstrated the shift from neurogenesis to gliogenesis in hPSC-based 3D culture using a novel organoid model named human cortical spheroids (hCSs) . The hCS protocol begins with differentiating intact suspended hPSC colonies into the folded spherical structures using BMP and TGF-β signaling inhibitors, dorsomorphin and SB-431542, respectively. The floating spheroids are exposed to bFGF and epidermal growth factor (EGF) for 19 days to prime neural progenitors for the generation of nonreactive astrocytes, which appear after the peak of neurogenesis [38, 39]. Neurons in hCSs are surrounded by nonreactive astrocytes which facilitate formation of functional synapses . Furthermore, hCSs are made up of neurons from deep and superficial cortical layers and their transcriptional signatures mimic those found during in vivo fetal development. Therefore, hCSs provide a new avenue to model the patterning and specification of various neuronal and glial cell types.
Modeling developmental disorders with 3D brain organoids
3D iPSC-derived brain organoids from patients with neurodevelopmental disorders can recapitulate pathological phenotype in a dish. In addition, hPSC-derived 3D brain organoids enabled in vitro studies of cytoarchitectural changes in the embryonic neocortex as a result of perinatal teratogen exposure such as viruses and drugs. In this section, we summarize recent findings using hPSC-based 3D brain organoids for neurodevelopmental disease modeling and therapy development strategies.
Autosomal recessive primary microcephaly (MCPH) is a neurodevelopmental disorder characterized by smaller brain, particularly affecting cerebral cortex size [41, 42]. Twelve genes have been implicated in MCPH and the majority of them encode centrosomal proteins that play a role in mitotic progression . Among these MCPH genes, CDK5RAP2 regulates centriole replication and loss of CDK5RAP2 has been shown to impact proliferation of neural progenitors [43, 44]. Nevertheless, CDK5RAP2 mutant mice did not exhibit a severely reduced brain size as was observed in human patients [43, 45]. Lancaster et al. used iPSCs derived from a microcephalic patient with a heterozygous nonsense mutation in CDK5RAP2 to model the progression of microcephaly in 3D cerebral organoids . Compared to control, patient-derived organoids displayed smaller neuroepithelial regions, altered spindle orientation of radial glial cells, and abundant neuronal outgrowth. Importantly, patient-derived cerebral organoids overall were smaller in size compared to controls, similarly to the reduced brain size seen in patients. Their findings further indicated that smaller organoid size is a consequence of impaired proliferation and expansion of the founder progenitor pool and simultaneous premature neuronal differentiation. Their data also suggest that mouse neural progenitors prior to neurogenesis do not proliferate and expand to the same degree as in humans which might explain why CDK5RAP2-deficient mice do not exhibit microcephaly with the same severity as humans [46, 47].
Zika virus and microcephaly
Recently, the Zika virus (ZIKV) outbreak and the causal relationship between ZIKV infection during pregnancy and microcephaly in newborns had spurred the World Health Organization (WHO) to declare a global public health emergency [48–51]. ZIKV has been identified in the placenta, amniotic fluid, blood, and brains of microcephalic fetuses [49, 50, 52–55]. Nevertheless, the impact and mechanisms underlying the adverse neurodevelopmental effects of ZIKV are still largely unknown. Recently, ZIKV was shown to infect hiPSC-derived neural progenitors in 2D culture, and infected human neural progenitors were found to release infectious ZIKV particles . Moreover, several recent studies using 3D hPSC-derived cerebral organoids showed that ZIKV infection causes an overall reduction in organoid size, including a significant decrease in both ventricular zone (VZ) and cortical plate thickness [36, 57–59]. They also demonstrated that ZIKV induced cell death in neuroepithelial progenitors, attenuated the proliferation of progenitor cells, and increased the size of lumen within ventricular structures. These results are consistent with a clinical case report describing enlarged ventricles observed in a ZIKV-infected human fetal brain . Dang et al. indicated that Toll-like-Receptor 3 (TLR3), the innate immune receptor, was upregulated in hPSC-derived cerebral organoids infected with ZIKV, and blockade of TLR3 reduced ZIKV-induced organoid shrinkage and apoptosis . Transcriptomic analysis further revealed potential genes regulated by TLR3, including NTN1 and EPHB2 by which ZIKV may cause apoptosis and impact neurogenesis in the developing cerebral organoids. Intriguingly, both ZIKV and dengue virus, members of the flavivirus genus, have been shown to lead to activation of innate immune responses and increase the expression of TLR3 [61, 62]; however, only ZIKV would cause microcephaly [63, 64] and induce the adverse effects observed in 3D organoid culture . Therefore, further studies will be required in order to clarify a causal relationship between TLR3-mediated innate immune response and ZIKV-induced microcephaly.
Perinatal drug exposure and brain development
Prenatal exposure to many illicit drugs, alcohol, and tobacco has the potential to impact fetal brain development and continues to be a significant public health problem [65–68]. Of these substances, cocaine exposure during fetal development is most consistently linked to impairments of fetal head growth and subsequent neurobehavioral defects [67, 69–73]. Notably, it has been suggested that prenatal cocaine exposure is associated with impaired neurobehavioral function through disruption of frontal cortical development [74–76]. Lee et al. demonstrated that prenatal cocaine exposure during the most active period of neural progenitor proliferation induced deleterious cytoarchitectural changes in the embryonic neocortex in rats . These cytoarchitectural changes are initiated by N-oxidative metabolism of cocaine and consequent oxidative ER stress signaling . However, because of human and rodent interspecies differences in neocorticogenesis [79, 80] and CYP-mediated drug metabolism , findings from rodent models have been difficult to extrapolate to human brain development. Using a 2D hPSC-based neocortical model, Kindberg et al. demonstrated that cocaine caused premature neuronal differentiation of various cortical neuronal subtypes and impaired neocortical patterning . Using hPSC-based 3D neocortical organoids with a single cortex-like unit, they further identified a specific human cytochrome P450 isoform, CYP3A5, to be responsible for cocaine-induced developmental abnormalities of the human neocortex, which include both proliferation deficit and premature differentiation of neuroepithelial progenitors along with a significant reduction in cortical plate formation in organoids . 3D organoid methodology may therefore provide an alternative approach to study adverse effects of abused psychostimulants.
Autism spectrum disorder
Autism spectrum disorder (ASD) is a complex disorder of brain development characterized by language deficits, social communication difficulties, and repetitive behaviors. MRI scans and post-mortem studies of ASD patients indicate abnormal brain development owing to initial brain overgrowth followed by premature growth arrest, and these alterations are most evident in the prefrontal cortex [82–85]. Alterations in cerebral development in ASD lead to disorganization of cortical laminar architecture and changes in cortical networks that may result in autistic behaviors.
The majority of cases of ASD are idiopathic, therefore the exact genetic causes of autism are not known. Recently, Mariani et al. directly modeled early ASD brain development using iPSC-derived cerebral organoids from idiopathic ASD patients with common macrocephalic phenotype . ASD-derived organoids exhibited a decrease in cell cycle length in neural progenitor cells during the early stages of organoid differentiation, enhanced synaptic maturation, and overproduction of GABAergic inhibitory neurons. Their findings further demonstrated that FOXG1 and its downstream genes were responsible for the phenotypic abnormalities identified in ASD-derived organoids. Using a 2D neural differentiation model, another study with macrocephaly ASD revealed enhanced proliferation of neural progenitor cells resulting from dysregulation of WNT transcriptional cascade, and defects in the neuronal networks that could be reversed by insulin growth factor 1 (IGF-1), a drug currently in clinical trials for ASD .
Although the exact genetic causes of ASD are unknown, it is thought that several complex genetic factors might be involved. Copy-number variations (CNVs) are the most common recognized genetic variations associated with autism, and approximately 10–20% of autism subjects exhibit at least one CNV [15, 88, 89]. Yet, the impact of each particular CNV on brain development or function in autism is not known. A number of studies have linked CNVs to autism, including several CNVs on chromosome 17 at or near 17q21.31–21.32. Notably, one autistic subject was identified with a de novo mutation comprising of a duplication of 17q21.31-17q21.32/WNT3-WNT9B . Lee et al. recently reported that hPSC lines with a duplication of 17q21.31-17q21.32, including WNT3 and WNT9B, exhibited enhanced proliferation of early hPSC-derived neural progenitor cells, and increased neuronal differentiation at later culture stages . Their findings support Marchetto et al.’s assumption that WNT signaling is implicated in the pathogenesis of autism . These findings demonstrate the potential of using hiPSC technology to study aberrant neurodevelopmental processes in complex human diseases exhibiting heterogeneity in genotypes, such as ASD. Advancements in 3D cerebral organoid systems may provide an innovative platform for finding effective treatments for autism, potentially targeting WNT pathways.
Modeling neurodegenerative disorders with 3D brain organoids
Most neurodegenerative disorders first appear in adulthood. To recapitulate late-onset diseases, the fetal nature of hPSC-derived neural cells must be taken into consideration. In some cases, earlier-onset neurologic disease variants such as early-onset AD are available for hiPSC-based disease modeling [91, 92]. Recently, using early-onset familial Alzheimer’s (fAD) patient-derived iPSCs with APP duplication, Raja et al. recapitulated Alzheimer’s disease phenotypes including β-amyloid (Aβ) aggregation, hyperphosphorylated Tau (pTau), and endosome abnormalities in fAD patient-derived 3D brain organoids . Moreover, both amyloid and pTau pathologies in fAD organoids were reduced by treatment with β- and γ-secretase inhibitors. Therefore, 3D brain organoids may be a useful tool for drug screening for neurodegenerative disorders.
Nevertheless, early-onset fAD is a rare form of AD, accounting for less than 5% of all cases [91, 92]. In order to model late-onset neurologic diseases, the immature features of hPSC-derived brain organoids must be overcome. Long-term differentiation is a rational approach for further development of mature brain organoids, yet lack of vascularization seems to limit their prolonged growth potential. Vascularization does not participate in early development of the neocortex but is involved in regulating neurogenesis and guiding neuronal migration into the neocortex during the late stages of cortical development [94–96]. Moreover, impaired passage of nutrient and oxygen deep within organoids leads to necrosis at the center and interferes with organoid maturation. Absence of immune cells is another limitation and restricts the use of brain organoids in modeling inflammatory responses to infection or toxic substances, and aging-associated inflammation. It is worth noting that, due to the lack of vasculature and immune cells, the ability of hPSC-based 3D brain organoid model to mimic both young and aged brains remains controversial.
Potential improvements of hPSC-based 3D brain organoid model
The structural complexity of the human brain makes it difficult to detect all pathological conditions of neurological disorders in vitro. Recruitment of vascular networks, immune cells, or even blood-brain barrier within organoids would be beneficial for their advanced growth and differentiation and make them a more physiologically relevant model of the human brain. Recently, Takebe et al. demonstrated that transplantation of organ buds generated using brain tissues with mesenchymal stem cells facilitated organoid vascularization . Moreover, microglia, the only resident immune cells of the CNS, are suggested to regulate synaptic pruning and thus contribute to the development and maintenance of neural circuits; they have also been linked to early progression of neurodegenerative disease [98–103]. Microglia are absent from hPSC-derived brain organoids due to an embryonic origin distinct from that of neural progenitors. Microglia are derived from primitive myeloid progenitors in the embryonic yolk sac, and invade the brain during embryonic development [104–106]. Muffat et al. and Pandya et al. established protocols for efficient generation of microglia from hPSCs, which suggests a possibility for microglia to be incorporated into brain organoid cultures during early stages of organoid development [107, 108]. Recently, Schwartz et al. combined hPSC-derived neural progenitors, endothelial cells, mesenchymal stem cells, and microglia precursors on chemically defined polyethylene glycol hydrogels to establish 3D neural constructs with microglia and vascular network . These findings emphasize the importance of developing vascularized brain organoids and support the feasibility of introducing microglia into developing brain organoids to enhance neuronal maturation within these organoids.
Therapeutic development strategies of neurological disorders using 3D brain organoid technology
Modeling neurological disorders with 3D brain organoids derived from human pluripotent stem cells
Type of PSCs
Disease phenotype in organoid
Smaller neuroepithelial regions, altered spindle orientation of radial glial cells, abundant neuronal outgrowth, smaller organoid size
Heterozygous nonsense mutation in CDK5RAP2
Reintroducing CDK5RAP2 protein
Impaired brain growth
Increased cell death and suppressed proliferation of neural progenitors, decreased neuronal layer thickness and organoid size, enlarged lumen/ventricles
Impaired brain growth
Attenuated brain organoid growth
TLR3-mediated dysregulation of neurogenesis
Impaired brain growth
Reduced viability and growth of neural progenitor cells, smaller brain organoid size
Impaired brain growth
Increased apoptosis in neural progenitors, reduction of prolifration zone, disrupted cortical layers
Impaired brain growth
Proliferation inhibition of neuroepithelial progenitors, premature neuronal differentiation, reduction in cortical plate formation
CYP3A5-mediated cocaine oxidative metabolism
CYP3A5 inhibitor/Knockdown of CYP3A5
Autism spectrum disorder/macrocephalic phenotype
Increased progenitor cell proliferation, enhanced synaptic maturation, overproduction of GABAergic inhibitory neurons
Overexpression of transcription factor FOXG1
Knockdown of FOXG1
Early-onset familial Alzheimer’s
β-amyloid (Aβ) aggregation, hyperphosphorylated Tau (pTau), endosome abnormalities
β- and γ-secretase inhibitors
In this review, we described past milestones and present state of the field of brain organoid research, paying particular attention to the ability of current models to recapitulate brain development and neurodegenerative disorders. Although our understanding of the self-organizing properties of brain organoids is rapidly advancing, the generation of individual, discrete brain regions remains a challenge. The absence of immune and vascular systems in cultured brain organoids not only impedes their growth and maturation but also limits their use in modeling certain neurological disorders. However, these deficiencies might be improved by alternative strategies applying newly developed systems, such as vascularized organ buds, microglia generated from hPSCs, or the incorporation of vascular and microglial components into neural constructs [97, 107–109]. In addition, microfluidic networks can be employed to deliver patterned signals to brain organoids to further enhance their development and maturation . Despite hurdles to be overcome, these near-physiological brain organoid models represent orthogonal approaches that have been able to recapitulate characteristics of various neurological disorders in a self-organized 3D human neural tissue. In combination with transcriptome profiling, brain organoids can be employed to study functional effects of the dynamic expression of genetic risk factors in neurodevelopmental and neurodegenerative diseases. Advancements in iPSC-derived brain organoids that authentically recapitulates human brain development could potentially make 3D brain organoid culture systems an innovative platform for developing effective treatments for neurological disorders, including the use of HTS assays to identify small molecules that target neurological diseases, transcriptome sequencing to identify diagnostic biomarkers and uncover the molecular mechanisms, and the use of genome editing of hiPSCs for personalized cell replacement therapy. Although much remains to be done, recent studies and results support the potential of iPSC-derived 3D brain organoids in modeling and facilitating treatment of neurological disorders.
The authors would like to thank Drs. Steven R. Bauer, Shyh-Ching Lo, Pei Zhang, Kyung Sung, Zhaohui Ye, and Carolyn Wilson (CBER, FDA) for critical reading and suggestions of the manuscript.
Availability of data and materials
Conception and design of text description: C-T Lee & R-F Shen. Drafting of the manuscript, tables, and figures: C-TL, RMB, WWW, & R-FS. All authors read and approved the final manuscript.
Ethics approval and consent to participate
The manuscript is a literature review concerning the status and potential medical applications of brain organoids derived from stem cells or iPSCs. There are no studies conducted which involved human subjects.
Consent for publication
The manuscript has been internally reviewed and cleared for publication by the Center for Biologics Evaluation and Research, Food and Drug Administration. The results quoted in the manuscript are all published data. Any opinion expressed is that of the authors, which does not represent or is endorsed by the FDA policies.
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Gonzalez-Mantilla AJ, Moreno-De-Luca A, Ledbetter DH, Martin CL. A cross-disorder method to identify novel candidate genes for developmental brain disorders. JAMA Psychiatry. 2016;73(3):275–83.PubMedPubMed CentralView ArticleGoogle Scholar
- Gratten J, Wray NR, Keller MC, Visscher PM. Large-scale genomics unveils the genetic architecture of psychiatric disorders. Nat Neurosci. 2014;17(6):782–90.PubMedPubMed CentralView ArticleGoogle Scholar
- Parikshak NN, Gandal MJ, Geschwind DH. Systems biology and gene networks in neurodevelopmental and neurodegenerative disorders. Nat Rev Genet. 2015;16(8):441–58.PubMedPubMed CentralView ArticleGoogle Scholar
- Bertram L, Tanzi RE. The genetic epidemiology of neurodegenerative disease. J Clin Invest. 2005;115(6):1449–57.PubMedPubMed CentralView ArticleGoogle Scholar
- Lee CT, Bendriem RM, Kindberg AA, Worden LT, Williams MP, Drgon T, et al. Functional consequences of 17q21.31/WNT3-WNT9B amplification in hPSCs with respect to neural differentiation. Cell Rep. 2015;10(4):616–32.PubMedPubMed CentralView ArticleGoogle Scholar
- Gandal MJ, Leppa V, Won H, Parikshak NN, Geschwind DH. The road to precision psychiatry: translating genetics into disease mechanisms. Nat Neurosci. 2016;19(11):1397–407.PubMedView ArticleGoogle Scholar
- McCarroll SA, Hyman SE. Progress in the genetics of polygenic brain disorders: significant new challenges for neurobiology. Neuron. 2013;80(3):578–87.PubMedPubMed CentralView ArticleGoogle Scholar
- Escott-Price V, Sims R, Bannister C, Harold D, Vronskaya M, Majounie E, et al. Common polygenic variation enhances risk prediction for Alzheimer’s disease. Brain. 2015;138(12):3673–84.PubMedPubMed CentralView ArticleGoogle Scholar
- la Cognata V, Morello G, D’Agata V, Cavallaro S. Copy number variability in Parkinson’s disease: assembling the puzzle through a systems biology approach. Hum Genet. 2017;136(1):13–37.PubMedView ArticleGoogle Scholar
- Wen Z, Nguyen HN, Guo Z, Lalli MA, Wang X, Su Y, et al. Synaptic dysregulation in a human iPS cell model of mental disorders. Nature. 2014;515:414–8.PubMedPubMed CentralView ArticleGoogle Scholar
- Brennand KJ, Simone A, Jou J, Gelboin-Burkhart C, Tran N, Sangar S, et al. Modelling schizophrenia using human induced pluripotent stem cells. Nature. 2011;473(7346):221–5.PubMedPubMed CentralView ArticleGoogle Scholar
- Chen H, Qian K, Du Z, Cao J, Petersen A, Liu H, et al. Modeling ALS with iPSCs reveals that mutant SOD1 misregulates neurofilament balance in motor neurons. Cell Stem Cell. 2014;14(6):796–809.PubMedPubMed CentralView ArticleGoogle Scholar
- Hibaoui Y, Grad I, Letourneau A, Sailani MR, Dahoun S, Santoni FA, et al. Modelling and rescuing neurodevelopmental defect of down syndrome using induced pluripotent stem cells from monozygotic twins discordant for trisomy 21. EMBO Mol Med. 2014;6(2):259–77.PubMedGoogle Scholar
- Ryan SD, Dolatabadi N, Chan SF, Zhang X, Akhtar MW, Parker J, et al. Isogenic human iPSC parkinson’s model shows nitrosative stress-induced dysfunction in MEF2-PGC1α transcription. Cell. 2013;155(6):1351–64.PubMedPubMed CentralView ArticleGoogle Scholar
- Lee CT, Freed WJ, Mash DC. CNVs in neurodevelopmental disorders. Oncotarget. 2015;6(21):18238–9.PubMedPubMed CentralView ArticleGoogle Scholar
- Zhang SC, Wernig M, Duncan ID, Brüstle O, Thomson JA. In vitro differentiation of transplantable neural precursors from human embryonic stem cells. Nat Biotechnol. 2001;19(12):1129–33.PubMedView ArticleGoogle Scholar
- Elkabetz Y, Panagiotakos G, Al Shamy G, Socci ND, Tabar V, Studer L. Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage. Genes Dev. 2008;22(2):152–65.PubMedPubMed CentralView ArticleGoogle Scholar
- Yan Y, Yang D, Zarnowska ED, Du Z, Werbel B, Valliere C, et al. Directed differentiation of dopaminergic neuronal subtypes from human embryonic stem cells. Stem Cells. 2005;23(6):781–90.PubMedPubMed CentralView ArticleGoogle Scholar
- Chambers SM, Fasano CA, Papapetrou EP, Tomishima M, Sadelain M, Studer L. Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat Biotechnol. 2009;27(3):275–80.PubMedPubMed CentralView ArticleGoogle Scholar
- Shi Y, Kirwan P, Smith J, Robinson HPC, Livesey FJ. Human cerebral cortex development from pluripotent stem cells to functional excitatory synapses. Nat Neurosci. 2012;15(3):477–86.PubMedView ArticleGoogle Scholar
- Li XJ, Du ZW, Zarnowska ED, Pankratz M, Hansen LO, Pearce RA, et al. Specification of motoneurons from human embryonic stem cells. Nat Biotechnol. 2005;23(2):215–21.PubMedView ArticleGoogle Scholar
- Lee H, Shamy GA, Elkabetz Y, Schofield CM, Harrsion NL, Panagiotakos G, et al. Directed differentiation and transplantation of human embryonic stem cell-derived motoneurons. Stem Cells. 2007;25(8):1931–9.PubMedView ArticleGoogle Scholar
- Kindberg AA, Bendriem RM, Spivak CE, Chen J, Handreck A, Lupica CR, et al. An in vitro model of human neocortical development using pluripotent stem cells: cocaine-induced cytoarchitectural alterations. Dis Model Mech. 2014;7(12):1397–405.PubMedPubMed CentralView ArticleGoogle Scholar
- Lee CT, Bendriem RM, Freed WJ. A new technique for modeling neuronal connectivity using human pluripotent stem cells. Restor Neurol Neurosci. 2015;33(3):347–56.PubMedPubMed CentralGoogle Scholar
- Hogberg HT, Bressler J, Christian KM, Harris G, Makri G, O'Driscoll C, et al. Toward a 3D model of human brain development for studying gene/environment interactions. Stem Cell Res Ther. 2013;4 Suppl 1:S4.PubMedView ArticleGoogle Scholar
- Choi SH, Kim YH, Hebisch M, Sliwinski C, Lee S, D'Avanzo C, et al. A three-dimensional human neural cell culture model of Alzheimer’s disease. Nature. 2014;515(7526):274–8.PubMedPubMed CentralView ArticleGoogle Scholar
- Kim YH, Choi SH, D'Avanzo C, Hebisch M, Sliwinski C, Bylykbashi E, et al. A 3D human neural cell culture system for modeling Alzheimer’s disease. Nat Protoc. 2015;10(7):985–1006.PubMedPubMed CentralView ArticleGoogle Scholar
- O’Brien RJ, Wong PC. Amyloid precursor protein processing and Alzheimer’s disease. Annu Rev Neurosci. 2011;34:185–204.PubMedPubMed CentralView ArticleGoogle Scholar
- Zhang H, Ma Q, Zhang Y, Xu H. Proteolytic processing of Alzheimer’s β-amyloid precursor protein. J Neurochem. 2012;120(Suppl 1):9–21.PubMedView ArticleGoogle Scholar
- Watanabe K, Ueno M, Kamiya D, Nishiyama A, Matsumura M, Wataya T, et al. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol. 2007;25(6):681–6.PubMedView ArticleGoogle Scholar
- Watanabe K, Kamiya D, Nishiyama A, Katayama T, Nozaki S, Kawasaki H, et al. Directed differentiation of telencephalic precursors from embryonic stem cells. Nat Neurosci. 2005;8(3):288–96.PubMedView ArticleGoogle Scholar
- Eiraku M, Watanabe K, Matsuo-Takasaki M, Kawada M, Yonemura S, Matsumura M, et al. Self-organized formation of polarized cortical tissues from ESCs and its active manipulation by extrinsic signals. Cell Stem Cell. 2008;3(5):519–32.PubMedView ArticleGoogle Scholar
- Kadoshima T, Sakaguchi H, Nakano T, Soen M, Ando S, Eiraku M, et al. Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex. Proc Natl Acad Sci U S A. 2013;110(50):20284–9.PubMedPubMed CentralView ArticleGoogle Scholar
- Lancaster MA, Renner M, Martin CA, Wenzel D, Bicknell LS, Hurles ME, et al. Cerebral organoids model human brain development and microcephaly. Nature. 2013;501(7467):373–9.PubMedView ArticleGoogle Scholar
- Camp JG, Badsha F, Florio M, Kanton S, Gerber T, Wilsch-Bräuninger M, et al. Human cerebral organoids recapitulate gene expression programs of fetal neocortex development. Proc Natl Acad Sci U S A. 2015;112(51):15672–7.PubMedPubMed CentralGoogle Scholar
- Qian X, Nguyen HN, Song MM, Hadiono C, Ogden SC, Hammack C, et al. Brain-region-specific Organoids using mini-bioreactors for modeling ZIKV exposure. Cell. 2016;165(5):1238–54.PubMedPubMed CentralView ArticleGoogle Scholar
- Paşca AM, Sloan SA, Clarke LE, Tian Y, Makinson CD, Huber N, et al. Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture. Nat Methods. 2015;12(7):671–8.PubMedPubMed CentralView ArticleGoogle Scholar
- Franco SJ, Müller U. Shaping our minds: stem and progenitor cell diversity in the mammalian Neocortex. Neuron. 2013;77(1):19–34.PubMedPubMed CentralView ArticleGoogle Scholar
- Shen Q, Wang Y, Dimos JT, Fasano CA. The timing of cortical neurogenesis is encoded within lineages of individual progenitor cells. Nature. 2006;9(6):743–51.Google Scholar
- Lee CT, Chen J, Kindberg AA, Bendriem RM, Spivak CE, Williams MP, et al. CYP3A5 mediates effects of cocaine on human neocorticogenesis: studies using an in vitro 3D self-organized hPSC model with a single cortex-like unit. Neuropsychopharmacology. 2017;42(3):774–84.PubMedView ArticleGoogle Scholar
- Faheem M, Naseer MI, Rasool M, Chaudhary AG, Kumosani TA, Ilyas AM, et al. Molecular genetics of human primary microcephaly: an overview. BMC Med Genet. 2015;8 Suppl 1:S4.Google Scholar
- Woods CG, Bond J, Enard W. Autosomal recessive primary microcephaly (MCPH): a review of clinical, molecular, and evolutionary findings. Am J Hum Genet. 2005;76(5):717–28.PubMedPubMed CentralView ArticleGoogle Scholar
- Barrera JA, Kao LR, Hammer RE, Seemann J, Fuchs JL, Megraw TL. CDK5RAP2 regulates centriole engagement and cohesion in mice. Dev Cell. 2010;18(6):913–26.PubMedPubMed CentralView ArticleGoogle Scholar
- Megraw TL, Sharkey JT, Nowakowski RS. Cdk5rap2 exposes the centrosomal root of microcephaly syndromes. Trends Cell Biol. 2011;21(8):470–80.PubMedPubMed CentralView ArticleGoogle Scholar
- Lizarraga SB, Margossian SP, Harris MH, Campagna DR, Han AP, Blevins S, et al. Cdk5rap2 regulates centrosome function and chromosome segregation in neuronal progenitors. Development. 2010;137(11):1907–17.PubMedPubMed CentralView ArticleGoogle Scholar
- Florio M, Huttner WB. Neural progenitors, neurogenesis and the evolution of the neocortex. Development. 2014;141(11):2182–94.PubMedView ArticleGoogle Scholar
- Lui JH, Hansen DV, Kriegstein AR. Development and evolution of the human neocortex. Cell. 2011;146(1):18–36.PubMedPubMed CentralView ArticleGoogle Scholar
- Martines RB, Bhatnagar J, de Oliveira Ramos AM, Davi HP, Iglezias SD, Kanamura CT, et al. Pathology of congenital Zika syndrome in Brazil: a case series. Lancet. 2016;388(10047):898–904.PubMedView ArticleGoogle Scholar
- Calvet G, Aguiar RS, Melo AS, Sampaio SA, de Filippis I, Fabri A, et al. Detection and sequencing of Zika virus from amniotic fluid of fetuses with microcephaly in Brazil: a case study. Lancet Infect Dis. 2016;16(6):653–60.PubMedView ArticleGoogle Scholar
- Mlakar J, Korva M, Tul N, Popović M, Poljšak-Prijatelj M, Mraz J, et al. Zika virus associated with microcephaly. N Engl J Med. 2016;374(10):951–8.PubMedView ArticleGoogle Scholar
- Heymann DL, Hodgson A, Sall AA, Freedman DO, Staples JE, Althabe F, et al. Zika virus and microcephaly: why is this situation a PHEIC? Lancet. 2016;387(10020):719–21.PubMedView ArticleGoogle Scholar
- Sarno M, Sacramento GA, Khouri R, do Rosário MS, Costa F, Archanjo G, et al. Zika virus infection and stillbirths: a case of hydrops fetalis, hydranencephaly and fetal demise. PLoS Negl Trop Dis. 2016;10(2):e0004517.PubMedPubMed CentralView ArticleGoogle Scholar
- Martines RB, Bhatnagar J, Keating MK, Silva-Flannery L, Muehlenbachs A, Gary J, et al. Notes from the field: evidence of zika virus infection in brain and placental tissues from two congenitally infected newborns and two fetal losses--Brazil, 2015. MMWR Morb Mortal Wkly Rep. 2016;65(6):159–60.PubMedView ArticleGoogle Scholar
- Ventura CV, Maia M, Bravo-Filho V, Góis AL, Belfort R. Zika virus in Brazil and macular atrophy in a child with microcephaly. Lancet. 2016;387(10015):228.PubMedView ArticleGoogle Scholar
- Oliveira Melo AS, Malinger G, Ximenes R, Szejnfeld PO, Alves Sampaio S, Bispo De Filippis AM. Zika virus intrauterine infection causes fetal brain abnormality and microcephaly: tip of the iceberg? Ultrasound Obstet Gynecol. 2016;47(1):6–7.PubMedView ArticleGoogle Scholar
- Tang H, Hammack C, Ogden SC, Wen Z, Qian X, Li Y, et al. Zika virus infects human cortical neural progenitors and attenuates their growth. Cell Stem Cell. 2016;18(5):587–90.PubMedPubMed CentralView ArticleGoogle Scholar
- Dang J, Tiwari SK, Lichinchi G, Qin Y, Patil VS, Eroshkin AM, et al. Zika virus depletes neural progenitors in human cerebral Organoids through activation of the innate immune receptor TLR3. Cell Stem Cell. 2016;19(2):258–65.PubMedPubMed CentralView ArticleGoogle Scholar
- Garcez PP, Loiola EC, Madeiro da Costa R, Higa LM, Trindade P, Delvecchio R, et al. Zika virus impairs growth in human neurospheres and brain organoids. Science. 2016;352(6287):816–8.PubMedView ArticleGoogle Scholar
- Cugola FR, Fernandes IR, Russo FB, Freitas BC, Dias JL, Guimarães KP, et al. The Brazilian Zika virus strain causes birth defects in experimental models. Nature. 2016;534(7606):267–71.PubMedPubMed CentralGoogle Scholar
- Driggers RW, Ho CY, Korhonen EM, Kuivanen S, Jääskeläinen AJ, Smura T, et al. Zika virus infection with prolonged maternal Viremia and fetal brain abnormalities. N Engl J Med. 2016;374(22):2142–51.PubMedView ArticleGoogle Scholar
- Surasombatpattana P, Hamel R, Patramool S, Luplertlop N, Thomas F, Desprès P, et al. Dengue virus replication in infected human keratinocytes leads to activation of antiviral innate immune responses. Infect Genet Evol. 2011;11(7):1664–73.PubMedView ArticleGoogle Scholar
- Hamel R, Dejarnac O, Wichit S, Ekchariyawat P, Neyret A, Luplertlop N, et al. Biology of Zika virus infection in human skin cells. J Virol. 2015;89(17):8880–96.PubMedPubMed CentralView ArticleGoogle Scholar
- Krauer F, Riesen M, Reveiz L, Oladapo OT, Martínez-Vega R, Porgo TV, et al. Zika virus infection as a cause of congenital brain abnormalities and Guillain-Barre syndrome: systematic review. PLoS Med. 2016;14(1):e1002203.View ArticleGoogle Scholar
- Pouliot SH, Xiong X, Harville E, Paz-Soldan V, Tomashek KM, Breart G, et al. Maternal dengue and pregnancy outcomes: a systematic review. Obstet Gynecol Surv. 2010;65(2):107–18.PubMedGoogle Scholar
- Minnes S, Lang A, Singer L. Prenatal tobacco, marijuana, stimulant, and opiate exposure: outcomes and practice implications. Addict Sci Clin Pract. 2011;6(1):57–70.PubMedPubMed CentralGoogle Scholar
- Thompson BL, Levitt P, Stanwood GD. Prenatal exposure to drugs: effects on brain development and implications for policy and education. Nat Rev Neurosci. 2009;10(4):303–12.PubMedPubMed CentralView ArticleGoogle Scholar
- Rivkin MJ, Davis PE, Lemaster JL, Cabral HJ, Warfield SK, Mulkern RV, et al. Volumetric MRI study of brain in children with intrauterine exposure to cocaine, alcohol, tobacco, and marijuana. Pediatrics. 2008;121(4):741–50.PubMedPubMed CentralView ArticleGoogle Scholar
- Behnke M, Smith VC. Committee on substance abuse, committee on fetus and newborn. Prenatal substance abuse: short- and long-term effects on the exposed fetus. Pediatrics. 2013;131(3):e1009–24.PubMedView ArticleGoogle Scholar
- Chiriboga CA, Brust JC, Bateman D, Hauser WA. Dose-response effect of fetal cocaine exposure on newborn neurologic function. Pediatrics. 1999;103(1):79–85.PubMedView ArticleGoogle Scholar
- Chiriboga CA, Kuhn L, Wasserman GA. Prenatal cocaine exposures and dose-related cocaine effects on infant tone and behavior. Neurotoxicol Teratol. 2007;29(3):323–30.PubMedView ArticleGoogle Scholar
- Delaney-Black V, Covington C, Ostrea E Jr, Romero A, Baker D, Tagle MT, et al. Prenatal cocaine and neonatal outcome: evaluation of dose-response relationship. Pediatrics. 1996;98(4 Pt 1):735–40.PubMedGoogle Scholar
- Frank DA, Augustyn M, Zuckerman BS. Neonatal neurobehavioral and neuroanatomic correlates of prenatal cocaine exposure. Problems of dose and confounding. Ann N Y Acad Sci. 1998;846:40–50.PubMedPubMed CentralView ArticleGoogle Scholar
- Tronick EZ, Frank DA, Cabral H, Mirochnick M, Zuckerman B. Late dose-response effects of prenatal cocaine exposure on newborn neurobehavioral performance. Pediatrics. 1996;98(1):76–83.PubMedPubMed CentralGoogle Scholar
- Rando K, Chaplin TM, Potenza MN, Mayes L, Sinha R. Prenatal cocaine exposure and gray matter volume in adolescent boys and girls: relationship to substance use initiation. Biol Psychiatry. 2013;74(7):482–9.PubMedPubMed CentralView ArticleGoogle Scholar
- Singer LT, Arendt R, Minnes S, Farkas K, Salvator A, Kirchner HL, et al. Cognitive and motor outcomes of cocaine-exposed infants. J Am Med Assoc. 2002;287(15):1952–60.View ArticleGoogle Scholar
- Bandstra ES, Vogel AL, Morrow CE, Xue L, Anthony JC. Severity of prenatal cocaine exposure and child language functioning through age seven years: a longitudinal latent growth curve analysis. Subst Use Misuse. 2004;39(1):25–59.PubMedPubMed CentralView ArticleGoogle Scholar
- Lee CT, Chen J, Worden LT, Freed WJ. Cocaine causes deficits in radial migration and alters the distribution of glutamate and GABA neurons in the developing rat cerebral cortex. Synapse. 2011;65(1):21–34.PubMedPubMed CentralView ArticleGoogle Scholar
- Lee CT, Chen J, Hayashi T, Tsai SY, Sanchez JF, Errico SL, et al. A mechanism for the inhibition of neural progenitor cell proliferation by cocaine. PLoS Med. 2008;5(6):e117.PubMedPubMed CentralView ArticleGoogle Scholar
- Rakic P. Evolution of the neocortex: a perspective from developmental biology. Nat Rev Neurosci. 2009;10(10):724–35.PubMedPubMed CentralView ArticleGoogle Scholar
- Hill RS, Walsh CA. Molecular insights into human brain evolution. Nature. 2005;437(7055):64–7.PubMedView ArticleGoogle Scholar
- Martignoni M, Groothuis GM, de Kanter R. Species differences between mouse, rat, dog, monkey and human CYP-mediated drug metabolism, inhibition and induction. Expert Opin Drug Metab Toxicol. 2006;2(6):875–94.PubMedView ArticleGoogle Scholar
- Courchesne E, Mouton PR, Calhoun ME, Semendeferi K, Ahrens-Barbeau C, Hallet MJ, et al. Neuron number and size in prefrontal cortex of children with autism. JAMA. 2011;306(18):2001–10.PubMedView ArticleGoogle Scholar
- Stoner R, Chow ML, Boyle MP, Sunkin SM, Mouton PR, Roy S, et al. Patches of disorganization in the neocortex of children with autism. N Engl J Med. 2014;370(13):1209–19.PubMedPubMed CentralView ArticleGoogle Scholar
- Amaral DG, Schumann CM, Nordahl CW. Neuroanatomy of autism. Trends Neurosci. 2008;31(3):137–45.PubMedView ArticleGoogle Scholar
- Zielinski BA, Prigge MB, Nielsen JA, Froehlich AL, Abildskov TJ, Anderson JS, et al. Longitudinal changes in cortical thickness in autism and typical development. Brain. 2014;137(Pt 6):1799–812.PubMedPubMed CentralView ArticleGoogle Scholar
- Mariani J, Coppola G, Zhang P, Abyzov A, Provini L, Tomasini L, et al. FOXG1-dependent dysregulation of GABA/glutamate neuron differentiation in autism spectrum disorders. Cell. 2015;162(2):375–90.PubMedPubMed CentralView ArticleGoogle Scholar
- Marchetto MC, Belinson H, Tian Y, Freitas BC, Fu C, Vadodaria KC, et al. Altered proliferation and networks in neural cells derived from idiopathic autistic individuals. Mol Psychiatry. 2016; doi:10.1038/mp.2016.95.
- Abrahams BS, Geschwind DH. Advances in autism genetics: on the threshold of a new neurobiology. Nat Rev Genet. 2008;9(5):341–55.PubMedPubMed CentralView ArticleGoogle Scholar
- Merikangas AK, Corvin AP, Gallagher L. Copy-number variants in neurodevelopmental disorders: promises and challenges. Trends Genet. 2009;25(12):536–44.PubMedView ArticleGoogle Scholar
- Girirajan S, Brkanac Z, Coe BP, Baker C, Vives L, Vu TH, et al. Relative burden of large CNVs on a range of neurodevelopmental phenotypes. PLoS Genet. 2011;7(11):e1002334.PubMedPubMed CentralView ArticleGoogle Scholar
- Campion D, Dumanchin C, Hannequin D, Dubois B, Belliard S, Puel M, et al. Early-onset autosomal dominant Alzheimer disease: prevalence, genetic heterogeneity, and mutation spectrum. Am J Hum Genet. 1999;65(3):664–70.PubMedPubMed CentralView ArticleGoogle Scholar
- Blennow K, de Leon MJ, Zetterberg H. Alzheimer’s disease. Lancet. 2006;368(9533):387–403.PubMedView ArticleGoogle Scholar
- Raja WK, Mungenast AE, Lin YT, Ko T, Abdurrob F, Seo J, et al. Self-organizing 3D human neural tissue derived from induced pluripotent stem cells recapitulate Alzheimer’s disease phenotypes. PLoS One. 2016;11(9):e0161969.PubMedPubMed CentralView ArticleGoogle Scholar
- Vasudevan A, Long JE, Crandall JE, Rubenstein JLR, Bhide PG. Compartment-specific transcription factors orchestrate angiogenesis gradients in the embryonic brain. Nat Neurosci. 2008;11(4):429–39.PubMedPubMed CentralView ArticleGoogle Scholar
- Javaherian A, Kriegstein A. A stem cell niche for intermediate progenitor cells of the embryonic cortex. Cereb Cortex. 2009;19(Suppl 1):i70–7.PubMedPubMed CentralView ArticleGoogle Scholar
- Lange C, Turrero Garcia M, Decimo I, Bifari F, Eelen G, Quaegebeur A, et al. Relief of hypoxia by angiogenesis promotes neural stem cell differentiation by targeting glycolysis. EMBO J. 2016;35(9):924–41.PubMedPubMed CentralView ArticleGoogle Scholar
- Takebe T, Enomura M, Yoshizawa E, Kimura M, Koike H, Ueno Y, et al. Vascularized and complex organ buds from diverse tissues via mesenchymal cell-driven condensation. Cell Stem Cell. 2015;16(5):556–65.PubMedView ArticleGoogle Scholar
- Wake H, Moorhouse AJ, Miyamoto A, Nabekura J. Microglia: actively surveying and shaping neuronal circuit structure and function. Trends Neurosci. 2013;36(4):209–17.PubMedView ArticleGoogle Scholar
- Salter MW, Beggs S. Sublime microglia: expanding roles for the guardians of the CNS. Cell. 2014;158(1):15–24.PubMedView ArticleGoogle Scholar
- Cunningham CL, Martinez-Cerdeno V, Noctor SC. Microglia regulate the number of neural precursor cells in the developing cerebral cortex. J Neurosci. 2013;33(10):4216–33.PubMedPubMed CentralView ArticleGoogle Scholar
- Schafer DP, Lehrman EK, Kautzman AG, Koyama R, Mardinly AR, Yamasaki R, et al. Microglia sculpt postnatal neural circuits in an activity and complement-dependent manner. Neuron. 2012;74(4):691–705.PubMedPubMed CentralView ArticleGoogle Scholar
- Hong S, Beja-Glasser VF, Nfonoyim BM, Frouin A, Li S, Ramakrishnan S, et al. Complement and microglia mediate early synapse loss in Alzheimer mouse models. Science. 2016;352(6286):712–6.PubMedPubMed CentralView ArticleGoogle Scholar
- Hong S, Dissing-Olesen L, Stevens B. New insights on the role of microglia in synaptic pruning in health and disease. Curr Opin Neurobiol. 2016;36:128–34.PubMedView ArticleGoogle Scholar
- Tay TL, Savage JC, Hui CW, Bisht K, Tremblay ME. Microglia across the lifespan: from origin to function in brain development, plasticity and cognition. J Physiol. 2017;595(6):1929–45.PubMedView ArticleGoogle Scholar
- Ginhoux F, Greter M, Leboeuf M, Nandi S, See P, Gokhan S, et al. Fate mapping analysis reveals that adult microglia derive from primitive macrophages. Science. 2010;330(6005):841–5.PubMedPubMed CentralView ArticleGoogle Scholar
- Ginhoux F, Lim S, Hoeffel G, Low D, Huber T. Origin and differentiation of microglia. Front Cell Neurosci. 2013;7:45.PubMedPubMed CentralView ArticleGoogle Scholar
- Muffat J, Li Y, Yuan B, Mitalipova M, Omer A, Corcoran S, et al. Efficient derivation of microglia-like cells from human pluripotent stem cells. Nat Med. 2016;22(11):1358–67.PubMedPubMed CentralView ArticleGoogle Scholar
- Pandya H, Shen MJ, Ichikawa DM, Sedlock AB, Choi Y, Johnson KR, et al. Differentiation of human and murine induced pluripotent stem cells to microglia-like cells. Nat Neurosci. 2017; doi:10.1038/nn.4534.
- Schwartz MP, Hou Z, Propson NE, Zhang J, Engstrom CJ, Santos Costa V, et al. Human pluripotent stem cell-derived neural constructs for predicting neural toxicity. Proc Natl Acad Sci U S A. 2015;112(40):12516–21.PubMedPubMed CentralView ArticleGoogle Scholar
- van der Worp HB, Howells DW, Sena ES, Porritt MJ, Rewell S, O'Collins V, et al. Can animal models of disease reliably inform human studies? PLoS Med. 2010;7(3):e1000245.PubMedPubMed CentralView ArticleGoogle Scholar
- Inglese J, Johnson RL, Simeonov A, Xia M, Zheng W, Austin CP, et al. High-throughput screening assays for the identification of chemical probes. Nat Chem Biol. 2007;3(8):466–79.PubMedView ArticleGoogle Scholar
- Ebert AD, Svendsen CN. Human stem cells and drug screening: opportunities and challenges. Nat Rev Drug Discov. 2010;9(5):367–72.PubMedView ArticleGoogle Scholar
- Engle SJ, Vincent F. Small molecule screening in human induced pluripotent stem cell-derived terminal cell types. J Biol Chem. 2014;289(8):4562–70.PubMedView ArticleGoogle Scholar
- Byron SA, Van Keuren-Jensen KR, Engelthaler DM, Carpten JD, Craig DW. Translating RNA sequencing into clinical diagnostics: opportunities and challenges. Nat Rev Genet. 2016;17(5):257–71.PubMedView ArticleGoogle Scholar
- Rabbani B, Nakaoka H, Akhondzadeh S, Tekin M, Mahdieh N. Next generation sequencing: implications in personalized medicine and pharmacogenomics. Mol BioSyst. 2016;12(6):1818–30.PubMedView ArticleGoogle Scholar
- Pinto AM, Ariani F, Bianciardi L, Daga S, Renieri A. Exploiting the potential of next-generation sequencing in genomic medicine. Expert Rev Mol Diagn. 2016;16(9):1037–47.PubMedView ArticleGoogle Scholar
- Hendriks WT, Warren CR, Cowan CA. Genome editing in human pluripotent stem cells: approaches, pitfalls, and solutions. Cell Stem Cell. 2016;18(1):53–65.PubMedPubMed CentralView ArticleGoogle Scholar
- Smith C, Ye Z, Cheng L. Genome editing in human pluripotent stem cells. Cold Spring Harb Protoc. 2016;(4):pdb.top086819.Google Scholar
- Yin X, Mead BE, Safaee H, Langer R, Karp JM, Levy O. Engineering stem cell organoids. Cell Stem Cell. 2016;18(1):25–38.PubMedPubMed CentralView ArticleGoogle Scholar
- Orqueda AJ, Giménez CA, Pereyra-Bonnet F. iPSCs: a minireview from bench to bed, including organoids and the CRISPR system. Stem Cells Int. 2016;2016:5934782.PubMedPubMed CentralView ArticleGoogle Scholar
- Nie J, Hashino E. Organoid technologies meet genome engineering. EMBO Rep. 2017;18(3):367–76.PubMedView ArticleGoogle Scholar